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作物学报 ›› 2014, Vol. 40 ›› Issue (07): 1311-1319.doi: 10.3724/SP.J.1006.2014.01311

• 研究简报 • 上一篇    下一篇

国内外蚕豆核心种质SSR遗传多样性对比及微核心种质构建

姜俊烨1,杨涛1,王芳1，方俐1，仲伟文2，关建平1，宗绪晓1,*   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京 100081; 2 甘肃农业大学生命科学技术学院, 甘肃兰州 730070
  • 收稿日期:2013-11-20 修回日期:2014-04-16 出版日期:2014-07-12 网络出版日期:2014-05-16
  • 通讯作者: 宗绪晓, E-mail: zongxuxiao@caas.cn, Tel: 010-62186651
  • 基金资助:

    本研究由科技部科技支撑项目(2013BAD01B05), 科技部国际科技合作项目(2010DFB33340), 国家自然科学基金项目(31101198)和农业部作物种质资源保护项目(NB2013-2129135-25)资助。

Genetic Diversity Analysis of Germplasm Resources and Construction of Mini-core Collections for Vicia faba L. at Home and Abroad

JIANG Jun-Ye1,YANG Tao1,WANG Fang1,FANG Li1,ZHONG Wei-Wen2,GUAN Jian-Ping1,ZONG Xu-Xiao1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China?
  • Received:2013-11-20 Revised:2014-04-16 Published:2014-07-12 Published online:2014-05-16
  • Contact: 宗绪晓, E-mail: zongxuxiao@caas.cn, Tel: 010-62186651

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被引次数

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摘要：

运用24对SSR引物, 对国内外1075份初级地理蚕豆核心种质的遗传多样性分析显示, 等位变异数、有效等位变异数及Shannon’s信息指数分别为8.54、2.26和1.02；对全部参试资源进行聚类分析, 没有发现明显的群体结构, 表明初级地理核心种质的代表性较好, 遗传背景较广泛。之后采用每个类内随机抽样的方法构建含有129份国内资源和63份国外资源的蚕豆微核心种质, 等位基因变异数、有效等位变异数和Shannon’s信息指数的保留比例分别为87.32%, 101.26%, 101.82%；经t检验得出微核心种质与全部参试资源群体间遗传多样性差异不显著, 表明构建的微核心种质的遗传多样性可以代表初级地理蚕豆核心种质。

关键词: SSR, 蚕豆, 遗传多样性, 核心种质, 微核心种质

Abstract:

The genetic diversity of 1075 genotypes from a primary geographic core collection of faba bean (Vicia faba L.) was analyzed by using SSR markers. The number of observed and effective alleles of 8.54 and 2.26, the Shannon’s information index of 1.02, were detected. Unconspicuous population structure among the 1075 genotypes indicated an extensive genetic background and nice representativeness. A mini-core collection comprised 129 Chinese and 63 oversea faba bean genotypes was randomly sampled from each cluster of dendrogram including all the 1075 tested genotypes. The observed alleles, effective alleles and Shannon’s information index of the mini-core collection covered 87.32%, 101.26%, and 101.82% that of primary geographic core collection respectively. The t-test did not show significant differences in genetic diversity between the primary geographic core collection and the mini-core collection, which validated the representativeness of the mini-core collection in genetic diversity of faba bean. The mini-core collection will play an important role in exploration of new genes within faba bean.

Key words: SSR, Faba bean, Genetic diversity, Geographic core collection, Mini-core collection

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