欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (03): 409-422.doi: 10.3724/SP.J.1006.2013.00409

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

三个中籼稻骨干恢复系对白叶枯病和褐飞虱抗性改良效果的评价

陈凯1,2,张强1,2,潘晓飚3,李美4,孟丽君4,徐正进1,*,徐建龙2,*,黎志康2   

  1. 1 沈阳农业大学 / 农业部作物生理生态遗传育种重点开放实验室, 辽宁沈阳110161; 2 中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程, 北京100081; 3 台州市农业科学研究所, 浙江临海317000; 4 中国农业科学院深圳生物育种创新研究院, 广东深圳 518083
  • 收稿日期:2012-10-22 修回日期:2012-11-16 出版日期:2013-03-12 网络出版日期:2013-01-04
  • 通讯作者: 徐建龙, E-mail: xujlcaas@yahoo.com.cn, Tel: 010-82105854; 徐正进, E-mail: xuzhengjin@126.com
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目(2011-G2B)和国家高技术研究发展计划(863计划)项目(2012AA101101)资助。

Evaluation on Effect of Resistance Improvement of Bacterial Leaf Blight and Brown Planthopper for Three Dominant Indica Restorer Lines

CHEN Kai1,2,ZHANG Qiang1,2,PAN Xiao-Biao3,LI Mei4,MENG Li-Jun4,XU Zheng-Jin1,*,XU Jiang-Long2,*,LI Zhi-Kang2   

  1. 1 Shenyang Agricultural University/Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang 110161, China;
    2 Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Taizhou Institute of Agricultural Sciences, Linhai 317000, China; 4 Shenzhen Institute of Biological Breeding & Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518083, China
  • Received:2012-10-22 Revised:2012-11-16 Published:2013-03-12 Published online:2013-01-04

摘要:

利用分子标记辅助选择和田间鉴定选择相结合的方法,将抗白叶枯病基因Xa23和抗褐飞虱基因Bph18(t)导入明恢86、蜀恢527和浙恢79543个骨干中籼恢复系,获得带有Xa23抗性基因纯合的改良恢复系明恢86-Xa23、蜀恢527-Xa23、浙恢7954-Xa23携有抗褐飞虱Bph18(t)基因的蜀恢527-Bph18(t)、浙恢7954-Bph18(t),并从蜀恢527/IRBB23 F1和浙恢7954/IR65482 F1复交后代中选育出的带有Xa23Bph18(t)的双基因聚合系浙蜀-Xa23-Bph18(t)。明恢86-Xa23、蜀恢527-Xa23、浙恢7954-Xa23浙蜀-Xa23-Bph18(t)对中国和菲律宾的17个白叶枯病菌均表现高抗,蜀恢527-Bph18(t)、浙恢7954-Bph18(t)和浙蜀-Xa23-Bph18(t)对褐飞虱的抗性也达到中抗以上水平。抗性改良恢复系及其与不育系II-32A、沪旱11A的测交种在不接种白叶枯病菌条件下的产量和结实率与原来的恢复系及相应杂交种相仿,但在接种条件下带有Xa23基因的恢复系及测交种的结实率和产量明显优于原来的恢复系及相应杂交种。研究表明,抗性基因Xa23在不同恢复系背景下的抗性表达完全,对恢复系白叶枯病改良的效果明显,而抗性基因Bph18(t)对褐飞虱的改良效果与遗传背景有关。对分子标记回交和复交改良恢复系的抗病虫性进行了讨论。

关键词: 水稻恢复系, 白叶枯病, 褐飞虱, 抗性改良, 标记辅助选择

Abstract:

