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作物学报 ›› 2016, Vol. 42 ›› Issue (08): 1168-1175.doi: 10.3724/SP.J.1006.2016.01168

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

宁麦9号及其衍生品种(系)揉混特性的关联分析

姜朋,张平平,张旭,陈小霖,马鸿翔*   

  1. 江苏省农业科学院/江苏省农业生物学重点实验室/江苏省现代作物生产协同创新中心,江苏南京 210014
  • 收稿日期:2015-11-17 修回日期:2016-03-14 出版日期:2016-08-12 网络出版日期:2016-05-09
  • 通讯作者: 马鸿翔, E-mail: hxma@jaas.ac.cn
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-3), 江苏省农业自主创新资金项目(CX14-2002)和江苏省科技支撑计划项目(BE2013439, BE2015352)资助。

Association Analysis for MixographProperties in Ningmai 9 and Its Derivatives

JIANG Peng,ZHANG Ping-Ping,ZHANG Xu,CHEN Xiao-Lin,MA Hong-Xiang*   

  1. Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Key Laboratory for Agrobiology / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, China
  • Received:2015-11-17 Revised:2016-03-14 Published:2016-08-12 Published online:2016-05-09
  • Contact: 马鸿翔, E-mail: hxma@jaas.ac.cn
  • Supported by:

    Thisstudy was supported by China Agriculture Research System (CARS-3), Jiangsu Provincial Fundfor Independent Innovation in Agricultural Sciences(CX14-2002),andJiangsu Science & Technology Pillar Program(BE2013439, BE2015352).

摘要:

揉混特性是评价面团流变学特性的重要指标,影响面食产品的品质。宁麦9号曾是我国长江中下游小麦生产中大面积应用的优质软质小麦品种,而且以其为亲本育成了17个小麦品种。研究与宁麦9号揉混特性相关的分子标记可为该品种在软质小麦品种育种中的应用提供依据。以宁麦9号及其117个衍生品种(系)为材料,利用覆盖小麦全基因组的185对多态性SSR引物对其进行基因组扫描,并结合2009–2010和2010–2011生长季的揉混参数表型数据,应用混合线性模型(Mixed-linear Model, MLM)进行全基因组关联分析,发掘与其相关联的分子标记位点。共检测到13个与揉混参数相关联的标记位点(P<0.01),表型贡献率为5.71%~12.33%。其中,与和面时间关联的有3个,Xwmc594连续两年被检测到;与峰值高度关联的有3个;与峰值宽度关联的有2个,Xgwm299连续两年被检测到;与8 min带宽关联的7个中,4个(Xwmc11、Xbarc320、Xbarc110和Xgwm577)连续两年被检测到。此外,Xwmc594和Xgwm577同时与和面时间、8 min带宽相关联。以上6个稳定的关联位点均对相应揉混参数起负向调控作用,其连锁的分子标记有望应用于以宁麦9号为亲本的软质小麦遗传改良。

关键词: 小麦, 揉混参数, 关联分析, 分子标记

Abstract:

The mixograph property is one of the rheological properties affecting the quality of end-use products in wheat. Ningmai 9 isa soft wheat cultivar planted largely in the middle and lower reaches of Yangtze River and served as a main parent of 17released wheat cultivars. For the purpose of utilizing Ningmai 9 in molecular marker-assisted selection, 185 polymorphic SSRmarkers associated with mixograph properties were tested in Ningmai 9 and its 117 derivatives. The mixed-linear model (MLM) was used in combination of phenotypic data collected in the 2009–2010 and 2010–2011 growing seasons. A total of 13 SSRs were identified to be significantly associated with mixograph properties (P<0.01), explaining 5.71–12.33% of phenotypic variations.Among the 13 markers, three, three, two, and seven were associated with mix time (MT),peak height (PH), peak width (PW), eight-minute width (8MW),respectively. MarkersXwmc11, Xbarc320, Xbarc110,and Xgwm577 for 8MW,Xwmc594 for MT,and Xgwm299 for PWwere identified over two years. Besides, Xwmc594 and Xgwm577 were associated with both (MT) and 8MW. The six markers identified over two years all negativelyaffectedmixograph parametersat Ningmai 9 background and might be used in softwheat breeding.

Key words: Wheat, Mixograph parameters, Association analysis, Molecular markers

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