作物学报 ›› 2017, Vol. 43 ›› Issue (07): 974-982.doi: 10.3724/SP.J.1006.2017.00974
周可1,**,李燕1,2,**,王世明1,崔国庆1,杨正林1,何光华1,凌英华1,赵芳明1,*
ZHOU Ke1,**,LI Yan1,2,**,WANG Shi-Ming1,CUI Guo-Qing1,YANG Zheng-Lin1,HE Guang-Hua1,LING Ying-Hua1,ZHAO Fang-Ming1,*
摘要:
植物色素,在食品加工、杂种纯度鉴定中具有重要的作用。本研究鉴定了一个以日本晴为受体、优良紫鞘恢复系R225为供体亲本的紫鞘水稻染色体代换片段系Z519。Z519共含有16个代换片段,分布于除第10染色体外的其他11条染色体,平均长度为6.85 Mb。Z519在芽鞘3 mm时鞘尖呈现紫色,其后在叶鞘、叶缘、茎维管束和柱头等部位出现紫色线条,而日本晴各部位均为绿色。Z519叶鞘中花青素含量极显著高于日本晴,剑叶中没有显著差异。与受体日本晴相比,Z519的株高显著降低,千粒重、主穗总粒数和实粒数显著增加,有效穗数、主穗长和结实率无显著差异。进一步以日本晴与Z519杂交产生的F1和F2隐性群体对紫鞘基因进行了遗传分析和分子定位。该紫鞘表型受显性单基因控制,位于第1染色体InDel标记L03和SSR标记L01之间37.8 kb的区域,被命名为PSH1。对该区间进行候选基因预测和测序,Z519在一个编码质体ATP/ADP转运蛋白的LOC_Os01g45910基因第一外显子的第238~252的GTG重复区又多插入了GTG 3个碱基,导致增加了一个甘氨酸。qRT-PCR结果进一步表明其表达量在Z519中明显降低,初步确定LOC_Os01g45910是PSH1的候选基因。该研究为PSH1调控花青素的分子机制奠定了良好基础。
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