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作物学报 ›› 2011, Vol. 37 ›› Issue (03): 469-476.doi: 10.3724/SP.J.1006.2011.00469

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于单片段代换系的水稻粒型QTL加性及上位性效应分析

赵芳明1,张桂权2,曾瑞珍2,杨正林1,凌英华1,桑贤春1,何光华1,*   

  1. 1 西南大学水稻研究所 / 农业部生物技术与作物品质改良重点开放实验室, 重庆 400716; 2 华南农业大学 / 广东省植物分子育种重点实验室, 广东广州 510642
  • 收稿日期:2010-06-19 修回日期:2010-12-01 出版日期:2011-03-12 网络出版日期:2011-01-17
  • 通讯作者: 何光华, E-mail: hegh@swu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金重点项目(30330370)，重庆市自然科学基金项目(CSTC,2006BB1330)，西南大学博士点基金项目和西南大学博士后基金项目资助。

Analysis of Epistatic and Additive Effects of QTLs for Grain Shape Using Single Segment Substitution Lines in Rice (Oryza sativa L.)

ZHAO Fang-Ming1,ZHANG Gui-Quan2,ZENG Rui-Zhen2,YANG Zheng-Lin1,LING Ying-Hua1,SANG Xian-Chun1,HE Guang-Hua1,*   

  1. 1 Rice Research Institute, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing 400716, China; 2 Guangdong Key Laboratory of Plant Molecular Breeding / South China Agricultural University, Guangzhou 510642,China
  • Received:2010-06-19 Revised:2010-12-01 Published:2011-03-12 Published online:2011-01-17
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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被引次数

34

摘要： 以分子标记辅助选择的手段有目的地进行基因聚合育种，对于加快育种进程具有重要的意义。而基因的加性和上位性效应是决定基因聚合能否成功的关键。本文以16个单片段代换系(SSSL)及15个双片段代换系分析了水稻粒型性状QTL的加性及上位性效应。共检测到9个水稻粒型性状QTL，包括4个粒长QTL、1个粒宽QTL和4个籽粒长宽比QTL，分别位于第2、第3、第4、第7和第10染色体上。此外，还检测出7对双基因互作，其中3对为有显著效应的两座位间互作，1对为两座位均没有显著效应的座位间互作，3对为1个有显著效应的座位与1个没有显著效应的座位间互作。本文结果进一步揭示了同一粒长QTL与不同单片段代换系聚合时会产生不同的互作效应，只有当上位性效应与目标基因的加性效应同向时，才可以达到明显改良粒长的效果。而且，2个长粒或2个短粒QTL聚合很难再产生更长或更短的籽粒。以上结果对于通过分子标记辅助育种手段改良水稻粒型具有重要意义。

关键词: 水稻, 单片段代换系, 粒型QTL, 加性效应, 上位性效应

Abstract: It is important to accelerate breeding process by pyramiding genes purposefully with molecular marker-assisted selection methods. Additive and epistatic effects of genes are the key to the success in genes pyramiding breeding. In this paper, additive and epistatic effects of QTLs for rice grain shape were analyzed using 16 single segment substitution lines (SSSL) and 15 double segment substitution lines (DSSL). A total of nine QTLs were identified on the Chromosomes 2, 3, 4, and 10 respectively, containing four for grain length, one for grain width and four for ratio of grain length to width. Furthermore, seven pairs of digenic interactions were detected for the grain shape. Among them, three interactions occurred between two loci with obvious effects on the traits, one interaction did not have obvious effects at both two loci, and three interactions occurred between one locus with and another without main effect at the single-locus level. Again, the results also indicated that the epistatic effects were different when the same QTL controlling grain length was pyramided with various SSSLs. Only when the epistatic effects were syntropic with the additive effects of the target genes, the pyramiding effect could be obvious. Moreover, longer or shorter grain was difficult to be obtained by pyramiding two QTLs controlling long grain or two QTLs for short grain. The results are important for improving grain shape by molecular marker assisted selection.

