作物学报 ›› 2016, Vol. 42 ›› Issue (11): 1609-1619.doi: 10.3724/SP.J.1006.2016.01609
童治军,焦芳婵,方敦煌,陈学军,吴兴富,曾建敏,谢贺,张谊寒,肖炳光*
TONG Zhi-Jun, JIAO Fang-Chan, FANG Dun-Huang, CHEN Xue-Jun, WU Xing-Fu, ZENG Jian-Min, XIE He, ZHANG Yi-Han, and XIAO Bing-Guang*
摘要:
以综合性状优良的烤烟种质Y3为轮回亲本,抗烟草黑胫病(0、1号生理小种)和赤星病的雪茄烟种质Beinhart1000-1及优质烤烟品种K326为供体亲本,连续回交并结合分子标记辅助选择,构建国内外第一套由256个具有烤烟Y3遗传背景并渐渗有Beinhart1000-1和K326染色体片段株系代换系群体。该群体携带377个代换片段,分布于烟草24条连锁群上。每个株系携带1~5个代换片段,代换片段长度介于0.05~36.88 cM,平均长度7.75 cM。代换片段重叠累加总长度为2922.57 cM,是烟草基因组总长度的2.61倍。代换片段覆盖总长度为1114.32 cM,烟草基因组覆盖率为99.45%。本研究构建的片段代换系可用于烟草基因定位、复杂性状的QTL分析和标记辅助选择育种。
[1]王元英,周健.中美主要烟草品种亲源分析与烟草育种.中国烟草学报,1995,13(5):11–22 WangYY,ZhouJ.GeneticanalysisofthemaintobaccocultivarsandthetobaccobreedinginUSAandChina.ActaTabSin,1995,13(5):11–22(inChinesewithEnglishabstract) [2]TongZJ,JiaoTL,WangFQ,LiMY,LenXD,GaoYL,LiYP,XiaoBG,WuWR.Mappingofquantitativetraitlociconferringresistancetobrownspotinflue-curedtobacco(NicotianatabacumL.).PlantBreed,2012,131:335–339 [3]TongZJ,JiaoFC,WuXF,WangFQ,ChenXJ,LiXY,GaoYL,ZhangYH,XiaoBG,WuWR.Mappingofquantitativetraitlociunderlyingsixagronomictraitsinflue-curedtobacco(NicotianatabacumL.).ActaAgronSin,2012,38:1–9 [4]NishiT,TajimaT,NoguchiS,AjisakaH,NegishiH.IdentificationofDNAmarkersoftobaccolinkedtobacterialwiltresistance.TheorApplGenet,2003,106:765–770 [5]JulioE,DenoyesRB,VerrierJL,deBorneFD.DetectionofQTLslinkedtoleafandsmokepropertiesinNicotianatabacumbasedonastudyof114recombinantinbredlines.MolBreed,2006,22:144–166 [6]VontimittaV,LewisRS.MappingofquantitativetraitlociaffectingresistancetoPhytophthoranicotianaeintobacco(NicotianatabacumL.)lineBeihart-1000.MolBreed,2010,58:294–300 [7]廖长见,王颖妲,潘光堂.作物染色体导入系的构建及其应用.分子植物育种,2007,5(6):139–144 LiaoCJ,WangYH,PanGT.Constructionandapplicationofchromosomeintrogressionlinesincrops.MolPlantBreed,2007,5(6):139–144(inChinesewithEnglishabstract) [8]JenaKK,KhushGS,KochertG.RFLPanalysisofrice(Oryzasatival)introgressionlines.TheorApplGenet,1992,84:608–616 [9]EshedY,AbiedM,SarangaY,ZamirD.Lycopersiconesculentumlinescontainingsmalloverlappingintrogressionsfrompennellii.TheorApplGenet,1992,83:1027–1034 [10]EshedY,ZamIR.AnintrogressionlinepopulationofLycopersiconpennelliiinthecultivatedtomatoenablestheidentificationandfinemappingofyield-associatedQTL.Genetics,1995,14:1147–1162 [11]MonforteAJ,TanksleySD.DevelopmentofasetofnearisogenicandbackcrossrecombinantinbredlinescontainingmostoftheLycopersiconhirsutumgenomeinanL.esculentumgeneticbackground:Atoolforgenemappingandgenediscovery.Genome,2000,43:803–813 [12]BernacehiD,Beck-BunnT,EmmattyD,EshedY,InaiS,LoPezJ,PetiardV,SayamaH,U'hligJ,ZamirD,TanksleyS.AdvancedbackcrossQTLanalysisoftomato.II.Evaluationofnear-isogeniclinescarryingsingle-donorintrogressionsfordesirablewildQTL-allelesderivedfromLycopersiconhirsutumandL.pimpinellifolium.TheorApplGenet,1998,97:170–180 [13]RamsayLD,JenningsDE,BohuonEJR,ArihurAE,LydiateDJ,KearseyMJ,MarshallDF.TheconstructionofasubstitutionlibraryofrecombinantbackcrosslinesinBrassicaoleraceafortheprecisionmappingofquantitativetraitloci.Genome,1996,39:558–567 [14]JeukenMJW,LindhoutP.Thedevelopmentoflettucebackcrossinbredlines(BILs)forexploitationofthelaetucasaligna(wildlettuce)germplasm.TheroApplGenet,2004,109:394–401 [15]MatusL,CoreyA,FiliehkinT.Developmentandcharacterizationofrecombinantchromosomesubstitutionlines(RCSLs)usingHordeumvelgaresubsp.spontaneumasasourceofdonorallelesinaHordeumvulgarsubsp.velgarebackground.Genome,2003,46:10l0–1023 [16]KorffMV,WangH,LeonJ.Developmentofcandidateintrogressionlinesusinganexoticbarleyaccession(Hordeumvulgarsubsp.spontaneous)asdonor.TheorApplGenet,2004,109:1736–1745 [17]冯长辉,张胜昔,王志伟,王夏青,李夕梅.棉花染色体单片段导入系的研究进展.湖北农业科学,2009,48(12):24–26 FengCH,ZhangSX,WangZW,WangXQ,LiXM.ResearchprogressonconstructionandapplicationofSSILsincotton.HubeiAgriculturalSciences,2009,48(12):24–26(inChinesewithEnglishabstract) [18]孟佳丽,娄群峰,周晓慧,史建磊,陈劲枫.黄瓜-酸黄瓜染色体片段导入系群体的构建及果实相关数量性状基因的定位.中国农业科学,2012,45(8):1558–1567 MengJL,LouQF,ZhouXH,ShiJL,ChenJF.Constructionofcucumber-sourcucumberchromosomeintrogressionlinesandlocationoffruitrelatedQTLs.SciAgricSin,2012,45:1558–1567(inChinesewithEnglishabstract) [19]林肖剑,许学文,钱红梅,齐晓花,徐强,陈学好.黄瓜抗白粉病染色体片段导入系的SSR鉴定.园艺学报,2012,39:485–492 LinJX,XuXW,QianHM,QiXH,XuQ,ChenXH.AnalysisofcucumberchromosomesegmentintrogressionlinewithpowderymildewresistancebasedonSSRmarkers.ActaHortSin,2012,39:485–492(inChinesewithEnglishabstract) [20]史建磊,娄群峰,钱春桃,万红建,周晓慧,陈劲枫.黄瓜染色体片段导入系的构建与遗传评价.南京农业大学学报,2011,34(1):20–24 ShiJL,LouQF,QianCT,WanHJ,ZhouXH,ChenJF.Constructionandgeneticevaluationofchromosomesegmentintrogressionlinesincucumber.JNanjingAgricUniv,2011,34(1):20–24(inChinesewithEnglishabstract) [21]刘冠明,李文涛,曾瑞珍,张泽民,张桂权.水稻单片段代换系片段的QTL鉴定.遗传学报,2004,31:1395–1400 LiuGM,LiWT,ZengRZ,ZhangGQ.IdentificationofQTLsonsubstitutedsegmentsinsinglesegmentsubstitutionlinesofrice.ActaGenetSin,2004,31:1395–1400(inChinesewithEnglishabstract) [22]何风华,席章营,曾瑞珍,AkshayT,张桂权.利用高代回交和分子标记辅助选择建立水稻单片段代换系.遗传学报,2005,32:825–831 HeFH,XiZY,ZengRZ,AkshayT,ZhangGQ.Developingsinglesegmentsubstitutionlines(SSSls)inrice(OryzasativalL)usingadvancedbackcrossesandMAS.ActaGenetSin,2004,32:825–831(inChinesewithEnglishabstract) [23]LiZK,FuBY,GaoYN.Genome-wideintrogressionlinesandtheiruseingeneticandmoleculardissectionofcomplexphenotypesinrice(OryzasativaL.).PlantMolBiol,2005,59:33–52 [24]王玉民,席章营,尚爱兰,王帮太.