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作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1808-1818.doi: 10.3724/SP.J.1006.2014.01808

• 耕作栽培·生理生化 • 上一篇    下一篇

夏玉米不同品种各阶段氮素效应综合评价方法

王丽君,李芸,王存凯,陶洪斌,王璞,廖树华   

  1. 中国农业大学农学与生物技术学院,北京 100193
  • 收稿日期:2014-02-20 修回日期:2014-07-06 出版日期:2014-10-12 网络出版日期:2014-08-04
  • 通讯作者: 廖树华, E-mail: sergzzl@cau.edu.cn
  • 基金资助:

    本研究由国家“十二五”粮食丰产科技工程项目(2011BAD16B10, 2012BAD04B02)和国家公益性行业(农业)科研专项(201203031)资助。

Comprehensive Evaluation Method for Effects of Nitrogen on Summer Maize Varieties at Different Stages

WANG Li-Jun,LI Yun,WANG Cun-Kai,TAO Hong-Bin,WANG Pu,LIAO Shu-Hua*   

  1. College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
  • Received:2014-02-20 Revised:2014-07-06 Published:2014-10-12 Published online:2014-08-04
  • Contact: 廖树华, E-mail: sergzzl@cau.edu.cn

摘要:

了解作物养分效应机制是合理施肥的重要理论基础。本文以浚单20、农华101和中农大43个玉米栽培品种为材料,综合品种遗传特征、光温及群体大小3个主要影响植株氮素效应的因素,以BLUP方法为基础,建立玉米品种间、不同器官在各生育阶段的氮素效应综合评价方法。结果表明,氮素效应评价模型中,各阶段日平均干物质积累量的理论值与实测值的相关性r值分别为0.9880.8810.9730.929;用独立试验样本对氮素效应评价模型检验,其配对t检验的P值均大于0.05,差异不显著;不同生育阶段、不同玉米品种氮素效应参数值有较大差异;氮素固定效应值在不同生育阶段、同一阶段的不同氮素构成中均表现出较大差异。综上,利用本文改进后的BLUP方法进行氮素效应评价及特征分析可以深入阐释不同玉米品种在各生长阶段积累的氮素对干物质生产的作用及影响,并进一步明确玉米氮素效应的共性特征、品种间的遗传差异、光温及群体影响机制等生物学规律。

关键词: 玉米, 氮素效应, 温度效应, 品种效应, 生育时期

Abstract:

Understanding nitrogen effects at various maize growing stages will benefit to fertilization regime recommendation and thus improve grain yield. Three maize varieties, Xundan 20, Nonghua 101, and Zhongnongda 4, were cultivated with different combinations of plant density and fertilizer levels in the experiment. During the growth period, daily temperature was recorded and leaf, stem, bract, spike-stalk and grain were sampled for the analysis of nitrogen concentration. Considering the three main factors which are genetic characteristics, environment impacts (light and temperature) and population differences affecting nitrogen effect comprehensively, the modified BLUP (Best Linear Unbiased Prediction) method was used to evaluate and analyse the nitrogen effect on different maize varieties and different organs at various growing stages. The results showed that correlation coefficients (r) between simulated daily dry matter accumulation and observed data were ranged from 0.88 to 0.99. Comparing independent sample to test evaluation model, paired T-test analysis showed no significant difference at P>0.05. The fitting effect was good in this model. For different growth stages and different maize varieties, there were significant differences for the parameters of nitrogen effect. The fixed effect of nitrogen showed significant difference at various growth stages and in different nitrogen constituents at the same stage. In conclusion, modified BLUP method can be well used to evaluate and analyse characteristics of the nitrogen effect, which can explain the function and influence of nitrogen accumulation on dry matter production at each growth stage of different maize varieties. It can further illustrate the biological law in maize, such as the common characteristics of nitrogen effect, the genetic differences between the varieties and the influence mechanism of sunlight, temperature and population.

Key words: Maize, Nitrogen effect, Temperature effect, Varieties effect, Growth stage

[1] 赵斌, 董树亭, 张吉旺, 刘鹏. 控释肥对夏玉米产量和氮素积累与分配的影响. 作物学报, 2010, 36: 1760–1768

Zhao B, Dong S T, Zhang J W, Liu P. Effects of controlled–release fertilizer on yield and nitrogen accumulation and distribution in summer maize. Acta Agron Sin, 2010, 36: 1760–1768 (in Chinese with English abstract)

[2] Raun W R, Johnson G V. Improving nitrogen use efficiency for cereal production. Agron J, 1999, 91: 357–363

[3] Wiesler F, Horst W J. Differences between maize cultivars in yield formation, nitrogen uptake and associated depletion of soil nitrate. J Agron Crop Sci, 1992, 168: 226–237