A novel bacterial blight (BB) resistance gene, Xa23, and brown planthopper (BPH) resistance gene, Bph18(t) were introgressed into three popular restorer lines (Minghui 86, Shuhui 527, and Zhehui 7954) for wild abortive cytoplasmic male sterility by marker-assisted backcross and pyramiding breeding approaches in combination with artificial inoculation and stringent phenotypic selections. The four derived BB resistant restorer lines [Minghui 86-Xa23, Shuhui 527-Xa23, Zhehui 7954-Xa23,and Zheshu-Xa23-Bph18(t)] and their derived hybrid combinations with II-32A and Huhan11A demonstrated similar BB resistance spectrum as the donor parent, IRBB23. The three derived BPH resistant restorer lines [Shu 527-Bph18(t), Zhehui 7954-Bph18(t), and Zheshu-Xa23-Bph18(t)] showed resistant or moderate resistant to BPH. The newly developed BB and BPH resistant restorers and their derived hybrids were identical to their respective original versions for agronomic traits especially under disease free condition. However, under severe disease condition, the four BB resistant restorer lines exhibited significantly higher grain weight and spikelet fertility as compared to the respective original restorer lines thus further resulting in BB resistant hybrids with significantly higher grain yields than their respective original hybrids. The results indicated that the Xa23 gene could completely express its dominant resistance in different backgrounds of both restorer and male sterile lines across different growth stages, suggesting its immense breeding value in BB resistance improvement for hybrid rice. However, effect of resistant improvement of Bph18(t) for BPH depends on genetic background. Improvement of resistance of BB and BPH for restorer lines by molecular marker-assisted backcross and pyramiding was deeply discussed.

Key words: Rice restorer line, Bacterial leaf blight, Brown planthopper, Resistance improvement, Marker-assisted selection

[1]Ou S H. Rice Diseases, 2nd edn. Kew, England: Common wealth Mycological Institute, 1985



[2]Singh G P, Srivastava M K, Singh R V. Variation and qualitative losses caused by bacterial blight in difference rice varieties. Indian Phytopath, 1997, 30: 180–185



[3]Zhai W-X(翟文学), Zhu L-H(朱立煌). Rice bacterial blight resistance genes and their utilization in molecular breeding. Prog Biotechnol (生物工程进展), 1999, 19(6): 9–15 (in Chinese with English abstract)



[4]Zhang J-F(张珏锋), Wu H(吴鸿), Chen J-M(陈建明), Zheng X-S(郑许松), Chen L-Z(陈列忠), Yu X-P(俞晓平). A strain isolated from brown planthopper and its molecular identification.Chin J Rice Sci (中国水稻科学), 2007, 21(5): 551–554 (in Chinese with English abstract)



[5]Xu M-R(许美荣), Zhou Y-L(周永力), Li Z-K(黎志康). Research advances in on resistance genetics of rice bacterial blight and bacterial leaf streak. J Plant Genet Resour (植物遗传资源学报), 2012, 13(3): 370–375 (in Chinese with English abstract)



[6]Zhang Q(章琦). Genetics and improvement of resistance to bacterial blightin hybrid rice in China. Chin J Rice Sci (中国水稻科学), 2009, 23(2): 111–119 (in Chinese with English abstract)



[7]Zhang Q(章琦), Wang C-L(王春连), Zhao K-J(赵开军), Yang W-C(杨文才), Qiao F(乔枫), Zhou Y-L(周永力), Jiang Q-X(江祺祥), Liu G-C(刘古春). Development of near-isogenic line CBB23 with a new resistance gene to bacterial blight in rice and its application. Chin J Rice Sci (中国水稻科学), 2002, 16(3): 206–210 (in Chinese with English abstract)



[8]Luo Y-C(罗彦长), Wu S(吴爽), Wang S-H(王守海), Li C-Q(李成荃), Zhang D-P(张端品), Zhang Q(章琦), Zhao K-J(赵开军), Wang C-L(王春连), Wang D-Z(王德正), Du S-Y(杜士云), Wang W-X(王文相). Pyramiding two bacterial blight resistance genes into a CMS line R106A in rice. Sci Agric Sin (中国农业科学), 2005, 38(11): 2157–2164 (in Chinese with English abstract)



[9]Luo Y-C (罗彦长), Wang S-H (王守海), Li C-Q(李成荃), Wu S (吴爽), Wang D-Z(王德正), Du S-Y(杜士云). Improvement of bacterial blight resistance by molecular marker-assisted aelection in a wide compatibility restorer line of hybrid rice. Chin J Rice Sci (中国水稻科学), 2005, 19(1): 36–40 (in Chinese with English abstract)



[10]Wang C-L(王春连), Qi H-X(戚华雄), Pan H-J(潘海军), Li J-B(李进波), Fan Y-L(樊颖伦), Zhang Q(章琦), Zhao K-J(赵开军). EST-markers flanking the rice bacterial blight resistance gene Xa23 and their application in marker-assisted selection. Sci Agric Sin (中国农业科学), 2005, 38(10): 1996–2001 (in Chinese with English abstract)



