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作物学报

作物学报 ›› 2018, Vol. 44 ›› Issue (8): 1114-1126.doi: 10.3724/SP.J.1006.2018.01114

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

海岛棉CSSLs分子评价及纤维品质、产量性状QTL定位

李超1,2(),李志坤1,谷淇深1,杨君1,柯会锋1,吴立强1,王国宁1,张艳1,吴金华1,张桂寅1,阎媛媛1,马峙英1,王省芬1,*()   

  1. 1 河北农业大学教育部华北作物种质资源研究与利用重点实验室, 河北保定 071001
    2 中国农业科学院棉花研究所 / 棉花生物学国家重点实验室, 河南安阳455000
  • 收稿日期:2017-11-06 接受日期:2018-06-12 出版日期:2018-08-10 网络出版日期:2018-06-20
  • 通讯作者: 王省芬
  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0101006);河北省科技支撑计划项目(16226307D)

Molecular Evaluation for Chromosome Segment Substitution Lines of Gossypium barbadense and QTL Mapping for Fiber Quality and Yield

Chao LI1,2(),Zhi-Kun LI1,Qi-Shen GU1,Jun YANG1,Hui-Feng KE1,Li-Qiang WU1,Guo-Ning WANG1,Yan ZHANG1,Jin-Hua WU1,Gui-Yin ZHANG1,Yuan-Yuan YAN1,Zhi-Ying MA1,Xing-Fen WANG1,*()   

  1. 1 North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, China
    2 Institute of Cotton Research, Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China
  • Received:2017-11-06 Accepted:2018-06-12 Published:2018-08-10 Published online:2018-06-20
  • Contact: Xing-Fen WANG
  • Supported by:
    The study was supported by the National Key Research and Development Program of China(2016YFD0101006);Hebei Science and Technology Support Program(16226307D)

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摘要：

本课题组前期以陆地棉中棉所8号(CCRI8)为轮回亲本, 海岛棉Pima 90-53为供体亲本培育了一套陆地棉中棉所8号为背景的海岛棉染色体片段置换系(CSSLs), 本研究利用SSR标记对该置换系群体BC3F5进行基因型检测, 在3个不同环境下(河北保定、青县和新疆轮台)鉴定其纤维品质和产量相关性状并进行QTL定位。该置换系群体包含182个家系, 置换片段数在1~15个之间, 平均为6.6个; 导入片段长度在0.7~83.2 cM之间, 平均长度为16.8 cM; 置换片段总长度20 249.6 cM; 背景回复率在92.3%~99.6%之间, 平均为96.2%。共检测出59个相关的QTL, 其中与纤维品质性状相关的41个, 单个QTL的贡献率为1.27%~26.66%; 与产量性状相关的18个, 单个QTL的贡献率为2.03%~19.38%; 检测到14个稳定的QTL, 其中4个马克隆值和2个纤维伸长率相关的稳定QTL增效基因均来自高值亲本海岛棉Pima 90-53, 2个铃重相关的稳定QTL增效基因来自高值亲本陆地棉中棉所8号。研究结果为深入开展纤维品质和产量性状的QTL精细定位、QTL间互作和分子育种提供了理论依据。

关键词: 陆地棉, 海岛棉, 染色体片段置换系, 纤维品质, 产量, QTL定位

Abstract:

In the previous study, we developed a set of chromosome segment substitution lines (CSSLs) using G. hirsutum CCRI8 as the recipient parent and G. barbadense Pima 90-53 as the donor parent. In this study, we genotyped the BC3F5 generation of CSSLs with SSR markers, conducted QTL mapping for the fiber quality and yield traits and identified the stable QTLs in three different environments (Baoding, Qingxian, Luntai). The substituted segment number of the 182 CSSLs varied from one to fifteen, averaged 6.6. The length of introgressed segments ranged from 0.7 cM to 83.2 cM, and averaged 16.8 cM. The total length of the substituted fragment was 20 249.6 cM, background recoverage rate varied from 92.3% to 99.6%, and the average background recoverage rate was 96.2%. Fifty-nine QTLs related to fiber quality and yield traits were detected. Among them, 41 QTLs were related to fiber quality traits and each QTL explained 1.27% to 26.66% of the phenotypic variation. Eighteen QTLs for fiber yield-traits including boll weight and lint percentage were detected and each QTL explained 2.03% to 19.38% of the phenotypic variation. Fourteen stable QTLs were detected in multiple environments. Among them, four QTLs related to micronaire value and two QTLs related to fiber elongation both had enhancing alleles from G. barbadense Pima 90-53. Two boll weight QTLs had enhancing alleles from G. hirsutum CCRI8. The results provide a theoretical basis for QTL fine mapping, QTL interaction and molecular breeding for fiber quality and yield traits.

