关键词: 普通菜豆, 菜豆炭疽病, 抗病基因, 分子标记, SCAR

Abstract:

Bean Anthracnose is one of the main diseases, which causes 20%–30% reduction of output in common bean. Based on the evaluated results of disease resistance to anthracnose with No. 81 physiological race from 1986 to 2000, 127 accessions of resistant and high resistant germplasm resources were selected to be used in this study. In order to identify different types of resistance genes in these materials, we assessed the reliability and effectiveness of 7 SCAR primers（SBB14_1150/1050, SAS13₉₅₀, SCAreoli₁₀₀₀, SH18₁₁₀₀, SAB3₄₀₀, SB12₃₅₀, and SCF10¹⁰⁷²）which linked with resistant anthracnose genes. 12 identification host varieties of common bean were used as control. The results showed that SBB14_1150/1050 had no differential amplification and SAS13₉₅₀ had no target amplification. The further identification of anthracnose resistant genes was conducted by using 5 SCAR primers in 127 accessions of common bean. There were no SCAR markers detected in 82 accessions. 1–3 SCAR markers appeared in 45 accessions respectively. SCAreoli₁₀₀₀ (Co-2) marker appeared in 13, SH181100 (Co-42) marker in 13, SAB3₄₀₀ (Co-5) marker in 5, SB12₃₅₀ (Co-9) marker in 9, SCF10₁₀₇₂ (Co-10) marker in 11 accessions of common bean. The results also revealed that there were 5 accessions with broad-spectrum resistance to anthracnose and there was a correlation between the resistant genes and origin of the germplasm resource. This study provided an important genetic information for breeding program on disease resistance in common bean.

Key words: Common bean, Anthracnose, Resistance gene, Molecular marker, SCAR