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作物学报 ›› 2016, Vol. 42 ›› Issue (10): 1487-1494.doi: 10.3724/SP.J.1006.2016.01487

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米氮素敏感性差异自交系的表达谱分析

葛敏,吕远大,张体付,周玲,林峰,赵涵*   

  1. 江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室,江苏南京210014
  • 收稿日期:2016-02-22 修回日期:2016-06-20 出版日期:2016-10-12 网络出版日期:2016-07-28
  • 通讯作者: 赵涵,E-mail:zhaohan@jaas.ac.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31271728)和江苏省农业科技自主创新资金项目(CX(14)2009)资助。

Global Transcriptome Analysis in High- and Low-Nitrogen Responsive Inbred Lines of Maize

GE Min, LYU Yuan-Da, ZHANG Ti-Fu, ZHOU Ling, LIN Feng, and ZHAO Han*   

  1. Institute of Biotechnology, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2016-02-22 Revised:2016-06-20 Published:2016-10-12 Published online:2016-07-28
  • Contact: Zhao Han,E-mail:zhaohan@jaas.ac.cn
  • Supported by:

    ThisworkwassupportedbygrantfromtheNaturalScienceFoundationofChina(31271728)andJiangsuAgricultureScienceandTechnologyInnovationFund[CX(14)2009].

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摘要：

玉米材料的氮肥敏感性存在显著差异，但基因表达模式尚不清楚。基于此，本研究以玉米氮敏感型自交系 B73和钝感型自交系Mo17为材料，对足氮(sufficient nitrogen，简称SN)和低氮(limiting nitrogen，简称LN)条件下苗期叶片组织的转录组进行分析。对于叶片总氮含量，敏感型B73在足氮和低氮条件下存在显著差异，而钝感型Mo17的差异小且不显著。基因表达差异分析显示Mo17在两种氮环境下差异基因的数目达13 867个，在低氮环境下基因上调比例高于下调比例1.9倍；B73差异基因的数目为10 028个，低氮环境基因上调比例低于下调比例。基因聚类分析也显示低氮环境下，钝感型Mo17基因表达上调或下调的幅度高于敏感型B73。差异基因双尾方差分析表明受氮环境和基因型共同影响的差异基因为342个，功能主要集中在与氨基酸代谢、光合作用、次级代谢及基因复制表达等相关途径。综上所述，在低氮条件下氮钝感型Mo17较敏感型B73激活更多的基因来提高植株对氮的吸收和同化能力，被激活的基因可能与玉米氮肥转运和利用有关。

关键词: 玉米, 氮素, 氮胁迫, 转录组分析, 氮素利用率

Abstract:

Different maize genotypes have exhibited their variations in nitrogen responsiveness, however, the corresponding gene expression patterns are unexplored yet. Here, we performed a comprehensive transcriptome profiling of high- and low-nitrogen responsiveness genotypes in the conditions of sufficient and limiting nitrogen (SN and LN) supplies. Under LN supply, B73, a high nitrogen responsive genotype, accumulated a much lower nitrogen content than under SN condition in the leaf. Meanwhile, the N content of Mo17 didn’t show a significant difference between two treatments. Furthermore, RNAseq revealed that 13 867 and 10 028 genes were significantly differentially expressed in Mo17 and B73 with the treatments, with 9044, 4233 up-regulated and 4823, 5795 down-regulated under N limitation. Showing that, in LN supply, Mo17 had more expressed genes and higher expression variations than B73. A total of 342 differentially expressed genes with a significant interaction genotype by nitrogen were detected, which could be clustered into four groups. The functional annotations of genes demonstrated that these genes were mainly involved in amino acid metabolism, photosynthesis and biosynthesis of secondary metabolites, suggesting these genes may have their roles in modulating maize nitrogen use efficiency. The study promotes a better understanding of the mechanisms on maize nitrogen response, and provides candidate genes for breeding of crop nitrogen use efficiency in the future.

