作物学报 ›› 2018, Vol. 44 ›› Issue (01): 75-81.doi: 10.3724/SP.J.1006.2018.000075
罗云,马璇,谷俊辰,闫海芳*
LUO Yun, MA Xuan, GU Jun-Chen,YAN Hai-Fang*
摘要:
SIZ1是植物细胞蛋白质翻译后修饰SUMO化的E3连接酶,参与植物蛋白相互作用、定位和抗逆反应等。为研究BrSIZ1在津田芜菁中的表达特性,本研究克隆了津田芜菁SIZ1基因全长cDNA序列,命名为BrSIZ1 (GenBank登录号为KY441465),该基因全长2754 bp,其ORF全长2571 bp,编码856个氨基酸残基的多肽。构建了BrSIZ1-GFP表达载体进行亚细胞定位研究,结果显示BrSIZ1-GFP定位于细胞核内,可能在细胞核中发挥其功能。利用荧光定量PCR检测表明,该基因表达量在叶子中最高,幼苗和红色根皮中次之,表达具有组织特异性。而且BrSIZ1在芜菁根皮中的表达受长波紫外线(UV-A)诱导,在4°C、37°C胁迫的幼苗中,表达量增加。
[1] Girdwood D W H, Tatham M H, Hay R T. SUMO and transcriptional regulation. Semin Cell Biol, 2004, 15: 201–210 [2] Johnson E S. Proteion modification by SUMO. Annu Rev Biochem, 2004, 73: 355–382 [3] Novatchkova M, Budhiraja R. Coupland G. SUMO conjugation in plants, Planta, 2004, 220: 1–8 [4] Johnson E S, Gupta A A. An E3-like factor that promotes SUMO conjugation to the yeast septins. Cell, 2001, 106: 735–744 [5] Sharrocks A D. PIAS proteins and transcriptional regulation-more than just SUMO E3 ligases. Genes & Development, 2006, 20: 754–758 [6] Bienz M. The PHD finger, a nuclear protein-interaction domain. Trends in biochemical sciences, 2006, 31: 35–40 [7] Aravind L, Koon E V. SAP—a putative DNA-binding motif involved in chromosomal organization. Trend Biochem Sci, 2000, 25: 112–114 [8] Miura K, Jin J B, Hasegawa P M. Sumoylation, a post-translational regulatory process in plante. Curr Opin Plant Biol, 2007, 10: 495–502 [9] Huang L X, Yang S G, Zhang S C. The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root. Plant J, 2009, 60: 666–678 [10] Jin J B, Jin Y H, Lee J. The SUMO E3 ligase AtS1Z1 regulates flowering by controlling a salicylic acid-mediated floral promotion pathway and through affects on FLC chromatin structure. Plant J, 2008, 53: 530–540 [11] Thangasamy S, Guo C L, Chuang M H, Lai M H, Chen J, Jauh G Y. Rice SIZ1, a SUMO E3 ligase, controls spikelet fertility through regulation of anther dehiscence. New Phytologist, 2011, 189: 869–882 [12] Kurepa J, Walker J M, Smalle J. The small ubiquitin-like modifier (SUMO) protein modification system in Arabidopsis – Accumulation of SUMO1 and -2 conjugates is increased by stress. J Biol Chem, 2003, 278: 6862–6872 [13] Lee J Y, Nam J, Park H C, Na G. Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase. Plant J, 2007, 49: 79–90 [14] Miura K, Jin J B, Lee J, Yoo C Y, Stirm T, Ashworth E N, Bressan R A, Yun D J, Hasegawa P M. SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis.Plant Cell, 2007, 19: 1403–1414 [15] Yoo C Y, Miura K, Jin J B. SIZ1 (small ubiquitin-like modifi er) E3 ligase facilitates basal thermotolerance in Arabidopsis independent of salicylic acid. Plant Physiol, 2006, 142:1548–1558 [16] Catala R, Ouyang J, Abreu I A. The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses. Plant Cell, 2007, 19: 2952–2966 [17] Zhang S, Zhuang K, Wang S, Lv J, Ma N N, Meng Q W. A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco. JIPB, 