作物学报 ›› 2016, Vol. 42 ›› Issue (11): 1656-1665.doi: 10.3724/SP.J.1006.2016.01656
夏雪,马晨雨,白青河,冯园园,王顺友,王喜,周青利,席章营*
XIA Xue, MA Chen-Yu, BAI Qing-He, FENG Yuan-Yuan, WANG Shun-You, WANG Xi, ZHOU Qing-Li, and XI Zhang-Ying*
摘要:
玉米籽粒发育及产量是玉米重要的经济性状。本研究以玉米骨干自交系郑58为试材,采用同源克隆法得到一个与玉米籽粒发育相关的基因ZmMADS-RIN。该基因的cDNA全长859 bp,开放阅读框为768 bp,编码255个氨基酸,与玉米B73中所对应的cDNA的编码区序列相比,共有6个SNPs位点的差异,3个氨基酸残基发生了变化。生物信息学分析表明,ZmMADS-RIN蛋白的相对分子量为29.23 kD,理论等电点(pI)为8.84,是一种亲水性蛋白,不含信号肽序列,也不含跨膜结构;具有5个磷酸化位点;二级结构中含有50.20%的α-螺旋(alpha helix)、27.45%的无规则卷曲(random coil)、14.51%的延伸链(extended strand)和7.84%的β-转角(beta turn);该蛋白位于细胞核内;其氨基酸序列中含有高度保守的MADS结构域、相对保守的K结构域、低保守的I结构域和最不稳定的C结构域,是典型的MIKC型MADS-box蛋白;系统进化树分析表明,ZmMADS-RIN蛋白属于AGL6分枝,与水稻基因OsMADS6的相似性最高,为89%。ZmMADS-RIN基因在玉米自交系郑58的籽粒中特异表达,在根及不同时期的叶片中没有检测到表达信号。荧光定量PCR结果显示,ZmMADS-RIN基因的表达量在玉米籽粒发育的不同阶段呈一定规律的变化,在授粉后0~20 d,呈上调表达趋势,授粉后25 d表达量迅速下降至较低水平,而在授粉后30~40 d则检测不到其表达。推测该基因可能与玉米籽粒的发育有关。
| [1]NgM,YanofskyMF.FunctionandevolutionoftheplantMADS-boxgenefamily.NatRevGenet,2001,2:186–195 [2]Alvarez-BuyllaER,LiljegrenSJ,PelazS,GoldSE,BurgeffC,DittaGS,Vergara-SilvaF,YanofskyMF.MADS-boxgeneevolutionbeyondflowers:expressioninpollen,endosperm,guardcells,rootsandtrichomes.PlantJ,2000,24:457–466 [3]deBodtS,RaesJ,FlorquinK,RombautsS,RouzéP,Thei?enG,VandePeerY.GenomewidestructuralannotationandevolutionaryanalysisofthetypeIMADS-boxgenesinplants.JMolEvol,2003,56:573–586 [4]ZhangZB,LiHY,ZhangDF,LiuYH,FuJ,ShiYS,SongYC,WangTY,LiY.CharacterizationandexpressionanalysisofsixMADS-boxgenesinmaize(ZeamaysL.).JPlantPhysiol,2012,169:797–806 [5]LesebergCH,EisslerCL,WangX,JohnsMA,DuvallMR,MaoL.InteractionstudyofMADS-domainproteinsintomato.JExpBot,2008,59:2253–2265 [6]ShuYJ,YuDS,WangD,GuoDL,GuoCH.Genome-widesurveyandexpressionanalysisoftheMADS-boxgenefamilyinsoybean.MolBiolRep,2013,40:3901–3911 [7]BeckerA,Thei?enG.ThemajorcladesofMADS-boxgenesandtheirroleinthedevelopmentandevolutionoffloweringplants.MolPhylogenetEvol,2003,29:464–489 [8]KuAT,HuangYS,WangYS,MaDF,YehKW.IbMADS1(IpomoeabatatasMADS-box1gene)isinvolvedintuberousrootinitiationinsweetpotato(Ipomoeabatatas).AnnBot,2008,102:57–67 [9]BenllochR,RoqueE,FerrándizC,CossonV,CaballeroT,PenmetsaRV,BeltránJP,Ca?asLA,RatetP,Madue?oF.AnalysisofBfunctioninlegumes:PISTILLATAproteinsdonotrequirethePImotifforfloralorgandevelopmentinMedicagotruncatula.PlantJ,2009,60:102–111 [10]FerrarioS,ShchennikovaAV,FrankenJ,ImminkRGH,AngenentGC.ControloffloralmeristemdeterminacyinpetuniabyMADS-boxtranscriptionfactors.PlantPhysiol,2006,140:890–898 [11]韩利涛,姜伟,杨守萍,喻德跃,盖钧镒.大豆细胞质雄性不育系MADS-box基因的分离分析.