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作物学报 ›› 2012, Vol. 38 ›› Issue (12): 2198-2205.
蔡健1，张桂权2,*
CAI Jian1，ZHANG Qui-Quan2,*
摘要：
由华南农业大学选育的水稻单片段代换系S15对于野败型(WA)和矮败型(DA)细胞质雄性不育系均具有较强的恢复性。以野败型不育系博白A和矮败型不育系协青早A为母本, 单片段代换系S15为父本杂交, 采用分子标记辅助选择和连续回交的方法构建了两个BC3F2群体。利用与第1、第10染色体上恢复基因Rf3和Rf4两侧紧密连锁的SSR标记, 从这2个BC3F2群体中筛选携带基因型Rf3Rf3/rf4rf4和rf3rf3/Rf4Rf4的单株, 观察这些单株花粉和小穗育性, 并利用202个多态性SSR标记分析这些单株的遗传背景, 结果表明: (1)在同一细胞核背景下(S15), DA型细胞质的可恢复性好于WA型细胞质, 单片段代换系S15中的恢复基因Rf4的恢复力大于恢复基因Rf3的恢复力。(2)单片段代换系S15中的恢复基因对于WA型不育系博白A和DA型不育系协青早A表现出质量-数量性状的遗传。在单片段代换系S15中, 除了主效恢复基因Rf3和Rf4外, 微效基因或者修饰基因也表现出对于博白A和协青早A的恢复性作用, 而且效应较大。(3)在构建的2个BC3F2群体中, 携带基因型Rf3Rf3/rf4rf4和rf3rf3/Rf4Rf4单株的遗传背景片段数平均为1.0, 对应于恢复基因Rf3和Rf4座位的代换片段平均长度分别为12.9 cM和18.4 cM。
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