欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2016, Vol. 42 ›› Issue (10): 1479-1486.doi: 10.3724/SP.J.1006.2016.01479

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黄淮大豆主产区大豆胞囊线虫生理小种分布调查

练云1,**,王金社1,**,李海朝1,魏荷1,李金英1,武永康1,雷晨芳1,张辉1,王树峰1,郭建秋2,李月霞2,李志辉3,靳巧玲3,徐淑霞4,张志民4,杨彩云5,于会勇5,耿臻6,舒文涛6,卢为国1,*   

  1. 1河南省农业科学院经济作物研究所/国家大豆改良中心郑州分中心/农业部黄淮海油料作物重点实验室，郑州450002；2洛阳农林科学院，河南洛阳471023；3漯河市农业科学院，河南漯河462300；4安阳市农业科学院，河南安阳455000；5濮阳市农业科学院，河南濮阳457000；6周口市农业科学院，河南周口466001
  • 收稿日期:2016-03-01 修回日期:2016-06-20 出版日期:2016-10-12 网络出版日期:2016-07-04
  • 通讯作者: 卢为国,E-mail:123bean@163.com
  • 基金资助:

    本研究由国家自然科学基金项目(31371652)，河南省农业科学院科研发展专项资金项目(20137905)，国家现代农业产业技术体系建设专项(CARS-004)和河南省科技攻关项目(162102110141)资助。

Race Distribution of Soybean Cyst Nematode in the Main Soybean Producing Area of Huang-Huai Valleys

LIAN Yun1,??, WANG Jin-She1,??, LI Hai-Chao1, WEI He1, LI Jin-Ying1, WU Yong-Kang1, LEI Chen-Fang1, ZHANG Hui1, WANG Shu-Feng1, GUO Jian-Qiu2, LI Yue-Xia2, LI Zhi-Hui3, JIN Qiao-Ling3, XU Shu-Xia4, ZHANG Zhi-Min4, YANG Cai-Yun5, YU Hui-Yong5, GENG Zhen6, SHU Wen-Tao6, and LU Wei-Guo1,?   

  1. 1 Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huang-Huai Valleys of Ministry of Agriculture / Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 Luoyang Academy of Agriculture and Forestry Sciences, Luoyang 471023, China; 3 Luohe Academy of Agricultural Sciences, Luohe 462300, China; 4 Anyang Academy of Agricultural Sciences, Anyang 455000, China; 5 Puyang Academy of Agricultural Sciences, Puyang 457000, China; 6 Zhoukou Academy of Agricultural Sciences, Zhoukou 466001, China
  • Received:2016-03-01 Revised:2016-06-20 Published:2016-10-12 Published online:2016-07-04
  • Contact: Lu weiguo,E-mail:123bean@163.com
  • Supported by:

    ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina(31371652),theSpecialFundforScientificResearchandDevelopmentofHenanAcademyofAgriculturalSciences(20137905),theChinaAgricultureResearchSystem(CARS-004),andtheScienceandTechnologyProjectofHenanProvince(162102110141).

RichHTML

PDF

701

被引次数

2

摘要：

大豆胞囊线虫(SCN)在黄淮地区普遍发生，调查小种分布情况，确定优势小种对抗病育种有重要意义。2012—2015年，取样调查黄淮地区6个省份土样，利用Riggs模式鉴定生理小种，绘制黄淮地区SCN生理小种分布图，并与文献报道结果对比，探讨黄淮地区SCN生理小种类型及其分布规律。结果表明，该病害在黄淮大豆主产区均有分布，在采集受SCN感染的322份土样中，112份被鉴定出生理小种类型，包括1号、2号、3号、4号、5号、6号和11号小种。其中，57份为2号小种，占样本总体的50.9%；26份土样为5号小种，占23.2%；11份土样为4号小种，占9.8%，1号、3号、6号和11号小种分别占总体的4.5%、5.4%、4.5%和1.8%。依据不同生理小种在各省发生频率由高到低的顺序，河南分布5号、2号、3号、11号小种；河北分布2号、5号、6号、3号、4号小种；安徽分布2号、5号、6号、3号小种；山西分布2号、4号、5号、1号、3号、11号小种；山东分布2号、3号、5号、1号、6号小种；江苏分布2号、5号、1号小种。以上结果表明，2号小种是目前黄淮海地区的优势小种，其次是5号小种，致病力最强的4号小种主要分布在山西省。在黄淮海地区，抗线虫育种目标应以抗2号生理小种为主，兼抗5号小种，部分地区应以兼抗2号和4号小种为主。在黄淮地区3号、6号和11号小种是新发现的小种。与2001-2003年调查结果比较，黄淮海地区大豆胞囊线虫生理小种组成及分布有一定的改变。

