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作物学报 ›› 2016, Vol. 42 ›› Issue (04): 492-500.doi: 10.3724/SP.J.1006.2016.00492

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

藜麦EST-SSR的开发及通用性分析

张体付1,**,戚维聪1,**,顾闽峰2,张晓林1,李坦1,赵涵1,*   

  1. 1 江苏省农业科学院 / 江苏省农业生物学重点实验室,江苏南京 210014;2 盐城市新洋农业试验站,江苏盐城 224336
  • 收稿日期:2015-05-31 修回日期:2016-01-11 出版日期:2016-04-12 网络出版日期:2016-01-25
  • 通讯作者: 赵涵, E-mail: zhaohan@jaas.ac.cn, Tel: 025-84390751
  • 基金资助:

    本研究由江苏省农业科技自主创新资金项目(CX(14)2044), 美国国际发展署Securing water for food award-salt tolerant quinoa子项目(UR4443 B)资助。

Exploration and Transferability Evaluation of EST-SSRs in Quinoa

ZHANG Ti-Fu1,**,QI Wi-Cong1,**,GU Min-Feng2,ZHANG Xiao-Lin1,LI Tan1,ZHAO Han1,*   

  1. 1 Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2 Xinyang Agricultural Experiment Station of Yancheng City, Yancheng 224336, China
  • Received:2015-05-31 Revised:2016-01-11 Published:2016-04-12 Published online:2016-01-25
  • Contact: 赵涵, E-mail: zhaohan@jaas.ac.cn, Tel: 025-84390751
  • Supported by:

    This study was supported by Jiangsu Agriculture Science and Technology Innovation Fund (CX(14)2044) and the subproject of “Securing water for food award-salt tolerant quinoa” from USAID (UR4443 B).

摘要:

藜麦因营养均衡受到越来越多的关注,但尚未深入开展其基础研究。开发微卫星序列重复SSR分子标记将为藜麦的遗传分析提供重要资源。本研究利用NCBI数据库中藜麦RNA测序RNA-Seq及表达序列标签EST数据挖掘、验证及评价藜麦EST-SSR,共发现1862个藜麦非单核苷酸EST-SSR。其中,二核苷酸重复最多(38.3%),六核苷酸重复最少(11.7%)。不同重复类型SSR的数量随着核苷酸数目的增加呈下降趋势。在随机选取验证的119个EST-SSR标记中,66 (55.9%)个能够扩增出清晰条带,39个在4份藜麦资源中具有多态性,且其多态性与重复序列长度不具有显著相关性。t测验显示,多态性EST-SSR在藜麦与其他藜科种质间不存在显著差异,说明其具有良好的通用性,可用于藜科物种的遗传关系分析。

关键词: 藜麦, EST-SSR, 分子标记, 遗传关系, 通用性

Abstract:

Quinoa draws more and more attentions from people, since quinoa seed as grain has comprehensive nutrients.But the fundamental research on quinoa just starts and remains in a moderated level. Simple sequence repeat (SSR) development in quinoa will enhance the resource for its genetic analysis. Here,the available RNA based sequencing (RNA-Seq) and expressed sequence tag (EST) data of quinoa deposited in the National Center for Biotechnology Information (NCBI) were engaged in EST-SSR development.Totally,1862 non-mononucleotide EST-SSRswere identified. Among the EST-SSRs, the dinucleotide type was the most abundant (38.3%), and the hexanucleotidewas the minimal(11.7%). The amount of the EST-SSR showed the declined trend along with the increase in its motif nucleotide length. Among 119 EST-SSR primersrandomly chosen for validation,66(55.9%) primers could give clearamplification bandsand 39 showed polymorphismsinfour quinoa accessions.Further analysis showed that the polymorphisms of EST-SSRs had no significant correlation with their motif nucleotide length. In addition, t-test demonstrated that the significant difference of EST-SSRpolymorphismswas not occurredbetween quinoa accessions and otherChenopodiaceae germplasm. These results indicating EST-SSRs developed in quinoa could be transferable in Chenopodiaceous genus and applied in genetic relationship analysis.

Key words: Quinoa, EST-SSR, Molecular marker, Genetic relationship, Transferability

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