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作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1236-1243.doi: 10.3724/SP.J.1006.2009.01236

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于大豆胞囊线虫抗病候选基因rhg1的InDe1标记发掘与鉴定

南海洋,李英慧**,常汝镇,邱丽娟*   

  1. 中国农业科学院作物科学研究所/高家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源利用重点开放实验室，北京100081
  • 收稿日期:2008-10-28 修回日期:2009-03-16 出版日期:2009-07-12 网络出版日期:2009-05-19
  • 通讯作者: 邱丽娟, E-mail: qiu_lijuan@263.net
  • 作者简介:E-mail: haiyang_mei@163.com
  • 基金资助:

    本研究由国家自然科学基金项目（30490251和30471096），高家高技术研究发展计划（863计划）项目（2006AA100104，2006AA10Z164，2006AA10Z1B3)，高家“十一五”科技支撑计划项目（2006BAD13B05），国际科技合作项目（2008DFA30550），中国农业科学院作物科学研究所科研基金资助。

Development and Identification of InDel Marker Based on rhg1 Gene for Resistance to Soybean Cyst Nematode(Heterodera glycines Ichinohe)

NAN Hai-Yang,LI Ying-Hui**,CHANG Ru-Zhen,QIU Li-Juan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Germplasm & Biotechnology/Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，Beijing 100081，China
  • Received:2008-10-28 Revised:2009-03-16 Published:2009-07-12 Published online:2009-05-19
  • Contact: QIU Li-Juan, E-mail: qiu_lijuan@263.net
  • About author:E-mail: haiyang_mei@163.com

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被引次数

15

摘要：

大豆胞囊线虫病是严重危害大豆生产的重要病害之一，根据抗病候选基因发掘标记可以为分子标记辅助选择抗病材料提供标记资源。本研究通过对大豆胞囊线虫抗病候选基因rhg1的序列比对分析，发现4个插入/删除位点，针对其中3个多碱基插入/缺失位点开发了InDel标记。应用开发的3个InDel标记对33份栽培大豆进行基因型鉴定，共检测到等位变异11个，平均每个位点3.67个。其中rhg1-I1位点有等位变异5个，rhg1-I2位点有等位变异2个；rhg1-I4位点有等位变异4个。各等位变异发生频率范围为0.8%~77.3%。InDel标记与大豆胞囊线虫抗性间的关联分析表明，rhg1-I4为抗性相关标记，对抗病资源的检出效率为88.2%，对感病资源的检出效率为100%。该标记的288 bp等位变异和294 bp等位变异为抗病相关等位变异，269 bp等位变异和272 bp等位变异为感病相关等位变异。此标记与常用于标记辅助选择的Satt309配合鉴定可以提高SCN抗病资源的检测效率。

关键词: 大豆, 胞囊线虫病, InDel标记

Abstract:

Soybean cyst nematode (Heterodera glycaines, SCN) is one of the most important pests of soybean in the world. New molecular markers developed from resistance genes can provide marker resources for MAS (marker-assisted selection) breeding. Among four InDel loci detectedby comparing thesequences of candidate rhg1 conferringSCN resistance, three multiple-bases InDel loci were used to develop markers. A total of 11 alleles were detected in 33 cultivated soybean germplasm using three InDel markers developed. There were 5 alleles at rhg1-I1 locus, 2 and 4 at rhg1-I2 and rhg1-I4 respectively. Allelic variations at three InDel markers were assayed in the genotypes and discovered that rhg1-I4 marker was related to SCN resistance, which could distinguish 88.2% of resistant germplasm and 100% of the susceptible germplasm. Alleles of 288 and 294 bp presented in the SCN-resistant germplasm, and alleles 269 and 272 bp presented in the SCN-susceptible germplasm. In addition, rhg1-I4 could be used in MAS for the progenies of cross between typical southern US cultivar Lee crossed and the important resistant source PI88788. Combing with Satt309 which displayed the identical allele on these genotypes, rhg1-I4 would be higher effective in identifying germplasm with rhg1 conferring resistance in the MAS breeding.

Key words: Soybean, SCN, InDel marker

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