作物学报,2010,36:905–910 HanLT,JiangW,YangSP,YuDY,GaiJY.IsolationandanalysisofMADS-boxgenefromsoybean(GlycinemaxL.Merr)cytoplasmicmalesterileline.ActaAgronSin,2010,36:905–910(inChinesewithEnglishabstract) [12]KaufmannK,Mui?oJM,JaureguiR,AiroldiCA,SmaczniakC,KrajewskiP,AngenentGC.TargetgenesoftheMADStranscriptionfactorSEPALLATA3:IntegrationofdevelopmentalandhormonalpathwaysintheArabidopsisflower.PLoSBiol,2009,7:854–875 [13]MichaelsSD,DittaG,Gustafson-BrownC,PelazS,YanofskyM,AmasinoRM.AGL24actsasapromoteroffloweringinArabidopsisandispositivelyregulatedbyvernalization.PlantJ,2003,33:867–874 [14]MaraCD,IrishVF.TwoGATAtranscriptionfactorsaredownstreameffectorsoffloralhomeoticgeneactioninArabidopsis.PlantPhysiol,2008,147:707–718 [15]汪潇琳,陈艳萍,喻德跃.MADS-box基因GmAGL15在大豆种子发育过程中的表达.作物学报,2008,34:330–332 WangXL,ChenYP,YuDY.ExpressionoftheMADS-boxgeneGmAGL15inseeddevelopmentofsoybean.ActaAgronSin,2008,34:330–332(inChinesewithEnglishabstract) [16]ItoY,KitagawaM,IhashiN,YabeK,KimbaraJ,YasudaJ,ItoH,InakumaT,HiroiS,KasumiT.DNA-bindingspecificity,transcriptionalactivationpotential,andtherinmutationeffectforthetomatofruit-ripeningregulatorRIN.PlantJ,2008,55:212–223 [17]VrebalovJ,RuezinskyD,PadmanabhanV,WhiteR,MedranoD,DrakeR,SchuchW,GiovannoniJ.AMADS-boxgenenecessaryforfruitripeningatthetomatoRipening-Inhibitor(Rin)locus.Science,2002,296:343–346 [18]BattagliaR,BrambillaV,ColomboL,StuitjeAR,KaterMM.FunctionalanalysisofMADS-boxgenescontrollingovuledevelopmentinArabidopsisusingtheethanol-induciblealcgene-expressionsystem.MechanismsDevelopment,2006,123:267–276 [19]MoyleR,FairbairnDJ,RipiJ,CroweM,BotellaJR.Developingpineapplefruithasasmalltranscriptomedominatedbymetallothionein.JExpBot,2005,56:101–112 [20]DreniL,JacchiaS,FornaraF,FornariM,OuwerkerkPBF,AnG,ColomboL,KaterMM.TheD-lineageMADS-boxgeneOsMADS13controlsovuleidentityinrice.PlantJ,2007,52:690–699 [21]MazzucatoA,OlimpieriI,SiligatoF,PicarellaME,SoressiGP.CharacterizationofgenescontrollingstamenidentityanddevelopmentinaparthenocarpictomatomutantindicatesarolefortheDEFICIENSorthologinthecontroloffruitset.PhysiolPlant,2008,132:526–537 [22]刘菊华,徐碧玉,张静,金志强.MADS-box转录因子的相互作用及对果实发育和成熟的调控.遗传,2010,32:893–902 LiuJH,XuBY,ZhangJ,JinZQ.TheinteractionofMADS-boxtranscriptionfactorsandmanipulatingfruitdevelopmentandripening.Hereditas,2010,32:893–902(inChinesewithEnglishabstract) [23]ColomboM,MasieroS,VanzulliS,LardelliP,KaterMM,ColomboL.AGL23,atypeIMADS-boxgenethatcontrolsfemalegametophyteandembryodevelopmentinArabidopsis.PlantJ,2008,54:1037–1048 [24]DinnenyJR,WeigelD,YanofskyMF.AgeneticframeworkforfruitpatterninginArabidopsisthaliana.Development,2005,132:4687–4696 [25]SeymourGB,RyderCD,CevikV,HammondJP,PopovichA,KingGJ,VrebalovJ,GiovannoniJJ,ManningK.ASEPALLATAgeneisinvolvedinthedevelopmentandripeningofstrawberry(Fragaria×ananassaDuch.)fruit,anonclimacterictissue.JExpBot,2011,62:1179–1188 [26]周厚成,李刚,赵霞,康兆茹,郭蔼光.森林草莓全基因组MADS-box转录因子基因的鉴定及凤梨草莓果实FaMADS1基因克隆.