Key words: Maize, Nitrogen, Nitrogenstress, Transcriptomeprofiling, Nitrogenuseefficiency(NUE)

[1]GutierrezRA.Systemsbiologyforenhancedplantnitrogennutrition.Science,2012,336:1673–1675 [2]MarchiveC,RoudierF,CastaingsL,BrehautV,BlondetE,ColotV,MeyerC,KrappA.NuclearretentionofthetranscriptionfactorNLP7orchestratestheearlyresponsetonitrateinplants.NatCommun,2013,4:1713 [3]BiYM,MeyerA,DownsGS,ShiX,El-KereamyA,LukensL,RothsteinSJ.HighthroughputRNAsequencingofahybridmaizeanditsparentsshowsdifferentmechanismsresponsivetonitrogenlimitation.BMCGenomics,2014,15:77 [4]HumbertS,SubediS,CohnJ,ZengB,BiYM,ChenX,ZhuT,McNicholasPD,RothsteinSJ.Genome-wideexpressionprofilingofmaizeinresponsetoindividualandcombinedwaterandnitrogenstresses.BMCGenomics,2013,14:3 [5]HvistendahlM.China'spushtoaddbysubtractingfertilizer.Science,2010,327:801 [6]MollR,KamprathE,JacksonW.Analysisandinterpretationoffactorswhichcontributetoefficiencytonitrogenutilization.AgronJ,1982,74:562–564 [7]YamayaT,ObaraM,NakajimaH,SasakiS,HayakawaT,SatoT.Geneticmanipulationandquantitativetraitlocimappingfornitrogenrecyclinginrice.JExpBot,2002,53:917–925 [8]ObaraM,SatoT,SasakiS,KashibaK,NaganoA,NakamuraI,EbitaniT,YanoM,YamayaT.IdentificationandcharacterizationofaQTLonchromosome2forcytosolicglutaminesynthetasecontentandpaniclenumberinrice.TheorApplGenet,2004,110:1–11 [9]ObaraM,TamuraW,EbitaniT,YanoM,SatoT,YamayaT.Fine-mappingofqRL6.1,amajorQTLforrootlengthofriceseedlingsgrownunderawiderangeofNH4+concentrationsinhydroponicconditions.TheorApplGenet,2010,121:535–547 [10]SunH,QianQ,WuK,LuoJ,WangS,ZhangC,MaY,LiuQ,HuangX,YuanQ,HanR,ZhaoM,DongG,GuoL,ZhuX,GouZ,WangW,WuY,LinH,FuX.HeterotrimericGproteinsregulatenitrogen-useefficiencyinrice.NatGenet,2014,46:652–656 [11]HuangX,QianQ,LiuZ,SunH,HeS,LuoD,XiaG,ChuC,LiJ,FuX.NaturalvariationattheDEP1locusenhancesgrainyieldinrice.NatGenet,2009,41:494–497 [12]AgramaHA,MoussaME,NaserME,TarekMA,IbrahimAH.MappingofQTLfordownymildewresistanceinmaize.TheorApplGenet,1999,99:519–523 [13]CoqueM,GallaisA.Genomicregionsinvolvedinresponsetograinyieldselectionathighandlownitrogenfertilizationinmaize.TheorApplGenet,2006,112:1205–1220 [14]CoqueM,MartinA,VeyrierasJB,HirelB,GallaisA.GeneticvariationforN-remobilizationandpostsilkingN-uptakeinasetofmaizerecombinantinbredlines.3.QTLdetectionandcoincidences.TheorApplGenet,2008,117:729–747 [15]米国华,陈范骏,春亮,郭亚芬,田秋英,张福锁.玉米氮高效品种的生物学特征.