作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1810-1817.doi: 10.3724/SP.J.1006.2012.01810
赵亮,蔡彩平,梅鸿献,郭旺珍*
ZHAO Liang,CAI Cai-Ping,MEI Hong-Xian,GUO Wang-Zhen*
摘要:
保守性强、重复性好、多态性高的微卫星位点可被有效用于构建作物DNA条形码。选取目前生产上主要推广种植、代表不同来源系统的12个棉花品种作为微卫星位点筛选材料,参考我室构建的四倍体栽培棉种种间高密度遗传图谱信息,从376对覆盖全基因组的SSR引物中,筛选出51对引物可扩增出带型清晰且多态性高的微卫星位点。这些引物在12个供试品种中共产生155个等位位点,每对引物揭示的等位基因位点在2~7之间,平均值为3.04。参照微卫星位点的染色体定位和多态信息,在每条染色体上选择一个多态性相对高的SSR位点,其相应的26对SSR引物被推荐为构建棉花品种DNA条形码的一套首选引物,并初步应用于12个品种的DNA条形码编制。其余25对引物作为候选引物。使用该套引物扩增出的微卫星位点可用于大量棉花品种DNA条形码构建,为棉花品种真实性和纯度的分子鉴定奠定基础。
[1]Liu X-L(刘新龙), Ma L(马丽), Chen X-K(陈学宽), Ying X-M(应雄美), Cai Q(蔡青), Liu J-Y(刘家勇), Wu C-W(吴才文). Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan, China. Acta Agron Sin (作物学报), 2010, 36(2): 202−210 (in Chinese with English abstract)[2]Kuang M(匡猛), Yang W-H(杨伟华), Xu H-X(许红霞), Wang Y-Q(王延琴), Zhou D-Y(周大云), Feng X-A(冯新爱) Construction of DNA fingerprinting and analysis of genetic diversity with SSR markers for cotton major cultivars in china. Sci Agric Sin (中国农业科学), 2011, 44(1): 20−27 (in Chinese with English abstract)[3]Kashi Y, King D, Soller M. Simple sequence repeats as a source of quantitative genetic variation. Trends Genet, 1997, 13: 74–78[4]Röder M S, Korzun V, Gill B S, Ganal M W. The physical mapping of microsatellite markers in wheat. Genome, 1998, 41: 278–283[5]Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007–2023[6]Yan J-W(颜静宛), Tian D-G(田大刚), Xu Y(许彦), Wang F(王锋). Constructing SSR molecular database for identity system of hybrid rice parents. Fujian J Agric Sci (福建农业学报), 2011, 26( 2): 148−152 (in Chinese with English abstract)[7]Zhao X-Y(赵新燕), Huang L(黄莉), Ren X-P(任小平), Jiang H-F(姜慧芳), Chen Y-N(陈玉宁). Establishment of DNA fingerprint identity of arachis species with high oil content. Acta Agric Boreali-Sin (华北农学报) 2010, 25(6): 64−70 (in Chinese with English abstract)[8]Ai C-X(艾呈祥), Zhang L-S(张力思), Wei H-R(魏海蓉), Yuan K-J(苑克俊), Jin S-N(金松南), Liu Q-Z(刘庆忠). Construction of molecular fingerprinting database for sweet cherry using SSR markers. Chin Agric Sci Bull (中国农学通报), 2007, 23(5): 55–58 (in Chinese with English abstract)[9]Guo W Z, Cai C P, Wang C B, Zhao L, Wang L, Zhang T Z. A preliminary analysis of genome structure and composition in Gossypium hirsutum. BMC Genom, 2008, 9: 313[10]Rong J, Abbey C, Bowers J E, Brubaker C L, Chang C, Chee P W, Delmonte T A, Ding X, Garza J J, Marler B S, Park C H, Pierce G J, Rainey K M, Rastogi V K, Schulze S R, Trolinder N L, Wendel J F, Wilkins T A, Williams-Coplin T D, Wing R A, Wright R J, Zhao X, Zhu L, Paterson A H. A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics, 2004, 166: 389–417[11]Nguyen T B, Giband M, Brottier P, Risterucci A M, Lacape J M. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet, 2004, 109: 167–175[12]Frelichowski J E Jr, Palmer M B, Main D, Tomkins J P, Cantrell R G, Stelly D M, Yu J, Kohel R J, Ulloa M. Cotton genome mapping with new microsatellites from Acala ‘Maxxa’ BAC-ends. Mol Gen Genom, 2006, 275: 479–491[13]Wu Y-T(武耀廷), Zhang T-Z(张天真), Yin J-M(殷剑美). Genetic diversity detected by DNA markers and phenotypes in upland cotton. J Gen Genom (遗传学报), 2001, 28(11): 1040–1050 (in Chinese with English abstract)[14]Zhu L-F(朱龙付), Zhang X-L(张献龙), Nie Y-C(聂以春). Analysis of genetic diversity in upland cotton (Gossypium hirsutum L.) Cultivars from China and foreign countries by RAPDs and SSRs. J Agric Biotechnol (农业生物技术学报), 2003, 11(5): 450–455 (in Chinese with English abstract)[15]Liu S, Cantrell R G, McCarty J C, Stewart J M. Simple sequence repeat-based assessment of genetic diversity in cotton race stock accessions. Crop Sci, 2000, 40: 1459–1469 [16]Zhao J-R(赵久然), Wang F-G(王凤格), Guo J-L(郭景伦), Chen G(陈刚), Liao Q(廖琴), Sun S-X(孙世贤), Chen R-M(陈如明), Liu L-Z(刘龙洲). Series of research on establishing DNA fingerprint pool of Chinese new maize cultivars: II. Confirmation of a set of SSR core primers pairs. J Maize Sci (玉米科学), 2003, 11(2): 3–5 (in Chinese with English abstract) [17]Huang Z-K(黄滋康). Cotton Vatieties and Their Genealogy in China (revised and enlarged edition)[中国棉花品种及其系谱(修订本)]. Beijing: China Agriculture press, 2007 (in Chinese)[18]Cotton Institute of Chinese Academy of Agriculture Sciences (中国农业科学院棉花研究所). Flora of Cotton Varieties in China (1978–2007)[中国棉花品种志(1978–2007)]. Beijing : China Agriculture Science Technology Press, 2009 (in Chinese)[19]Paterson A H, Brubaker C, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep, 1993, 11: 122–127[20]Zhang J, Wu Y T, Guo W Z, Zhang T Z. Fast screening of microsatellite markers in cotton with PAGE/silver staining. Cotton Sci, 2000, 12: 267–269 (in Chinese with English abstract) [21]Zhang J, Guo W Z, Zhang T Z. Molecular linkage map of allotetraploid cotton (Gossypium hirsutum L. × Gossypium barbadense L.) with a haploid population. Theor Appl Genet, 2002, 105: 1166–1174[22]Reddy O U K, Pepper A E, Abdurakhmonov I, Saha S, Jenkins J N, Brooks T, Bolek Y, El-Zik K M. New dinucleotide and trinucleotide microsatellite marker resources for cotton genome research. J Cotton Sci, 2001, 5: 103–113[23]Han Z G, Guo W Z, Song X L, Zhang T Z. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton. Mol Gen Genom, 2004, 272: 308–327[24]Park Y H, Alabady M S, Ulloa M, Sickler B, Wilkins T A, Yu J, Stelly D M, Kohel R J, El-Shihy O M, Cantrell R G. Genetic mapping of new cotton fiber loci using EST-derived microsatellites in an interspecific recombinant inbred (RIL) cotton population. Mol Gen Genom, 2005, 274: 428–441[25]Song X L, Wang K, Guo W Z, Zhang J, Zhang T Z. A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. × G. barbadense L. Genome, 2005, 48: 378–390[26]Han Z G, Wang C B, Song X L, Guo W Z, Gou J Y, Li C H, Chen X Y, Zhang T Z. Characteristics, development and mapping of Gossypium hirsutum derived EST-SSR in allotetraploid cotton. Theor Appl Genet, 2006, 112: 430–439[27]Yu X-Y(于新艳), Wang F-G(王凤格), Zhao J-R(赵久然), Wang X(王玺), Yi H-M(易红梅), Wang L(王璐), Guo J-L(郭景伦), Kuang M(匡猛), Sun Y-M(孙艳美). Redesigning and optimizing SSR core primer pairs for establishing DNA fingerprinting profiles. Mol Plant Breed (分子植物育种), 2007, 5(3): 443–447 (in Chinese with English abstract) |
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