关键词: 大豆, 大豆胞囊线虫, 黄淮海地区, 生理小种

Abstract:

Soybean cyst nematode(SCN)(Heterodera glycines), is the most economically important pathogen of soybean in Huang-Huai Valleys, development of resistant cultivars is the most convenient method to avoid yield losses. Knowledge of SCN race distribution in soybean production area is meaningful for the breeding of resistant lines. A 4-year systematic survey for the presence of soybean cyst nematode Heterodera glycines in Huang-Huai Valleys was initiated in 2012. A distribution map of races was constructed based on Riggs model. The race variation law and reason were also discussed in this article. A total of 322 soil samples infected by soybean cyst nematode were collected. Among them, seven races of H. glycines were identified in 112 (34.8%) samples, including five races described previously. Heterodera glycines presented in the six provinces including Henan, Hebei, Anhui, Shanxi, Shandong, and Jiangsu. Among the 112 soil samples mentioned above, race 2 accounted for 50.9% of the identified H. glycines races. Races 5, 4, 3, 1, 6, and 11 did 23.2%, 9.8%, 5.4%, 4.5%, 4.5%, and 1.8%, respectively. In order of frequency from high to low, races 5, 2, 3, 11 distributed in Henan, with race 5 and race 2 accounting for 87.5% of identified H. glycines populations; races 2, 5, and 6 accounted for 92% in Hebei and 95.2% in Anhui; races 2, 3, 5 did 81.86% in Shandong; races 2, 5, 1 distributed in Jiangsu, with race 2 and race 5 accounting for 84.6%; race 2 and race 4 each accounted for 38.5% of the total identified races in Shanxi soybean productin area. Race 2 was the predominant race in Huang-Huai Valleys at present, which should be focused on developing resistant cultivars, followed by race 5. Race 4, the most virulent form of SCN, whose distribution was centered in Shanxi. In some local area breeding should be focused on cultivars resistant to race 2 and race 5 while in some other areas it should be focused on breeding cultivars resistant to race 2 and race 4, according to the race types identified from the experiment and the distribution in specific areas. Races 3, 6, and 11 were the first reported in Huang-Huai Valleys. Compared with the survey conducted at Huang-Huai Valleys in the years of 2001–2003, there were some changes in the race types and distribution in the present surveys.