2017, doi: 10.1111/jipb.12514 [18] Calderon-Villalobos L I, Nill C, Marrocco K. The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress. Gene, 2007, 392: 106–116 [19] Zhou B, Li Y, Xu Z, Yan H, Homma S, Kawabata S. Ultraviolet A-specific induction of anthocyanin biosynthesis in the swollen hypocotyls of turnip (Brassica rapa). J Exp Bot, 2007, 58: 1771–1781 [20] Kawabata S, Kusahara Y, Li Y, Sakiyama R. The regulation of anthocyanin biosynthesis in Eustoma grandiflorum under low light conditions. J Jpn Soc Hort Sci, 1999, 68: 519–526 [21] Frohman M A, Dush M K, Martin G R. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA, 1988, 85: 8998–9002 [22] Zhou B, Zhao X, Kawabata S, Li Y. Transient expression of a foreign gene by direct incorporation of DNA into intact plant tissue through vacuum infiltration. Biotechnol Lett, 2009, 31: 1811–1815 [23] Dingwall C, Robbins J, Dilworth S M, Roberts B, Richardson W D. The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen. J Cell Biol, 1988, 107: 841–849 [24] Liu F, Wang X, Su M Y, Yu M Y, Zhang S C, Lai J B, Yang C W, Wang YQ. Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium. BMC Plant Biology, 2015, 15: 225–239 [25] Huang X, Ouyang X, Deng X W. Beyond repression of photomorphogenesis: role switching of COP/DET/FUS in light signaling. Curr Opin Plant Biol, 2014, 22: 96–103 [26] Lay H A, Sudip C, Ning W, Tokitaka O, Alfred B, Deng X W. Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development. Mol Cell, 1998, 1: 213–222 |
[1] | 崔连花, 詹为民, 杨陆浩, 王少瓷, 马文奇, 姜良良, 张艳培, 杨建平, 杨青华. 2个玉米ZmCOP1基因的克隆及其转录丰度对不同光质处理的响应[J]. 作物学报, 2022, 48(6): 1312-1324. |
[2] | 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371. |
[3] | 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400. |
[4] | 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102. |
[5] | 周慧文, 丘立杭, 黄杏, 李强, 陈荣发, 范业赓, 罗含敏, 闫海锋, 翁梦苓, 周忠凤, 吴建明. 甘蔗赤霉素氧化酶基因ScGA20ox1的克隆及功能分析[J]. 作物学报, 2022, 48(4): 1017-1026. |
[6] | 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800. |
[7] | 晋敏姗, 曲瑞芳, 李红英, 韩彦卿, 马芳芳, 韩渊怀, 邢国芳. 谷子糖转运蛋白基因SiSTPs的鉴定及其参与谷子抗逆胁迫响应的研究[J]. 作物学报, 2022, 48(4): 825-839. |
[8] | 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895. |
[9] | 冯亚, 朱熙, 罗红玉, 李世贵, 张宁, 司怀军. 马铃薯StMAPK4响应低温胁迫的功能解析[J]. 作物学报, 2022, 48(4): 896-907. |
[10] | 靳容, 蒋薇, 刘明, 赵鹏, 张强强, 李铁鑫, 王丹凤, 范文静, 张爱君, 唐忠厚. 甘薯Dof基因家族挖掘及表达分析[J]. 作物学报, 2022, 48(3): 608-623. |
[11] | 张艳波, 王袁, 冯甘雨, 段慧蓉, 刘海英. 棉籽油分和3种主要脂肪酸含量QTL分析[J]. 作物学报, 2022, 48(2): 380-395. |
[12] | 谢琴琴, 左同鸿, 胡燈科, 刘倩莹, 张以忠, 张贺翠, 曾文艺, 袁崇墨, 朱利泉. 甘蓝自交不亲和相关基因BoPUB9的克隆及表达分析[J]. 作物学报, 2022, 48(1): 108-120. |
[13] | 张波, 裴瑞琴, 杨维丰, 朱海涛, 刘桂富, 张桂权, 王少奎. 利用单片段代换系鉴定巴西陆稻IAPAR9中的粒型基因[J]. 作物学报, 2021, 47(8): 1472-1480. |
[14] | 尹明, 杨大为, 唐慧娟, 潘根, 李德芳, 赵立宁, 黄思齐. 大麻GRAS转录因子家族的全基因组鉴定及镉胁迫下表达分析[J]. 作物学报, 2021, 47(6): 1054-1069. |
[15] | 许静, 潘丽娟, 李昊远, 王通, 陈娜, 陈明娜, 王冕, 禹山林, 侯艳华, 迟晓元. 花生油脂合成相关基因的表达谱分析[J]. 作物学报, 2021, 47(6): 1124-1137. |
|