[4] 王相平, 黄冠华, 于利鹏, 黄权中. 土壤水氮迁移转化与作物生长耦合模拟. 农业工程学报, 2011, 27(3): 19–25

Wang X P, Huang G H, Yu L P, Huang Q Z. Coupled simulation on soil-water-nitrogen transport and transformation and crop growth. Trans CSAE, 2011, 27(3): 19–25 (in Chinese with English abstract)

[5] 王激清, 马文奇, 江荣风, 张福锁. 中国农田生态系统氮素平衡模型的建立及其应用. 农业工程学报, 2007, 23(8): 210–215

Wang J Q, Ma W Q, Jiang R F, Zhang F S. Development and application of nitrogen balance model of agro-ecosystem in China. Trans CSAE, 2007, 23(8): 210–215 (in Chinese with English abstract)

[6] 袁念念, 黄介生, 黄志强, 谢华, 吴谋松. 控制排水和施氮量对旱地土壤氮素运移转化的影响. 农业工程学报, 2012, 28(13): 106–112

Yuan N N, Huang J S, Huang Z Q, Xie H, Wu M S. Effects of controlled drainage and nitrogen fertilizer application on nitrogen migration and transformation in dryland. Trans CSAE, 2012, 28(13): 106–112 (in Chinese with English abstract)

[7] 李青军, 张炎, 胡伟, 孟凤轩, 冯广平, 胡国智, 刘新兰. 氮素运筹对玉米干物质积累、氮素吸收分配及产量的影响. 植物营养与肥料学报, 2011, 17(3): 755–760

Li Q J, Zhang Y, Hu W, Meng F X, Feng G P, Hu G Z, Liu X L. Effect of nitrogen management on maize dry matter accumulation, nitrogen uptake and distribution and maize yield. Plant Nutr Fert Sci, 2011, 17: 755–760 (in Chinese with English abstract)

[8] 宇万太, 张璐, 马强, 殷秀岩, 沈善敏. 施肥进步在粮食增产中的贡献及其地理分异. 应用生态学报, 2003, 14: 1855–1858

Yu W T, Zhang L, Ma Q, Yin X Y, Shen S M. Contribution of fertilization development to yield production and its geographic differentiation. Chin J Appl Ecol, 2003, 14(11): 1855–1858 (in Chinese with English abstract)

[9] 关义新, 林葆, 凌碧莹. 光、氮及其互作对玉米幼苗叶片光合和碳、氮代谢的影响. 作物学报, 2000, 26: 806–812

Guan Y X, Lin B, Lin B Y. The interactive effects of growth light condition and nitrogen supply on maize (Zea mays L.) seedling photosynthetic traits and metabolism of carbon and nitrogen. Acta Agron Sin, 2000, 26: 806–812 (in Chinese with English abstract)

[10] 王贺, 白由路, 杨俐苹, 卢艳丽, 王磊. 利用有效积温建立夏玉米追肥时期决策模型. 中国生态农业学报, 2012, 20: 408–413

Wang H, Bai Y L, Yang L P, Lu Y L, Wang L. A summer maize dressing decision–making model based on effective accumulated temperature. Chin J Eco-Agric, 2012, 20(4): 408–413 (in Chinese with English abstract)

[11] 卫晓轶, 李浩川, 谭晓军, 胡彦民, 汤继华, 刘宗华. 低氮胁迫对不同基因型玉米氮素吸收与分配动态的影响. 河南农业大学学报, 2012, 46: 353–358

Wei X Y, Li H C, Tan X J, Hu Y M, Tang J H, Liu J H. Dynamic variation of nitrogen absorption and distribution of different genotypes in maize under low nitrogen stress. J Henan Agric Univ, 2012, 46: 353–358 (in Chinese with English abstract)

[12] 黄高宝, 张恩和, 胡恒觉. 不同玉米品种氮素营养效率差异的生态生理机制. 植物营养与肥料学报, 2001, 7: 293–297

Huang G B, Zhang E H, Hu H J. Eco-physiological mechanism on nitrogen use efficiency difference of corn varieties. Plant Nutr Fert Sci, 2001, 7: 293–297 (in Chinese with English abstract)

[13] 王艳, 米国华, 陈范骏, 张福锁. 玉米氮素吸收的基因型差异及其与根系形态的相关性. 生态学报, 2003, 23: 297–302

Wang Y, Mi G H, Chen F J, Zhang F S. Genotypic differences in nitrogen uptake by maize inbred lines its relation to root morphology. Acta Ecol Sin, 2003, 23: 297–302 (in Chinese with English abstract)

[14] Presterl T, Groh S, Landbeck M, Seitz G, Schmidt W, Geiger H H. Nitrogen uptake and utilization efficiency of European maize hybrids developed under conditions of low and high nitrogen input. Plant Breed, 2002, 121: 480–486