Key words: Soybean, SoybeanCystNematode, Huang-HuaiValleys, Race

[1]WratherJA,KoenningSR.EstimatesofdiseaseeffectsonsoybeanyieldsintheUnitedStates2003to2005.JNematol,2006,38:173–180 [2]NiblackTL,TylkaGL,RiggsRD.Nematodepathogensofsoybean.WilcoxJR.Soybeans:Improvement,Production,andUses.Madison,WI,USA:Americansocietyofagronomy,cropsciencesocietyofAmerica,andsoilsciencesocietyofAmerica,2004.pp821–851 [3]NiblackTL,WratherJA,HeinzRD,DonaldPA.DistributionandvirulencephenotypesofHeteroderaglycinesinMissouri.PlantDis,2003,87(8):929–932 [4]WillsonHR,RiedelRM,EisleyJB,YoungCE,JasinskiJR,WheelerTA,KauffmanPH,PiersonPE,StuartMC.DistributionandvirulencephenotypesofHeteroderaglycinesinMissouri.JNematol,1996,28:599–603 [5]MitchumMG,WratherJA,HeinzRD,ShannonJG,DanekasG.VariabilityindistributionandvirulencephenotypesofHeteroderaglycinesinMissouriduring2005.PlantDis,2007,91:1473–1476 [6]何艳琴,闫晓燕,吴存祥,杨中路,年海.中国大豆新品种动态.北京:中国农业科学技术出版社,2013 HeYQ,YanXY,WuCX,YangZL,NianH.ThedynamicofnewsoybeanvarietyinChina.Beijing:ChinaAgriculturalScienceandTechnologyPress,2013 [7]练云,卢为国.大豆抗SCN机制及抗病相关基因研究进展.中国油料作物学报,2013,35:727–732 LianY,LuWG.AdvancesonresistancemechanismandgenetoSCNinsoybean.ChinJOilCropSci,2013,35:727–732(inChinesewithEnglishabstract) [8]卢为国,盖钧镒,李卫东.黄淮地区大豆胞囊线虫(HeteroderaglycinesIchinohe)生理小种的抽样调查与研究.中国农业科学,2006,39:306–312 LuWG,GaiJY,LiWD.Samplingsurveyandidentificationofracesofsoybeancystnematode(HeteroderaglycinesIchinohe)inHuang-HuaiValleys.SciAgricSin,2006,39:306–312(inChinesewithEnglishabstract) [9]张磊.安徽淮北地区大豆胞囊线虫生理小种研究初报.大豆科学,1988,7:251–254 ZhangL.Preliminaryresearchonracesofsoybeancystnematode(HeteroderaglycinesIchinohe)inHuaibeiareaofAnhuiprovince.SoybeanSci,1988,7:251-254 [10]陈品三,张东生,陈森玉.大豆胞囊线虫7号生理小种的研究初报.中国农业科学,1987,20(2):94 ChenPS,ZhangDS,ChenSY.Firstreportonanewphysiologicalrace(race7)ofsoybeancystnematode(Heteroderaglycines).SciAgricSin,1987,20(2):94 [11]刘汉起,商邵刚,甄鸿杰,霍虹,吴和礼,姚振纯,李秀兰.大豆胞囊线虫生理小种研究初报.大豆科学,1985,4:131–136 LiuHQ,ShangSG,ZhenHJ,HuoH,WuHL,YaoZC,LiXL.Studyonphysiologicalracesofsoybeancystnematode(Heteroderaglycines).SoybeanSci,1985,4:131–136(inChinesewithEnglishabstract) [12]李卫东,贺春林,田保明.河南省大豆胞囊线虫分布及生理小种鉴定.华北农学报,1991,6(增刊1):111–114 LiWD,HeCL,TianBM.Samplingsurveyandidentificationofracesofsoybeancystnematode(HeteroderaglycinesIchinohe)intheprovinceofHenan.ActaAgricBoreali-Sin,1991,6(S1):111–114 [13]邢邯,赵经荣,战明奎,李佩廷,盖钧镒.山东省大豆胞囊线虫生理小种的鉴定.中国油料作物学报,1997,19(4):61–65 XingH,ZhaoJR,ZhanMK,LiPT,GaiJY.Identificationofracesofsoybeancystnematode(HeteroderaglycinesIchinohe)fromShandongprovince.OilCropsChina,1997,19(4):61–65(inChinesewithEnglishabstract) [14]李莹,王志,焦广音,常汝镇.中国大豆遗传资源对大豆胞囊线虫4号生理小种的抗性鉴定研究.中国农业科学,1991,24(5):64–69 LiY,WangZ,JiaoGY,ChangRZ.Studiesonresistanceofsoybeangermplasmresourcestorace4ofsoybeancystnematode.SciAgricSin,1991,24(5):64–69(inChinesewithEnglishabstract) [15]王衍桐,彭德良,陈受宜.灰布支黑豆对大豆孢囊线虫(Heteroderaglycines)14号小种的抗性遗传.遗传学报,2000,27:146–150 WangYT,PengDL,ChenSY.InheritanceofresistancetoHeteroderaglycinesrace14inHuibuzhiblackbean.ActaGenetSin,2000,27:146–150(inChinesewithEnglishabstract) [16]RiggsRD,SchmittDP.CompletecharacterizationoftheraceschemeforHeteroderaglycines.JNemato,1988,20:392–395 [17]练云,魏荷,王金社,雷晨芳,李海朝,武永康,卢为国.影响大豆胞囊线虫生理小种鉴定因素探讨.