作物学报 ›› 2015, Vol. 41 ›› Issue (03): 507-514.
• 研究简报 • 上一篇
张桂芳1,2,丁在松1,赵明1,*
ZHANG Gui-Fang1,2,DING Zai-Song1,ZHAO Ming1,*
摘要:
稗草(Echinochloa crusgalli)是稻田中的C4 光合型杂草, 为了探索稗草ppc 基因(Eppc)对水稻遗传转化的可行性及其对光合速率的调节效应, 首次将含有稗草根型磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase, PEPC)基因ppc cDNA 的2 个植物表达载体pUbi-Eppc、pRbcS-Eppc 通过农杆菌介导法对水稻进行了遗传转化。对分化植株进行的PCR、RT-PCR、克隆测序和Western 杂交等结果均表明稗草ppc 基因已经整合到了水稻基因组中, 并且在转录和翻译水平都得到了表达。转基因水稻PEPC 活性和气体交换参数测定结果表明T0 代多数植株的PEPC 活性高于对照, 最高达到了对照的5.85 倍; T0 代大多数转基因植株叶片的净光合速率(Pn)比对照提高了20.00%, 最大地提高了47.16%, 同时叶片水分利用效率(WUE)也得到增强; T6 代大部分转化植株的PEPC 活性及Pn 仍保持高于对照, 本研究表明C3 根型ppc 基因过量表达也可以提高水稻的Pn, 且证明稗草PEPC 对光合作用具有较强的调节作用。
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