利用水稻MAGIC群体关联定位白叶枯病抗性QTL和创制抗病新种质

作物学报 ›› 2016, Vol. 42 ›› Issue (10): 1437-1447.

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

利用水稻MAGIC群体关联定位白叶枯病抗性QTL和创制抗病新种质

陈天晓^1,2,朱亚军³,密雪飞³,陈凯³,孟丽君³,左示敏^1,*,徐建龙^2,3,4,*

1扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心,教育部植物功能基因组学重点实验室，江苏扬州225009；2中国农业科学院作物科学研究所，北京100081；3中国农业科学院深圳农业基因组研究所，广东深圳518210；4中国农业科学院深圳生物育种创新研究院，广东深圳518210

收稿日期:2016-03-07 修回日期:2016-06-20 出版日期:2016-10-12 网络出版日期:2016-07-04
通讯作者: 徐建龙,E-mail:xujlcaas@126.com,Tel:010-82105854;左示敏,E-mail:smzuo@yzu.edu.cn,Tel:0514-87972136
基金资助:
本研究由国家高技术研究发展计划(863计划)项目(2014AA10A601)，深圳孔雀团队计划项目(20130415095710361)，中国农业科学院科技创新工程团队和江苏重点研发计划(现代农业)(BE2015363)项目资助。

Mapping of QTLs for Bacterial Blight Resistance and Screening of Resistant Materials Using MAGICP opulations of Rice

CHEN Tian-Xiao1,2, ZHU Ya-Jun3, MI Xue-Fei3, CHEN Kai3, MENG Li-Jun3, ZUO Shi-Min1,*, and XU Jian-Long2,3,4,*

1 Key Laboratory of Plant Functional Genomics of Jiangsu Province / Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518210, China; 4 Shenzhen Institute of Breeding & Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China

Received:2016-03-07 Revised:2016-06-20 Published:2016-10-12 Published online:2016-07-04
Contact: 徐建龙,E-mail:xujlcaas@126.com,Tel:010-82105854;左示敏,E-mail:smzuo@yzu.edu.cn,Tel:0514-87972136

摘要/Abstract

摘要：

以8个不同亲本构建的遗传上相互关联的多亲本高代互交系(multi-parents advanced generation inter-cross, MAGIC)群体，包括2个4亲本群体(DC1和DC2)和1个8亲本群体(DC3)为材料，接种我国白叶枯病强致病力V型菌系(GD-V)和弱致病力II型菌系(C2)，关联分析定位MAGIC群体对白叶枯病的抗性QTL，筛选抗病种质。结果表明，大多数亲本对C2菌系表现抗病，而对GD-V表现感病，3个MAGIC群体的病斑长度均出现超亲分离。共检测到7个白叶枯病抗性QTL，大多表现数量抗性，而且抗性QTL表达存在明显的遗传背景效应。QBbr11-1和QBbr11-2受遗传背景影响较小，具有一定的育种应用价值。从3个群体筛选出8份不同抗病QTL聚合的抗病材料，表明质量抗性基因和水平抗性数量性状位点的结合可以显著提高抗性水平。8份不同抗病QTL的聚合系可以用作抗病育种的中间抗源。研究结果表明，MAGIC群体可以将遗传研究和育种应用有机结合，是遗传研究和开展标记辅助育种的理想群体。

关键词: MAGIC, 水稻白叶枯病, QTL, 全基因组关联分析, 水稻

Abstract:

Three genetically interconnected multi-parents advanced generation inter-cross, (MAGIC), including two populations (DC1 and DC2) derived from four parents and one population from eight parents (DC3) were used to detect QTLs for resistance to two strains, a weak virulent C2 and a strong virulent GD-V of Xanthomonas oryzae pv. oryzae (Xoo) and to screen resistant breeding materials. Most parents were resistant to C2 and susceptible to GD-V. Transgressive segregations of lesion length for the two strains were observed in the three MAGIC populations and showed continuous distributions. A total of seven QTLs affecting lesion length of two strains were detected. Most QTLs showed quantitative resistance and obvious genetic background effect. Among the seven QTLs, QBbr11-1 and QBbr11-2 had less genetic background effect, which is valuable in rice breeding for disease resistance. Eight resistant lines pyramiding different QTLs were screened from the three MAGIC populations, indicating the combination of qualitative resistance gene and quantitative resistance gene can significantly improve resistance level. The eight resistant breeding lines could be used as resistant donors in rice breeding for resistance. The results indicated that the MAGIC populations are ideal material for genetic study and marker-assisted breeding, showing a tight integration of genetic research and breeding application in rice.

Key words: Multi-parentAdvancedGenerationInter-Crosses(MAGIC), Ricebacterialblight, Quantitativetraitloci(QTL), Genome-wideassociationstudy(GWAS), Rice

陈天晓,朱亚军,密雪飞,陈凯,孟丽君,左示敏,徐建龙. 利用水稻MAGIC群体关联定位白叶枯病抗性QTL和创制抗病新种质[J]. 作物学报, 2016, 42(10): 1437-1447.

CHEN Tian-Xiao1,2, ZHU Ya-Jun3, MI Xue-Fei3, CHEN Kai3, MENG Li-Jun3, ZUO Shi-Min1,*, and XU Jian-Long2,3,4,*. Mapping of QTLs for Bacterial Blight Resistance and Screening of Resistant Materials Using MAGICP opulations of Rice[J]. Acta Agron Sin, 2016, 42(10): 1437-1447.

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链接本文: https://zwxb.chinacrops.org/CN/

https://zwxb.chinacrops.org/CN/Y2016/V42/I10/1437

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利用水稻MAGIC群体关联定位白叶枯病抗性QTL和创制抗病新种质

Mapping of QTLs for Bacterial Blight Resistance and Screening of Resistant Materials Using MAGICP opulations of Rice

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