[11]Li J-B(李进波), Wang C-L(王春连), Xia M-Y(夏明元), Zhao K-J(赵开军), Qi H-X(戚华雄), Wan B-L(万丙良), Zha Z-P(查中萍), Lu X-G(卢兴桂). Enhancing bacterial blight resistance of hybrid rice restorer lines through marker-assisted selection of the Xa23 gene. Acta Agron Sin (作物学报), 2006, 32(10): 1423–1429 (in Chinese with English abstract)



[12]Zheng J-T(郑家团), Tu S-H(涂诗航), Zhang J-F(张建福), Zheng Y(郑轶), Zhao K-J(赵开军), Zhang S-J(张水金), Xie H-A(谢华安). Breeding of restorer lines of hybrid rice with bacterial blight resistance gene Xa23 by using marker-assisted selection. Chin J Rice Sci (中国水稻科学), 2009, 23(4): 437–439 (in Chinese with English abstract)



[13]Yang H-N(阳海宁), Wei S-L(韦绍丽), Li X-Q(李孝琼), Zhang Y-X(张月雄), Liu C(刘驰), Chen Q(陈乔), Cen Z-L(岑贞陆), Wei S-M(韦素美), Yang L(杨朗), Huang F-Q(黄凤宽), He W-Q(何文强), Liu K-Y(刘开雨), Huang D-H(黄大辉), Ma Z-F(马增凤), Huang Y-Y(黄悦悦), Song J-D(宋建东), Li R-B(李容柏). Rice pyramiding lines conferring resistances to brown planthopper and bacterial leaf blight by marker-assisted selection. Mol Plant Breed (分子植物育种), 2010, 8(1): 11–19 (in Chinese with English abstract)



[14]Chen J-M(陈建民), Fu Z-Y(付志英), Quan B-Q(权宝权), Tian D-G(田大刚), Li G(李刚), Wang F(王锋). Breeding hybrid rice restoring line with double resistance to rice blast and bacterial blight by marker assisted selection. Mol Plant Breed (分子植物育种), 2009, 7(3): 465–470 (in Chinese with English abstract)



[15]Xia Z-H(夏志辉), Zhao X-F(赵显峰), Fan H-K(范海阔), Jin L(金良), Gao L-F(高利芬), Luo Y-H(罗越华), Zhai W-X(翟文学). Improving of bacterial blight resistance of hybrid rice parents through marker-assisted-selection of the Xa23 gene. Mol Plant Breed (分子植物育种), 2010, 8(4): 652–656 (in Chinese with English abstract)



[16]Liang Y-T(梁云涛), Wang C-L(王春连), Liu P-Q(刘丕庆), Fu Q(傅强), Kshirod K J, Zhao K-J(赵开军). Research and application status of rice genes resistance to brown planthopper. J Plant Genet Resour (植物遗传资源学报), 2008, 9(1): 119–124 (in Chinese with English abstract)



[17]Rahman M L, Jiang W Z, Chu S H, Qiao Y L, Ham T H, Woo M O, Lee J Y, Khanam M S, Chin J H, Jeung J U. High resolution mapping of two rice brown plantbopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minula. Theor Appl Genet, 2009, 119: 1237–1246



[18]Su C-C(苏昌潮), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Wan J-M(万建民). Progress in studies on genetics of resistance to rice brown planthopper (Nilaparvata lugens Stal) and breeding of resistant cultivars. Hybrid Rice (杂交水稻), 2003, 18(4): 1–6 (in Chinese without English abstract)



[19]Jena K K, Jeung J U, Lee J H, Choi H C, Brar D S. High resolution mapping of a new brown plant bopper(BPH) resistance gene, Bph18(t), and marker assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet, 2006, 112: 288–297



[20]Liang Y-T(梁云涛), Wang C-L(王春连), Lai F-X(赖凤香), Liu P-Q(刘玉庆), Wang J(王坚), Fu Q(傅强), Zhao K-J(赵开军). Development of STS marker KC1 for brown planthopper resistance gene Bph18(t) and marker assisted selection efficiency. Chin J Rice Sci (中国水稻科学), 2010, 24(3): 244–250 (in Chinese with English abstract)



[21]Ni D-H(倪大虎), Yi C-X(易成新), Li L(李莉), Wang X-F(汪秀峰), Zhang Y(张毅), Zhao K-J(赵开军), Wang C-L(王春连), Zhang Q(章琦), Wang W-X(王文相), Yang J-B(杨剑波). Developing rice lines resistant to bacterial blight and blast with molecular marker-assisted selection. Acta Agron Sin (作物学报), 2008, 34(1): 100–105 (in Chinese with English abstract)