作物单片段代换系的构建及应用.中国农学通报,2008,24(3):67–71 WangYM,XiZY,ShangAL,WangBT.Constructionandapplicationofsinglesegmentsubstitutionlinesincrops.ChinAgricSciBull,2008,24(3):67–71(inChinesewithEnglishabstract) [25]Bindler,G,derHoevenVR,GunduzI,PlieskeJ,GanalM,RossiL,GadaniF,DoniniP.Amicrosatellitemarkerbasedlinkagemapoftobacco.TheorApplGenet,2007,114:341–349 [26]Bindler,G,PlieskeJ,BakaherN,GunduzI,IvanovN,derHoevenRV,GanalM,DoniniP.Ahighdensitygeneticmapoftobacco(NicotianatabacumL.)obtainedfromlargescalemicrosatellitemarkerdevelopment.TheorApplGenet,2011,123:219–230 [27]Sierro,N,BatteyJND,OuadiS,BakaherN,BovetL,WilligA,GoepfertS,PeitschMC,IvanovNV.Thetobaccogenomesequenceanditscomparisonwiththoseoftomatoandpotato.Nat.Commun,2014,5:3833doi:10.1038/ncomms4833 [28]TongZJ,YangZM,ChenXJ,JiaoFC,LiXY,WuXF,GaoYL,XiaoBG,WuWR.Large-scaledevelopmentofmicrosatellitemarkersinNicotianatabacumandconstructionofageneticmapofflue-curedtobacco.PlantBreed,2012,131:674–680 [29]ArslanB,OkunusA.Geneticandgeographicpolymorphismofcultivatedtobaccos(Nicotianatabacum)inTurkey.PlantGenet,2006,42:667–671 [30]DelPianoL,AbetMC,SorrentinoF,AcanforaE,CozzolinoE,DiMuroA.GeneticvariabilityinNicotianatabacumandNicotianaspeciesasrevealedbyRAPDprocedure.IntlContributTobRes,2000,19:1–15 [31]MoonHS,NicholsonJS,LewisRS.UseoftransferableNicotianatabacumL.microsatellitemarkersforinvestigatinggeneticdiversityinthegenusNicotiana.Genome,2008,51:547–559 [32]MoonHS,NicholsonJS,HeinemanA,LionK,derHoevenRV,HayesAJ,LewisRS.ChangesingeneticdiversityofU.S.Flue-Curedtobaccogermplasmoversevendecadesofcultivardevelopment.CropSci,2009,49:498–506 [33]MoonHS,NifongJM,NicholsonJS,HeinemanA,LionK,derHoevenRV,HayesAJ,LewisRS.Microsatellite-basedanalysisoftobacco(NicotianatabacumL.)geneticresources.CropSci,2009,49:2149–2157 [34]BaiD,ReelederR,BrandleJE.IdentificationoftwoRAPDmarkerstightlylinkedwiththeNicotianadebneyigeneforresistancetoblackrootrotoftobacco.TheorApplGenet,1995,91:1184–1189 [35]MaguireT,CollinsG,SedgleyM.AmodifiedCTABDNAextractionprocedureforplantsbelongingtothefamilyProteaceae.PlantMolBiolRep,1994,12:106–109 [36]YongND,TanlalesySD.Restrictionfragmentpolymorphismmapsandtheconceptofgraphicalgenotypes.TheorApplGenet,1989,77:95–101 [37]张桂权,曾瑞珍,丁效华,张泽民,李文涛.水稻单片段代换系文库的构建及其在分子育种上的应用.见:方宣钧,黎志康主编.2005植物分子育种国际学术研讨会论文集.北京:科学出版社,2005.pp106–113 ZhangGQ,ZengRZ,DingXH,LiWT.Constructionofalibraryofsinglesegmentsubstitutionlinesforricebreedingbydesign.In:FangXJ,LiZK,eds.Proceedingsofthe2005InternationalConferenceonPlantMolecularBreeding.Beijing:SciencePress,2005.pp106-113 |
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