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作物学报 ›› 2009, Vol. 35 ›› Issue (8): 1558-1561.doi: 10.3724/SP.J.1006.2009.01558

• 研究简报 • 上一篇    下一篇

cpt1基因与水稻根负向光性的关系

汪月霞1,2,王忠2,*,刘全军1,赵会杰1,顾蕴洁2,钱晓旦2,袁志良1   

  1. 1河南农业大学生命科学学院，河南郑州450002；2扬州大学生物科学与技术学院，江苏扬州225009
  • 收稿日期:2008-11-15 修回日期:2009-02-17 出版日期:2009-08-12 网络出版日期:2009-06-11
  • 通讯作者: 王忠, E-mail: wangzhong@yzu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目（30871467）和江苏省高校研究生科技创新计划项目资助。

Relationship between cpt1 Gene and the Negative Phototropism in Rice Roots

WANG Yue-Xia12,WANG Zhong2*,LIU Quan-Jun1,ZHAO Hui-Jie1,GU Yun-Jie2,YUAN Zhi-Liang1   

  1. 1Coolege of Life Sciences，Henan Agricultural University，Zhengzhou 450002，China；2College of Biosciences and Biotechnology，Yangzhou University Yangzhou 225009，China
  • Received:2008-11-15 Revised:2009-02-17 Published:2009-08-12 Published online:2009-06-11
  • Contact: WANG Zhong, E-mail: wangzhong@yzu.edu.cn

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1838

被引次数

8

摘要：

为了研究cpt1基因与水稻根负向光性运动的关系，探讨IAA横向运输与水稻根负向光性运动的关系。检测了外源试剂CaCl2、EDTA以及IAA对水稻根的负向光性运动的效应，并采用RT-PCR的方法验证了不同外源试剂对cpt1基因表达的影响。结果表明，水稻根经光照24 h后，与对照相比，1 mg L-1的CaCl2和0.001 mg L-1的IAA能明显促进水稻根负向光性的弯曲生长，1 mg L-1的EDTA(CaCl2的抑制剂)明显抑制水根负向光性的弯曲生长；cpt1基因的表达受上述外源试剂诱导，并表现与水稻根的负向光性生长呈明显的正相关性，推测IAA的横向运输及其运输载体CPT1蛋白对水稻根的负向光性运动具重要作用。

关键词: 水稻, 负向光性, IAA载体蛋白, cpt1基因

Abstract:

With the purpose of studying the relationship between cpt1 gene and negative phototropism in rice roots, discussing the contribution of asymmetric distribution of IAA on negative phototropism in rice roots, CaCl2, EDTA and IAA were assayed for their effects on the negative phototropism in rice roots, as well as effects on the expression of cpt1 gene on the basis of reverse transcription-PCR. The result showed that, the negative phototropism in rice roots was improved by the treatment with 1 mg L-1 CaCl2 and 0.001 mg L-1 IAA in culture solution under light for 24 h but constrained by 1 mg L-1 EDTA. A similar effect was shown from the analysis of cpt1 gene expression, which suggested CPT1 protein could be induced by CaCl2 and IAA. The effects also showed a positive correlation between the expression of cpt1 and negative phototropism in rice roots. It could be supposed from present results that the asymmetric distribution of IAA is an important step for the negative phototropism process in rice roots, which probably is particularly associated with CPT1 protein as a carrier of IAA.

Key words: Rice, Root, Negative phototropism, IAA carrier protein, cpt1 gene

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