[15] Moll R H, Kamprath E J, Jackson W A. Analysis and interpretation of factors which contribute to efficiency of nitrogen utilization. Agron J, 1982, 74: 562–564

[16] Plénet D, Lemaire G. Relationships between dynamics of nitrogen uptake and dry matter accumulation in maize crops. Determination of critical N concentration. Plant Soil, 1999, 216: 65–82

[17] 余佳. 春玉米施肥优化的数学模型分析. 安徽农业科学, 2012, 40: 5895–5897

Yu J. Mathematical model analysis on spring corn fertilizer optimization. J Anhui Agric Sci, 2012, 40: 5895–5897 (in Chinese with English abstract)

[18] 张经廷, 刘云鹏, 李旭辉, 梁效贵, 周丽丽, 周顺利. 夏玉米各器官氮素积累与分配动态及其对氮肥的响应. 作物学报, 2013, 39: 506–514

Zhang J T, Liu Y P, Li X H, Liang X G, Zhou L L, Zhou S L. Dynamic responses of nitrogen accumulation and remobilization in summer maize organs to nitrogen fertilizer. Acta Agron Sin, 2013, 39: 506–514 (in Chinese with English abstract)

[19] 杨国航, 崔彦宏, 刘树欣. 供氮时期对玉米干物质积累、分配和转移的影响. 玉米科学, 2004, 12(suppl-2): 104–106

Yang G H, Cui Y H, Liu S X. Effect of different stages of fertilizing nitrogen on accumulation, distribution and transfer of maize dry matter. J Maize Sci, 2004, 12(suppl-2): 104–106 (in Chinese with English abstract)

[20] 周丽丽, 冯汉宇, 阎忠敏, 刘克, 周顺利. 玉米叶片氮含量的高光谱估算及其品种差异. 农业工程学报, 2010, 26(8): 195–199

Zhou L L, Feng H Y, Yan Z M, Liu K, Zhou S L. Hyperspectral diagnosis of leaf N concentration of maize and variety difference. Trans CSAE, 2010, 26(8): 195–199 (in Chinese with English abstract)

[21] 郑志芳, 赵姣, 姜兴芳, 周顺利, 王璞, 廖树华. 玉米不同生育阶段养分效应评价方法研究. 中国生态农业学报, 2013, 21: 1064–1072

Zheng Z F, Zhao J, Jiang X F, Zhou S L, Wang P, Liao S H. Study on nutrient effect evaluation in different growth stages of maize. Chin J Eco-Agric, 2013, 21: 1064–1072 (in Chinese with English abstract)

[22] Zhao Y, Gowda M, Liu W, Wiirschum T, Maurer H P, Longin F H, Ranc N, Reif J C. Accuracy of genomic selection in European maize elite breeding populations. Theor Appl Genet, 2012, 124: 769–776

[23] Mendes F F, Guimarães L J M, Souza J C, Guimarães P E O, Pacheco C A P, Machado J R A, Meirelles W F, Silva A R, Parentoni S N. Adaptability and stability of maize varieties using mixed model methodology. Crop Breed Appl Biotechnol, 2012, 12: 111–117

[24] Viana J M S, Sobreira F M, De Resende M D V, Faria V R. Multi-trait BLUP in half-sib selection of annual crops. Plant Breed, 2010, 129: 599–604

[25] 张群远. 作物品种区域试验的精确度和统计模型研究. 中国农业大学博士学位论文, 北京, 2001

Zhang Q Y. Types and Statistical Indexes of Precision in Regional Crop Trials. PhD Dissertation of China Agricultural University, Beijing, China, 2001 (in Chinese with English abstract)

[26] 王丽君, 郑志芳, 姜兴芳, 赵姣, 王璞, 廖树华. 不同气象条件对玉米养分含量的影响. 玉米科学, 2013, 21(6): 64–67

Wang L J, Zheng Z F, Jiang X F, Zhao J, Wang P, Liao S H. Influence of different meteorological conditions on the nutrient content of corn. J Maize Sci, 2013, 21(6): 64–67 (in Chinese with English abstract)

[27] 乔嘉, 朱金城, 赵姣, 郑志芳, 王璞, 廖树华. 基于Logistic模型的玉米干物质积累过程对产量影响研究. 中国农业大学学报, 2011, 16(5): 32–38

Qiao J, Zhu J C, Zhao J, Zheng Z F, Wang P, Liao S H. Study on the dry matter distribution model of maize leaf. J China Agric Univ, 2011, 16: 32–38 (in Chinese with English abstract)

[28] 徐祥玉, 张敏敏, 翟丙年, 李生秀, 张兴昌, 王朝辉. 夏玉米氮效率基因型差异研究. 植物营养与肥料学报, 2006, 12: 495–499

Xu X Y, Zhang M M, Zhai B N, Li S X, Zhang X C, Wang C H. Genotypic variation in nitrogen use efficiency in summer maize. Plant Nutr Fert Sci, 2006, 12: 495–499 (in Chinese with English abstract)