[22]Liu G-J(刘光杰), Fu Z-H(付志红), Shen J-H(沈君辉), Zhang Y-H(张亚辉). Comparative study on evaluation methods for resistance to rice planthoppers (Homoptera: Delphacidae) in rice. Chin J Rice Sci (中国水稻科学), 2002, 16(1): 52–56 (in Chinese with English abstract)



[23]Zheng X-P(郑秀平), Lin Q(林强), Wu Z-Y(吴志源), Zhou T-L(周天理). Study on technology and path of the super rice breeding. Chin Agric Sci Bull (中国农学通报), 2006, 22(7): 205–508 (in Chinese with English abstract)



[24]Zhang Q(章琦). High lights in identification and application of resistance genes to bacterial blight. Chin J Rice Sci (中国水稻科学), 2005, 19(5): 453–459 (in Chinese with English abstract)



[25]Pei Q-L(裴庆利), Wang C-L(王春连), Liu P-Q(刘丕庆), Wang J(王坚), Zhao K-J(赵开军). Marker-assisted selection for pyramiding disease and insect resistance genes in rice. Chin J Rice Sci (中国水稻科学), 2011, 25(2): 119–129 (in Chinese with English abstract)



[26]Zha R M, Ling Y H, Yang Z L. Prediction of hybrid grain yield performances in indica rice (Oryza sativa L.) with effect-increasing loci. Mol Breed, 2008, 22: 467–476



[27]Chen S, Lin X H, Xu C G, Zhang Q. Improvement bacterial blight resistance of ‘Minhgui 63’ an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Sci, 2000, 40: 239–244



[28]Joseph M, Gopalakrishnan S, Sharma R K, Singh V P, Singh A K, Singh N K, Mohapatra T. Combining bacterial blight resistance and Basmati quality characteristics by phenotypic and molecular marker-assisted selection in rice. Mol Breed, 2004, 13: 377–387



[29]Sundaram R M, Vishnupriya M R, Biradar S K, Laha G S, Reddy G A, Rani N S, Sarma N P, Sonti R V. Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica, 2008, 160: 411–422



[30]Zhai W X, Wang W M, Zhou Y L, Li X B, Zheng X W, Zhang Q, Wang G L, Zhu L H. Breeding bacterial blight-resistant hybrid rice with the cloned bacterial blight resistance gene Xa21. Mol Breed, 2001, 8: 285–293



[31]Cao L-Y(曹立勇), Zhuang J-Y(庄杰云), Zhan X-D(占小登), Zheng K-L(郑康乐), Cheng S-H(程式华). Hybrid rice resistant to bacterial blight developed by marker assisted selection. Chin J Rice Sci (中国水稻科学), 2003, 17(2): 184–186 (in Chinese with English abstract)



[32]Tong H-J(童海军), Xue Q-Z(薛庆中). II you 8220, a new disease resistant hybrid rice combination. Hybrid Rice (杂交水稻), 2003, 18(5): 73–74 (in Chinese with English abstract)



[33]Peng Y-C(彭应财), Li W-H(李文宏), Fang Y-P(方又平), Fan Y-Y(樊叶扬). Breeding and application of indica hybrid rice Zhongyou 218 by molecular marker-assisted selection. Hybrid Rice (杂交水稻), 19(3): 13–16 (in Chinese with English abstract)



[34]Cao L-Y(曹立勇), Zhan X-D(占小登), Zhuang J-Y(庄杰云), Cheng S-H(程式华). Breeding of indica hybrid rice Guodao 1 with good quality, high yield and resistance to bacterial leaf blight by marker-assisted selection technique. Hybrid Rice (杂交水稻), 2005, 20(3): 16–18 (in Chinese with English abstract)



[35]Huang N, Angeles E R, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush G S. Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet, 1997, 95: 313–320



[36]Sanchez A C, Brar D S, Huang N, Li Z, Khush G S. Sequence tagged site markerassisted selection for three bacterial blight resistance genes in rice. Crop Sci, 2000, 40: 792–797



[37]Singh S, Sidhu J S, Huang N, Vikal Y, Li Z, Brar D S, Dhaliwal H S, Khush G S. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet, 2001, 102: 1011–1015