Key words: Rice, Single segment substitution lines, Grain shape QTL, Additive effects, Epistatic effects

[1]Luo Y-K(罗玉坤), Zhu Z-W(朱智伟), Chen N(陈能), Duan B-W(段彬伍), Zhang L-P(章林平). Grain types and related quality characteristics of rice in China. Chinese J Rice Sci (中国水稻科学), 2004, 18(2): 135-139 (in Chinese with English abstract)
[2]Xu Z-J(徐正进), Chen W-F(陈温福), Ma D-R(马殿荣), Lü Y-N(吕英娜), Zhou S-Q(周淑清), Liu L-X(刘丽霞). Correlations between rice grain shapes and main qualitative characteristics. Acta Agron Sin (作物学报), 2004, 30 (9): 894-900 (in Chinese with English abstract)
[3]Xing Y-Z(邢永忠), Tan Y-F(谈移芳), Xu C-G(徐才国), Hua J-P(华金平), Sun X-L(孙新立). Mapping quantitative trait loci for grain appearance traits of rice using a recombinant inbred line population. Acta Bot Sin (植物学报), 2001, 43(8): 840-845 (in Chinese with English abstract)
[4]Lei D-Y(雷东阳), Xie F-M(谢放鸣), Chen L-Y(陈立云). QTL mapping for grain appearance quality traits of advanced backcrossing introgression lines in rice. J Hunan Agricu Univ (Nat Sci)(湖南农业大学学报·自然科学版), 2009, 35(1): 1-4 (in Chinese with English abstract)
[5]Yan C-J(严长杰), Liang G-H(梁国华), Chen F(陈峰), Li Xin(李欣), Tang S-Z(汤述翥), Yi C-D(裔传灯), Tian S(田舜), Lu J-F(陆驹飞), Gu M-H(顾铭洪). Mapping quantitative trait loci associated with rice grain shape based on an indica/japonica backcross population. Acta Genet Sin (遗传学报), 2003, 30(8): 711-716 (in Chinese with English abstract)
[6]Zhang G-H(张光恒), Zhang G-P(张国平), Qian Q(钱前), Xu J-P(徐律平), Zeng D-L(曾大力), Teng S(滕胜), Bao J-S(包劲松). QTL analysis of grain shape traits in different environments. Chin J Rice Sci (中国水稻科学), 2004, 18(1): 16-22 (in Chinese with English abstract)
[7]Wang B(汪斌), Lan T(兰涛), Wu W-R(吴为人). Identification of QTL underlying grain traits in rice using SSLP linkage map. Fujian J Agric Sci (福建农业学报), 2003, 18(1): 11-15 (in Chinese with English abstract)
[8]Li Z-F(李泽福), Wan J-M(万建民), Xia J-F(夏加发), Zhai H-Q(翟虎渠). Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.). Acta Genet Sin (遗传学报), 2003, 30(3): 251-259 (in Chinese with English abstract)
[9]Tan Y-P(谭耀鹏), Li L-Z(李兰芝), Li P(李平), Wang L-X(王玲霞), Hu Z-L(胡中立). Quantitative trait loci for grain appearance traits of rice using a doubled haploid population. Mol Plant Breed (分子植物育种), 2005, 3(3): 314-322 (in Chinese with English abstract)
[10]Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics, 1995, 141: 1147-1162
[11]Xu H-S(徐华山), Sun Y-J(孙永建), Zhou H-J(周红菊), Yu S-B(余四斌). Development and characterization of contiguous segment substitution lines with background of an elite restorer line. Acta Agron Sin (作物学报), 2007, 33 (6): 979-986 (in Chinese with English abstract)
[12]Ebitani T, Takeuchi Y, Nonoue Y, Yamamoto T, Takeuchi K, Yano M. Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’ in a genetic background of japo- nica elite cultivar ‘Koshihikari’. Breed Sci, 2005, 55: 65-73
[13]Wan J-L(万建林), Zhai H-Q(翟虎渠), Wan J-M(万建民), Yasui H(安井秀), Yoshimura A(吉村淳). Mapping QTL for traits associated with resistance to ferrous iron toxicity in rice (Oryza sativa L.) using japonica chromosome segment substitution lines. Acta Genet Sin (遗传学报), 2003, 30(10): 893-898 (in Chinese with English abstract)
[14]Zhang G Q, Zeng R Z, Zhang Z M, Ding X H, Li W T, Liu G M, He F H, Tulukdar A, Huang C F, Xi Z Y, Qin L J, Shi J Q, ZhaoF M, Feng M J, Shan Z L, Chen L, Guo X Q, Zhu H T, Lu Y G. The construction of a library of single segment substitution lines in rice (Oryza sativa L.). Rice Genet Newsl, 2004, 21: 85-87
[15]Xi Z Y, He F H, Zeng R Z, Zhang Z M, Ding X H, Li W T, Zhang G Q. Development of a wide population of chromosome single segment substitution lines (SSSL) in the genetic background of an elite cultivar in rice (Oryza sativa L.). Genome, 2006, 49: 476-484