[29] 徐祥玉, 张敏敏, 翟丙年, 李世清, 李生秀. 不同夏玉米品种生育后期干物质及氮素积累分配的研究. 西北植物学报, 2006, 26: 772–777

Xu X Y, Zhang M M, Zhai B N, Li S Q, Li S X. Dry matter and nitrogen accumulation and partition of different summer corn varieties at the later growth stage. Acta Bot Boreal-Occident Sin, 2006, 26: 772–777 (in Chinese with English abstract)

[30] 张瑞珍, 张恩和, 孙长占. 不同基因型玉米品种氮素营养效率差异的研究. 吉林农业大学学报, 2003, 25: 183–186

Zhang R Z, Zhang E H, Sun C Z. Study different genotype maize variety on diversity of nitrogen nutrition efficiency. J Jilin Agric Univ, 2003, 25: 183–186 (in Chinese with English abstract)

[31] 黄高宝, 张恩和, 胡恒觉. 不同玉米品种氮素营养效率差异的生态生理机制. 植物营养与肥料学报, 2001, 7: 293–297

Huang G B, Zhang E H, Hu H J. Eco-physiological mechanism on nitrogen use efficiency different of corn varieties. Plant Nutr Fert Sci, 2001, 7: 293–297 (in Chinese with English abstract)

[32] 蔡焕杰, 张振华, 柴红敏. 冠层温度定量诊断覆膜作物水分状况试验研究. 灌溉排水, 2001, 20(1): 1–4

Cai H J, Zhang Z H, Chai H M. Crop Canopy Temperature as an index for assessing quantitatively water status of cotton and corn mulched with plastic film. Irrigation & Drainage, 2001, 20(1): 1–4 (in Chinese with English abstract)

[33] 苏荣瑞, 周守华, 耿一风, 万素琴, 黄永平, 朱建强. 江汉平原水稻关键生育期冠层温度环境响应模型研究. 湖北农业科学, 2010, 49: 2673–2677

Su R R, Zhou S H, Geng Y F, Wan S Q, Huang Y P, Zhu J Q. Relationship between canopy temperature of rice and meteorological factors at the at the flowering stage in Jianghan Plain. Hubei Agric Sci, 2010, 49: 2673–2677 (in Chinese with English abstract)

[34] 邓强辉, 潘晓华, 石庆华. 作物冠层温度的研究进展. 生态学杂志, 2009, 28: 1162–1165

Deng Q H, Pan X H, Shi Q H. Research advances on crop canopy temperature. China J Ecol, 2009, 28: 1162–1165 (in Chinese with English abstract)

[35] 段永红, 李本纲, 陶澍. 冬小麦田午时冠层温度与气温和地温的关系. 应用气象学报, 2003, 14: 356–362

Duan Y H, Li B G, Tao S. Relationship among wheat surface temperature, air temperature and surface ground temperature at noon in the fields. J Appl Meteorol Sci, 2003, 14: 356–362 (in Chinese with English abstract)

[36] 颜军, 杨德光. 4个玉米品种氮素利用效率的比较研究. 玉米科学, 2010, 18(2): 91–95

Yan J, Yang D G. Comparison on N utilization efficiency of four maize varieties. J Maize Sci, 2010, 18(2): 91–95 (in Chinese with English abstract)

[37] 李锦辉, 李潮海. 不同基因型玉米氮素利用的机理研究. 核农学报, 2007, 21: 173–176

Li J H, Li C H. Nitrogen utilization mechanism of different genotype maize. J Nucl Agric Sci, 2007, 21: 173–176 (in Chinese with English abstract)

[38] 王玲敏, 叶优良, 陈范骏, 尚云峰. 施氮对不同品种玉米产量、氮效率的影响. 中国生态农业学报, 2012, 20: 529–535

Wang L M, Ye Y L, Chen F J, Shang Y F. Effect of nitrogen fertilization on maize yield and nitrogen efficiency of different maize varieties. Chin J Eco-Agric, 2012, 20: 529–535 (in Chinese with English abstract)

[39] 黄冬维, 张元跃, 谭伟明. BLUP法在畜禽育种中的应用. 中国畜禽种业, 2008, 4(8): 66–68

Huang D W, Zhang Y Y, Tan W M. Application of the BLUP method in animal breeding. Chin Livestock Poultry Breed, 2008, 4(8): 66–68 (in Chinese)

[40] 常英新, 王晓霞. 应用动物模型BLUP估计猪育种值的简介. 北京农学院学报, 2000, 15(1): 81–86

Chang Y X, Wang X X. An introduction on predicting breeding value of swine applying BLUP-animal model. J Beijing Agric Coll, 2000, 15(1): 81–86 (in Chinese)

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