作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1540-1548.doi: 10.3724/SP.J.1006.2014.01540
王诺菡1,2,于霁雯2,吴嫚2,马启峰1,李兴丽2,裴文锋2,李海晶2,黄双领2,张金发2,喻树迅1,2,
WANG Nuo-Han1,2,YU Ji-Wen2,WU Man2,MA Qi-Feng1,LI Xing-Li2,PEI Wen-Feng2,LI Hai-Jing2,HUANG Shuang-Ling2,ZHANG Jin-Fa2,YU Shu-Xun1,2,
摘要:
MYB类转录因子是植物转录因子最大的家族之一, 参与控制植物腺毛细胞的模式和形态建成。本研究利用雷蒙德氏棉(Gossypium raimondii) D5基因组数据库以AtMYB0 (GL1, NM_113708)蛋白为参比序列获得同源基因GrMYB0, 从徐州142中克隆了陆地棉的GhMYB0, 其开放阅读框长度为843 bp, 编码280个氨基酸。经过保守结构域分析和亚细胞定位确定GhMYB0为R2R3-MYB转录因子。qRT-PCR的结果表明, GhMYB0在徐州142开花当天开始高调表达, 开花后20 d表达量达高峰; 在所有的组织器官中, 花中表达量最高, 其次为胚珠。转基因功能分析结果表明, 在野生型拟南芥(Columbia)中过表达GhMYB0, 使其叶片表皮毛与野生型相比明显减少; 该基因在拟南芥突变体gl-1中过表达, 能恢复表皮毛缺失型突变体的表型, 说明该基因可能对拟南芥表皮毛的形态建成发挥一定作用, 本试验为研究R2R3-MYB转录因子在棉纤维起始和伸长过程中的调控作用提供有力证据。
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