小麦白粉病菌诱导的TaWRKY34基因的鉴定与分析

doi:10.3724/SP.J.1006.2010.00249

摘要/Abstract

摘要：

WRKY转录因子在植物抗病防卫反应中发挥重要作用。利用cDNA宏阵列(macroarray)和RT-PCR相结合的方法，从小麦全长cDNA文库的WRKY转录因子中筛选出一个应答小麦白粉病菌胁迫的TaWRKY34转录因子，该基因编码464个氨基酸。染色体定位分析表明，该转录因子位于小麦第一同源群染色体的短臂上, 并且只在细胞核中表达。其蛋白序列与拟南芥、大麦和葡萄抗病相关WRKY转录因子的亲缘关系较近，与其中的3个WRKY基因具有相似的表达模式。TaWRKY34在Pm16/北京837⁷抗白粉病近等基因系中，对小麦白粉病菌、水杨酸和茉莉酸诱导的表达模式存在差异。TaWRKY34可能与小麦对白粉菌的抗性有关。

关键词: 小麦, 白粉病菌, WRKY转录因子, 染色体定位, 表达模式

Abstract:

WRKY transcription factors play important roles in plant defense signaling network. However, little is known about the biological roles of WRKY proteins in wheat(Triticum aestivum L.). The objectives of this study were to screen WRKY transcription factor genes conferring resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and disclose their function in wheat defense reaction. A WRKY transcription factor gene, TaWRKY34, was identified in response to Bgt by cDNA macroarray and semiquantitative RT-PCR from the wheat full-length cDNA libraries that were constructed in the authors’ earlier studies. This gene encodes 464 amino acid residues. TaWRKY34 was mapped onto short arms of chromosome 1B and 1D through blast search GrainGenes database and homemade full length cDNA library database of Aegilops tauschii. Further experiment indicated that TaWRKY34 also exists on chromosome 1AS through amplifying in Langdon D-genome disomic substitution lines and ChineseSpring nulli-tetrasomic lines with gene specific primers. Examining the subcellular localization of TaWARKY34, its coding region was fused to the 3’ end of green fluorescent protein (GFP). The GFP signal was detected only in the nucleus of onion epidermal cells to transiently express TaWRKY34-GFP, and the control-GFP protein distributed ubiquitously in both nuclei and cytoplasm. This suggests that TaWRKY34 is a nucleus-localized protein. Multiple sequence alignments of 57 WRKY domains from various species indicated that TaWRKY34 is closely related to WRKY transcription factors in response to pathogens in Arabidopsis thaliana (AtWRKY3, AtWRKY4 and AtWRKY33), Hordeum vulgare (HvWRKY42 and HvWRKY46), and Vitis vinifera (VvWRKY2) with identities ranging from 81.8% to 94.5%. Furthermore, TaWRKY34 has similar expression pattern with three sequences from A. thaliana, which was up-regulated at first and then down-regulated when inoculated with pathogens. The expression profiles of TaWRKY34 induced by powdery mildew fungus, salicylic acid and jasmonic acid were different between Pm16/Beijing 837⁷ near-isogenic lines (resistant to Bgt) and Beijing 837 (susceptible to Bgt). The results imply that TaWRKY34 is probably related to the resistance to powdery mildew in wheat.

Key words: Wheat, Blumeria graminis f. sp.tritici, WRKY transcription factor, Chromosome location, Expression pattern

秦伟,赵光耀,曲志才,张立超,段佳磊,李爱丽,贾继增,孔秀英. 小麦白粉病菌诱导的TaWRKY34基因的鉴定与分析[J]. 作物学报, 2010, 36(2): 249-255.

QIN Wei,ZHAO Guang-Yao,QU Zhi-Cai,ZHANG Li-Chao,DUAN Jia-Lei, LI Ai-LI,.

Identification and Analysis of TaWRKY34 Gene Induced by Wheat Powdery Mildew (Blumeria graminis f. sp. tritici)

[J]. Acta Agron Sin, 2010, 36(2): 249-255.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2010.00249

https://zwxb.chinacrops.org/CN/Y2010/V36/I2/249

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