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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1060-1068.doi: 10.3724/SP.J.1006.2013.01060

• 耕作栽培·生理生化 • 上一篇    下一篇

高温胁迫对水稻光合PSII系统伤害及其与叶绿体D1蛋白间关系

杨卫丽1,黄福灯2,曹珍珍1,雷炳婷1,胡东维1,程方民1,*   

  1. 1 浙江大学农业与生物技术学院,浙江杭州310058;2 浙江省农业科学院作物与核技术利用研究所,浙江杭州310031
  • 收稿日期:2012-12-04 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 通讯作者: 程方民, E-mail: chengfm@zju.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31271655, 31071366), 浙江省重大科技专项(2010C12003)和浙江省自然科学基金项目(LY12C13005)资助。

Effects of High Temperature Stress on PSII Function and Its Relation to D1 Protein in Chloroplast Thylakoid in Rice Flag Leaves

YANG Wei-Li1,HUANG Fu-Deng2,CAO Zhen-Zhen1,LEI Bing-Ting1,HU Dong-Wei1,CHENG Fang-Min1,*   

  1. 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Institute of Crop and Nucleus Technology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310031, China
  • Received:2012-12-04 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-22
  • Contact: 程方民, E-mail: chengfm@zju.edu.cn

摘要:

以水稻结实期的人工控温试验测定不同温度处理下水稻旗叶光合速率、叶绿素荧光参数的动态变化,并结合Western印迹与胶体金标记技术对叶肉细胞类囊体膜中D1蛋白的表达检测与活性定位,探讨了高温胁迫对D1蛋白存在形态与活性分布的影响,以及D1蛋白表达与叶片光合速率、PSII荧光参数的联系。结果表明,高温处理下叶片净光合速率下降、PSII潜在活性(Fv/Fo)PSII光能转化效率(Fv/Fm)降低,着高温胁迫时间的持续和叶片功能的衰退,类囊体膜结构损伤越严重,光能转化效率越低;在D1蛋白的两类存在形态中,非磷酸化D1蛋白和磷酸化D1蛋白在高温胁迫下的表达量均有所下降,但前者下降更明显;高温处理下控制D1蛋白表达的叶绿体psbA基因在转录水平呈下调表达,使D1蛋白合成及周转过程受到抑制,进而类囊体膜PSII反应中心的功能受损与叶绿体光合效率下降,揭示高温胁迫对叶片PSII系统的伤害受D1蛋白磷酸化过程和psbA基因表达变化的共同作用,进而影响不同水稻品种在高温胁迫下的光合速率和耐热性。

关键词: 水稻, 高温胁迫, PSII光合系统, D1蛋白, 蛋白印迹, 免疫定位

Abstract:

On the basis of the artificial controlled temperature treatments in growth chambers, influences of high temperature stress at rice filling stage on the expression and distribution of D1 protein in leaf chloroplast thylakoid, as well as their relations to the decline of leaf photosynthetic rate and the damage of PSII membrane system were investigated by detecting the temporal pattern of leaf photosynthetic rate and chlorophyll fluorescence parameters, with SDS-PAGE electrophoresis, western-blotting and immunogold labeling methods. The results showed that rice flag leaves exposed high temperature generally had lower net photosynthetic rate (Pn), PSII potential efficiency(Fv/Fo) and solar energy transmitting efficiency(Fv/Fm) compared with those under normal temperature, with more severe damage and remarkable function dropping in chloroplast thylakoid due to prolonging stress times and accelerating leaves senescence.The amount of D1 protein in rice leaves under high temperature stress decreased significantly. In the two types of D1 protein, the amount of non-phosphorylated D1 protein was more sensitive to high temperature stress than the phosphorylated D1 protein.The RNA transcription of psbA gene in chloroplast, controlling D1 protein synthesis, was down-regulated by high temperature stress, inhabiting mRNA transcription and protein translation in D1 protein synthesis, thereby causing the functional damage of PSII reaction centre in thylakoid and dropping leaf photosynthetic rate under high temperature stress. It could be suggested that the functional damage of PSII reaction centre under high temperature stress be caused by the combined action of D1 protein hosphorylation and psbA gene expression, resulting in the decrease of photosynthetic rate and high temperature tolerance in two rice genotypes.

Key words: Rice (Oryza sativa L.), High temperature stress, Photosynthetic system PSII, D1 protein, Western-blotting, Immunogold

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