植物营养与肥料学报,2007,13:155–159 MiGH,ChenFJ,ChuaL,GouYF,TianQY,ZhangFS.Biologicalcharacteristicsofnitrogenefficientmaizegenotypes.PlantNutrFertSci,2007,13:155–159(inChinesewithEnglishabstract) [16]ChenQY,LiuZP,WangBB,WangXF,LaiJS,TianF.Transcriptomesequencingrevealstherolesoftranscriptionfactorsinmodulatinggenotypebynitrogeninteractioninmaize.PlantCellRep,2015,34:1761–1771 [17]GelliM,DuoYC,KondaAR,ZhangC,HoldingD,DweikatI.Identificationofdifferentiallyexpressedgenesbetweensorghumgenotypeswithcontrastingnitrogenstresstolerancebygenome-widetranscriptionalprofiling.BMCGenomics,2014,15:179 [18]SchlüterU,MascherM,ColmseeC,ScholzU,Br?utigamA,FahnenstichH,SonnewaldU.Maizesourceleafadaptationtonitrogendeficiencyaffectsnotonlynitrogenandcarbonmetabolismbutalsocontrolofphosphatehomeostasis.PlantPhysiol,2012,160:1384–1406. [19]葛敏,吕远大,李坦,张体付,张晓林,赵涵.玉米Dof转录因子家族的全基因组鉴定与分析.中国农业科学,2014,47:4563–4572 GeM,LüYD,LiT,ZhangTF,ZhangXL,ZhaoH.Genome-wideidentificationandanalysisofDoftranscriptionfactorfamilyinmaize.SciAgricSin,2014,47:4563–4572(inChinesewithEnglishabstract) [20]PattersonN,PriceA,ReichD.Populationstructureandeigenanalysis.PLoSGenet,2006,2(12):e190 [21]WidiezT,ElKafafielS,GirinT,BerrA,RuffelS,KroukG,VayssièresA,ShenWH,CoruzziGM,GojonA,LepetitM.Highnitrogeninsensitive9(HNI9)-mediatedsystemicrepressionofrootNO3-uptakeisassociatedwithchangesinhistonemethylation.ProcNatlAcadSciUSA,2011,108:13329–13334 [22]XieC,MaoX,HuangJ,DingY,WuJ,DongS,KongL,GaoG,LiCY,WeiL.KOBAS2.0:awebserverforannotationandidentificationofenrichedpathwaysanddiseases.NuclAcidsRes,2011,39:W316–W322 [23]YangXS,WuJ,ZieglerTE,YangX,ZayedA,RajaniMS,ZhouD,BasraAS,SchachtmanDP,PengM,ArmstrongCL,CaldoRA,MorrellJA,LacyM,StaubJM.Geneexpressionbiomarkersprovidesensitiveindicatorsofinplantanitrogenstatusinmaize.PlantPhysiol,2011,157:1841–1852 [24]LiuKH,HuangCY,TsayYF.CHL1isadual-affinitynitratetransporterofArabidopsisinvolvedinmultiplephasesofnitrateuptake.PlantCell,1999,11:865–874 [25]MartinA,LeeJ,KicheyT,GerentesD,ZivyM,TatoutC,DuboisF,BalliauT,ValotB,DavantureM,Terce-LaforgueT,QuillereI,CoqueM,GallaisA,Gonzalez-MoroMB,BethencourtL,HabashDZ,LeaPJ,CharcossetA,PerezP,MurigneuxA,SakakibaraH,EdwardsKJ,HirelB.Twocytosolicglutaminesynthetaseisoformsofmaizearespecificallyinvolvedinthecontrolofgrainproduction.PlantCell,2006,18:3252–3274 [26]BrauerEK,RochonA,BiYM,BozzoGG,RothsteinSJ,ShelpBJ.Reappraisalofnitrogenuseefficiencyinriceoverexpressingglutaminesynthetase1.PhysiolPlant,2011,141:361–372 [27]CastaingsL,CamargoA,PocholleD,GaudonV,TexierY,Boutet-MerceyS,TaconnatL,RenouJP,Daniel-VedeleF,FernandezE,MeyerC,KrappA.Thenoduleinception-likeprotein7modulatesnitratesensingandmetabolisminArabidopsis.PlantJ,2009,57:426–435 [28]ChardinC,GirinT,RoudierF,MeyerC,KrappA.TheplantRWP-RKtranscriptionfactors:keyregulatorsofnitrogenresponsesandofgametophytedevelopment.JExpBot,2014,65:5577–5587
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