[38]Yohsimuar S, Yoshimuar A, Iwata N, McCouch S R, Abenes M L, Baraoidna M R, Mew T W, Nelosn R J. Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Mol Breed, 1995, 1: 375–387



[39]Deng Q-M(邓其明), Wang S-Q(王世全), Zheng A-P(郑爱萍), Zhang H-Y(张红宇), Li P(李平). Breeding restorer lines with high resistance to bacterial blight in hybrid rice by using molecular marker-assisted selection. Chin J Rice Sci (中国水稻科学), 2006, 20(2): 153–158 (in Chinese with English abstract)
[1] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[2] 马红勃, 刘东涛, 冯国华, 王静, 朱雪成, 张会云, 刘静, 刘立伟, 易媛. 黄淮麦区Fhb1基因的育种应用[J]. 作物学报, 2022, 48(3): 747-758.
[3] 赵海涵, 练旺民, 占小登, 徐海明, 张迎信, 程式华, 楼向阳, 曹立勇, 洪永波. 水稻协优9308重组自交系群体白叶枯病抗性的全基因组关联分析[J]. 作物学报, 2022, 48(1): 121-137.
[4] 韩玉洲, 张勇, 杨阳, 顾正中, 吴科, 谢全, 孔忠新, 贾海燕, 马正强. 小麦株高QTL Qph.nau-5B的效应评价[J]. 作物学报, 2021, 47(6): 1188-1196.
[5] 黄义文, 代旭冉, 刘宏伟, 杨丽, 买春艳, 于立强, 于广军, 张宏军, 李洪杰, 周阳. 小麦多酚氧化酶基因Ppo-A1Ppo-D1位点等位变异与穗发芽抗性的关系[J]. 作物学报, 2021, 47(11): 2080-2090.
[6] 郑凯丽, 纪志远, 郝巍, 唐永超, 韦叶娜, 胡运高, 赵开军, 王春连. 水稻白叶枯病感病相关基因Xig1的分子鉴定及抗病资源创制[J]. 作物学报, 2020, 46(9): 1332-1339.
[7] 胡茂龙, 程丽, 郭月, 龙卫华, 高建芹, 浦惠明, 张洁夫, 陈松. 油菜抗咪唑啉酮类除草剂基因标记的开发与应用[J]. 作物学报, 2020, 46(10): 1639-1646.
[8] 张平,姜一梅,曹鹏辉,张福鳞,伍洪铭,蔡梦颖,刘世家,田云录,江玲,万建民. 通过分子标记辅助选择将耐储藏主效QTL qSS-9 Kas转入宁粳4号提高其种子贮藏能力[J]. 作物学报, 2019, 45(3): 335-343.
[9] 杨勇,陆彦,郭淑青,石仲慧,赵杰,范晓磊,李钱峰,刘巧泉,张昌泉. 籼稻背景下导入Wx in等位基因改良稻米食味和理化品质[J]. 作物学报, 2019, 45(11): 1628-1637.
[10] 张安宁,刘毅,王飞名,谢岳文,孔德艳,聂元元,张分云,毕俊国,余新桥,刘国兰,罗利军. 节水抗旱稻恢复系的抗褐飞虱分子标记辅助选育及抗性评价[J]. 作物学报, 2019, 45(11): 1764-1769.
[11] 李玉玲,蒋正宁,胡文静,李东升,程婧晔,裔新,程晓明,吴荣林,程顺和. CIMMYT小麦种质C615抗叶锈病QTL分析[J]. 作物学报, 2018, 44(6): 836-843.
[12] 田宇,杨蕾,李英慧,邱丽娟. 抗大豆胞囊线虫SCN3-11位点的KASP标记开发和利用[J]. 作物学报, 2018, 44(11): 1600-1611.
[13] 朱展望, 徐登安, 程顺和, 高春保, 夏先春, 郝元峰, 何中虎. 中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源[J]. 作物学报, 2018, 44(04): 473-482.
[14] 张宏军, 宿振起, 柏贵华, 张旭, 马鸿翔, 李腾, 邓云, 买春艳, 于立强, 刘宏伟, 杨丽, 李洪杰, 周阳. 利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性[J]. 作物学报, 2018, 44(04): 505-511.
[15] 姚姝,陈涛,张亚东,朱镇,赵庆勇,周丽慧,赵凌,赵春芳,王才林. 利用分子标记辅助选择聚合水稻Pi-taPi-bWx-mq基因[J]. 作物学报, 2017, 43(11): 1622-1631.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!