分子植物育种,2015,13:1259–1264 LianY,WeiH,WangJS,LeiCF,LiHC,WuYK,LuWG.Astudyonthefactorsthatinfluencetherace-identificationofsoybeancystnematode.MolPlantBreed,2015,13:1259–1264(inChinesewithEnglishabstract) [18]王金社,卢为国,李金英,练云,魏荷,李海朝,雷晨芳.植物病虫害表型数据采集系统.专利号:2014SR060158,中国,2014 WangJS,LuWG,LiJY,LianY,WeiH,LiHC,LeiCF.Thedataacquisitionsystemonthephenotypeofplantdiseasesandinsectpests.Patentnumber:2014SR060158,China,2014 [19]TeamRC.R:Alanguageandenvironmentforstatisticalcomputing.RFoundStatistComput,2014 [20]NiblackTL,ArelliPR,NoelGR,OppermanCH,OrfJH,SchmittDP,ShannonJG,TylkaGL.ArevisedclassificationschemeforgeneticallydiversepopulationsofHeteroderaglycines.JNematol,2002,34:279–288 [21]AfzalAJ,SrourA,SainiN,HemmatiN,ElShemyHA,LightfootDA.RecombinationsuppressionatthedominantRhg1/Rfs2locusunderlyingsoybeanresistancetothecystnematode.TAG,2011,124:1027–1039 [22]YuanCP,LiYH,LiuZX,GuanRX,ChangRZ,QiuLJ.DNAsequencepolymorphismoftheRhg4candidategeneconferringresistancetosoybeancystnematodeinChinesedomesticatedandwildsoybeans.MolBreed,2012,30:1155–1162 [23]GloverKD,WangD,ArelliPR,CarlsonSR,CianzioSR,DiersBW.NearisogeniclinesconfirmasoybeancystnematoderesistancegeneromPI88788onlinkagegroup.JCropSci,2004,44:936–941 [24]VuongTD,SleperDA,ShannonJG,NguyenHT.Novelquantitativetraitlociforbroad-basedresistancetosoybeancystnematode(HeteroderaglycinesIchinohe)insoybeanPI567516C.ThoerApplGenet,2010,121:1253–1266 [25]LiuX,LiuS,JamaiA,BendahmaneA,LightfootDA,MitchumMG,MeksemK.SoybeancystnematoderesistanceinsoybeanisindependentoftheRhg4locusLRR-RLKgene.FunctIntegrGenomics,2011,11:539–549 [26]卢为国,盖钧镒,郑永战,李卫东.大豆遗传图谱的构建和抗胞囊线虫(HeteroderaglycinesIchinohe)的QTL分析.作物学报,2006,32:1272–1279 LuWG,GaiJY,ZhengYZ,LiWD.ConstructionofasoybeangeneticlinkagemapandmappingQTLsresistanttosoybeancystnematode(HeteroderaglycinesIchinohe).ActaAgronSin,2006,32:1272–1279(inChinesewithEnglishabstract) [27]ConcibidoVC,DiersBW,ArelliPR.AdecadeofQTLmappingforcystnematoderesistanceinsoybean.CropSci,2004,44:1121–1131 [28]AfzalAJ,NatarajanA,SainiN,IqbalMJ,GeislerM,ElShemyHA,MungurR,WillmitzerL,LightfootDA.Thenematoderesistancealleleattherhg1locusalterstheproteomeandprimarymetabolismofsoybeanroots.PlantPhysiol,2009,151:1264–1280
[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[3] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[4] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[5] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[6] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[7] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[8] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[9] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[10] 周悦, 赵志华, 张宏宁, 孔佑宾. 大豆紫色酸性磷酸酶基因GmPAP14启动子克隆与功能分析[J]. 作物学报, 2022, 48(3): 590-596.
[11] 王娟, 张彦威, 焦铸锦, 刘盼盼, 常玮. 利用PyBSASeq算法挖掘大豆百粒重相关位点与候选基因[J]. 作物学报, 2022, 48(3): 635-643.
[12] 董衍坤, 黄定全, 高震, 陈栩. 大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能[J]. 作物学报, 2022, 48(2): 353-366.
[13] 张国伟, 李凯, 李思嘉, 王晓婧, 杨长琴, 刘瑞显. 减库对大豆叶片碳代谢的影响[J]. 作物学报, 2022, 48(2): 529-537.
[14] 宋丽君, 聂晓玉, 何磊磊, 蒯婕, 杨华, 郭安国, 黄俊生, 傅廷栋, 汪波, 周广生. 饲用大豆品种耐荫性鉴定指标筛选及综合评价[J]. 作物学报, 2021, 47(9): 1741-1752.
[15] 曹亮, 杜昕, 于高波, 金喜军, 张明聪, 任春元, 王孟雪, 张玉先. 外源褪黑素对干旱胁迫下绥农26大豆鼓粒期叶片碳氮代谢调控的途径分析[J]. 作物学报, 2021, 47(9): 1779-1790.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2