[16]Zeng R-Z(曾瑞珍), Shi J-Q(施军琼), Huang C-F(黄朝锋), Zhang Z-M(张泽民), Ding X-H(丁效华), Li W-T(李文涛), Zhang G-Q(张桂权). Development of a series of single segment substitution lines in indica background of rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2006, 32(1): 88-95 (in Chinese with English abstract)
[17]He F-H(何风华), Xi Z-Y(席章营), Zeng R-Z(曾瑞珍), Zhang G-Q(张桂权). Developing single segment substitution lines (SSSL) in rice (Oryza sativa L.) using advanced backcrosses and MAS. Acta Genet Sin (遗传学报), 2005, 32(8): 825-831 (in Chinese with English abstract)
[18]Gur A, Zamir D. Unused natural variation can lift yield barriers in plant breeding. PLoS Biol, 2004, 2: 1610-1615
[19]Zhao F M, Zhu H T, Ding X H, Zeng R Z, Zhang Z M, Li W T, Zhang G Q. Detection of QTLs for important agronomic traits and analysis of their stabilities using SSSLs in rice. Agric Sci China, 2007, 6(7): 769-778
[20]Zhao F M, Liu G F, Zhu H T, Ding X H, Zeng R Z, Zhang Z M, Li W T, Zhang G Q. Unconditional and conditional QTL mapping for tiller numbers at various stages with single segment substitution lines in rice (Oryza sativa L.). Agric Sci China, 2008, 7(3): 257-265
[21]Zhao F M, Zhang G Q, Zeng R Z, Yang Z L, Zhu H T, Zhong B Q, Ling Y H, He G H. Additive effects and epistasis effects of QTL for plant height and its components using single segment substitution lines (SSSL) in rice. Acta Agron Sin (作物学报), 2009, 35(1): 48-56
[22]Liao C Y, Wu P, Hu B, Yi K K. Effects of genetic background and environment on QTL and epistasis for rice (Oryza sativa L.) panicle number. Theor Appl Genet, 2001, 103: 104-111
[23]Jiang L-R(江良荣), Wang W(王伟), Huang J-X(黄建勋), Huang R-Y(黄荣裕), Zheng J-S(郑景生), Huang Y-M(黄育民), Wang H-C(王侯聪). Analysis of epistatic and QE interaction effects of QTL for grain shape in rice. Mol Plant Breed (分子植物育种), 2009, 7(4): 690-698 (in Chinese with English abstract)
[24]Eshed Y, Zamir D. Less-than-additive epistatic interactions of quantitative trait loci in tomato. Genetics, 1996, 143: 1807-1817
[25]Paterson A H, Deverna J W, Lanini B, Tanksley S D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato. Genetics, 1990, 124: 735-742
[26]Zeng R-Z(曾瑞珍), Tulukdar A, Liu F(刘芳), Zhang G-Q(张桂权). Mapping of the QTL controlling grain shape in rice using single segment substitution lines. Sci Agric Sin (中国农业科学), 2006, 39(4): 647-654 (in Chinese with English abstract)
[27]Wan X Y, Wan J M, Jiang L, Wang J K, Zhai H Q, Weng J F, Wang H L, Lei C L, Wang J L, Zhang X, Cheng Z J, Guo X P. QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects. Theor Appl Genet, 2006, 112: 1258-1270
[28]Fan C C, Xing Y Z, Mao H L, Lu T T, Han B, Xu C G, Li X H, Zhang Q F. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet, 2006, 112: 1164-1171
[29]Guo Y-M(郭咏梅), Mu P(穆平), Liu J-F(刘家富), Li Z-C(李自超), Lu Y-X(卢义宣). Correlation analysis and QTL mapping of grain shape and grain weight in rice under upland and lowland environments. Acta Agron Sin (作物学报), 2007, 33(1): 50-56 (in Chinese with English abstract)
[30]Chen B-X(陈冰嬬), Shi Y-Y(石英尧), Cui J-T(崔金腾), Qian Y-L(钱益亮), Liu H-Y(刘海燕), Zhang L-K(张力科), Wang H(王辉), Gao Y-M(高用明), Zhu L-H(朱苓华), Li Z-K(黎志康). QTL detection of grain size and shape with BC2F2 advanced backcross population of rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2008, 34(8): 1299-1307 (in Chinese with English abstract)
[31]Zhu W-Y(朱文银), Wang C-L(王才林), Yang L-Q(杨连群). Analysis of substituted segments in five advanced backcross substitution lines in an indica background in rice (Oryza sativa). Chin Bull Bot (植物学报), 2009, 44(6): 666-672 (in Chinese with English abstract)
[32]Rabiei B, Valizadeh M, Ghareyazie B, Moghaddam M, Ali A J. Identification of QTLs for rice grain size and shape of iranian cultivars using SSR markers. Euphytica, 2004, 137: 325-332
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