植物生理学报,2014,50:1630–1638 ZhouHC,LiG,ZhaoX,KangZR,GuoAG.Genome-widesequenceidentificationofMADS-boxtranscriptionfactorgenefamilyfromFragariavescaandcloningofFaMADS1genefromF.ananassafruit.PlantPhysiolJ,2014,50:1630–1638(inChinesewithEnglishabstract) [27]FerrándizC,LiljegrenSJ,YanofskyMF.NegativeregulationoftheSHATTERPROOFgenesbyFRUITFULLduringArabidopsisfruitdevelopment.Science,2000,289:436–438 [28]LiljegrenSJ,DittaGS,EshedY,SavidgeB,BowmanJL,YanofskyMF.SHATTERPROOFMADS-boxgenescontrolseeddispersalinArabidopsis.Nature,2000,404:766–770 [29]DongTT,HuZL,DengL,WangY,ZhuMK,ZhangJL,ChenGP.AtomatoMADS-boxtranscriptionfactor,SlMADS1,actsasanegativeregulatoroffruitripening.PlantPhysiol,2013,163:1026–1036 [30]MünsterT,DeleuW,WingenLU,OuzunovaM,CacharrónJ,FaiglW,WerthS,KimJTT,SaedlerH,Thei?enG.MaizeMADS-boxgenesgalore.Maydica,2002,47:287–301 [31]MalcomberST,KelloggEA.HeterogeneousexpressionpatternsandseparaterolesoftheSEPALLATAgeneLEAFYHULLSTERILE1ingrasses.PlantCell,2004,16:1692–1706 [32]ThompsonBE,BartlingL,WhippleC,HallDH,SakaiH,SchmidtR,HakeS.bearded-earencodesaMADSboxtranscriptionfactorcriticalformaizefloraldevelopment.PlantCell,2009,21:2578–2590 [33]Lopez-DeeZP,WittichP,PèME,RigolaD,BuonoID,GorlaMS,KaterMM,ColomboL.OsMADS13,anovelriceMADS-boxgeneexpressedduringovuledevelopment.DevGenet,1999,25:237–244 [34]MooreS,VrebalovJ,PaytonP,GiovannoniJ.Useofgenomicstoolstoisolatekeyripeninggenesanalysefruitmaturationintomato.JExpBot,2002,53:2023–2030 [35]DongTT,ChenGP,TianSB,XieQL,YinWC,ZhangYJ,HuZL.Anon-climactericfruitgeneCaMADS-RINregulatesfruitripeningandethylenebiosynthesisinclimactericfruit.PLoSOne,2014,9:e95559 [36]WangL,YinXJ,ChengCX,WangH,GuoRR,XuXZ,ZhaoJ,ZhengY,WangXP.EvolutionaryandexpressionanalysisofaMADS-boxgenesuperfamilyinvolvedinovuledevelopmentofseededandseedlessgrapevines.MolGenetGenomics,2015,290:825–846 [37]LiuJH,XuBY,HuLF,LiMY,SuW,WuJ,YangJH,JinZQ.InvolvementofabananaMADS-boxtranscriptionfactorgeneinethyleneinducedfruitripening.PlantCellRep,2009,28:103–111 [38]WangXT,WuLJ,ZhangSF,WuLC,KuLX,WeiXM,XieLL,ChenYH.RobustexpressionandassociationofZmCCA1withcircadian.PlantCellRep,2011,30:1261–1272 [39]RajeevanMS,RanamukhaarachiDG,VernonSD,UngerER.Useofreal-timequantitativePCRtovalidatetheresultsofcDNAarrayanddifferentialdisplayPCRtechnologies.Methods,2001,25:443–451 [40]ZhangJ,NallamilliBR,MujahidH,PengZH.OsMADS6playsanessentialroleinendospermnutrientaccumulationandissubjecttoepigeneticregulationinrice(Oryzasativa).PlantJ,2010,64:604–617 [41]LeeS,KimJ,SonJS,NamJ,JeongDH,LeeK,JangS,YooJ,LeeJ,LeeDY,KangHG,AnG.SystematicreversegeneticscreeningofT-DNAtaggedgenesinriceforfunctionalgenomicanalyses:MADS-boxgenesasatestcase.PlantCellPhysiol,2003,44:1403–1411 [42]BusiVM,BustamanteC,AngeloCD,Hidalgo-CuevasM,BoggioSB,ValleEM,ZabaletaE.MADS-boxgenesexpressedduringtomatoseedandfruitdevelopment.PlantMolBiol,2003,52:801–815 [43]张冬梅,刘洋,赵永锋,祝丽英,黄亚群,郭晋杰,陈景堂.不同杂种优势群玉米籽粒灌浆速率分析.中国农业科学,2014,47:3323–3335 ZhangDM,LiuY,ZhaoYF,ZhuLY,HuangYQ,GuoJJ,ChenJT.Analysisofmaizegrainfillingrateindifferentheteroticgroups.SciAgricSin,2014,47:3323–3335(inChinesewithEnglishabstract) |
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