Key words: upland cotton, sea island cotton, CSSLs, fiber quality, yield, QTL mapping

图1

SSR引物扩增型 a: CCRI8基因型; b: Pima 90-53基因型; h: 杂合带型; -: 缺失带型。"

图2

导入片段长度分析示意图"

图3

染色体片段置换系基因型 左侧数字代表染色体编号(从下至上依次为A1、A2、……、A12、A13、D1、D2、……、D12、D13), 底部数字代表置换系编号(从左至右依次为CSSL1、CSSL2、……、CSSL181、CSSL182), 灰色、红色、蓝色分别代表受体亲本基因型、纯合供体亲本基因型和杂合基因型。"

图4

染色体片段置换系置换片段分析 A、B、C分别表示置换片段数量、置换片段长度、背景回复率。"

表1

染色体片段置换系置换片段覆盖情况"

染色体
Chromosome		 染色体总长度
Chromosome length (cM)		 覆盖长度
Coverage length (cM)		 缺失长度
Deletion length (cM)		 覆盖率
Coverage rate (%)
A1 82.7 82.7 0 100.0
A2 29.7 29.7 0 100.0
A3 167.7 167.7 0 100.0
A4 90.2 72.4 17.8 80.3
A5 255.8 255.8 0 100.0
A6 68.0 68.0 0 100.0
A7 111.1 105.7 5.4 95.1
A8 86.9 86.9 0 100.0
A9 151.8 151.8 0 100.0
A10 69.3 69.3 0 100.0
A11 113.4 113.4 0 100.0
A12 171.8 152.7 19.1 88.9
A13 56.8 56.8 0 100.0
D1 126.3 126.3 0 100.0
D2 155.8 142.3 13.5 91.3
D3 17.0 17.0 0 100.0
D4 51.2 51.2 0 100.0
D5 216.4 216.4 0 100.0
D6 83.9 83.9 0 100.0
D7 158.5 144.7 13.8 91.3
D8 94.2 94.2 0 100.0
D9 122.1 122.1 0 100.0
D10 95.7 95.7 0 100.0
D11 120.8 120.8 0 100.0
D12 149.1 131.4 17.7 88.1
D13 83.2 83.2 0 100.0

表2

亲本纤维品质和产量性状表现"

表3

染色体片段置换系纤维品质和产量性状统计分析"

性状
Trait		 地点
Location		 均值
Mean		 最小值
Min.		 最大值
Max.		 极差
Range		 标准差
SD		 变异系数
CV (%)		 偏度
Skewness		 峰度
Kurtosis
FL 保定Baoding 29.85 26.13 34.02 7.89 1.46 4.89 0.11 -0.01
轮台Luntai 29.89 26.18 34.14 7.96 1.29 4.32 0.35 0.60
青县Qingxian 28.84 25.13 32.00 6.87 1.24 4.30 -0.01 -0.02
FS 保定Baoding 33.47 23.70 40.10 16.40 2.50 7.47 -0.46 1.81
轮台Luntai 31.23 26.88 37.09 10.21 1.83 5.86 0.32 0.04
青县Qingxian 31.83 25.23 38.61 13.38 2.37 7.45 0.10 0.04
MIC 保定Baoding 4.92 3.30 5.97 2.67 0.48 9.76 -0.70 1.12
轮台Luntai 4.68 2.99 5.68 2.69 0.44 9.40 -0.85 1.63
青县Qingxian 5.15 3.78 6.23 2.45 0.44 8.54 -0.30 -0.01
FE 保定Baoding 6.07 4.32 8.16 3.84 0.72 11.86 0.23 -0.01
轮台Luntai 7.66 5.51 10.78 5.27 0.84 10.97 0.31 1.03
青县Qingxian 5.68 3.85 8.79 4.94 0.74 13.03 0.58 1.48
FU 保定Baoding 86.48 81.50 89.10 7.60 1.16 1.34 -0.50 1.20
轮台Luntai 85.91 83.57 88.40 4.83 0.93 1.08 -0.04 -0.14
青县Qingxian 84.90 81.30 87.40 6.10 1.13 1.33 -0.10 -0.42
LP 保定Baoding 37.54 27.85 44.75 16.90 0.03 0.08 -0.28 0.85
轮台Luntai 38.82 31.00 44.00 13.00 0.02 0.05 -0.49 0.45
青县Qingxian 38.68 30.07 47.32 17.25 0.03 0.08 -0.12 0.84
BW 保定Baoding 5.08 2.37 7.72 5.35 0.87 17.13 -0.31 0.82
轮台Luntai 5.69 3.45 7.42 3.97 0.74 13.01 -0.40 0.13
青县Qingxian 5.05 2.86 7.58 4.72 0.80 15.84 -0.26 0.85

表4

染色体片段置换系纤维品质、产量性状间的相关系数"

性状
Trait		 地点
Location		 FL FS MIC FE FU LP
FS 保定Baoding 0.279**
轮台Luntai 0.341**
青县Qingxian 0.486**
MIC 保定Baoding -0.095 0.213**
轮台Luntai -0.250** 0.038
青县Qingxian -0.011 0.151*
FE 保定Baoding -0.198** -0.128 -0.138
轮台Luntai -0.296** -0.198** -0.039
青县Qingxian -0.173* -0.104 -0.114
FU 保定Baoding 0.416** 0.565** 0.160* 0.565**
轮台Luntai 0.418** 0.476** 0.025 0.076
青县Qingxian 0.655** 0.577** 0.119 0.115
LP 保定Baoding -0.067 -0.027 0.083 -0.046 -0.055
轮台Luntai -0.051 -0.092 0.182* 0.051 0.056
青县Qingxian -0.016 -0.051 0.017 0.031 -0.020
BW 保定Baoding 0.064 0.013 0.148* 0.191** -0.034 0.046
轮台Luntai -0.106 -0.070 -0.074 -0.078 -0.057 0.214**
青县Qingxian -0.029 -0.106 -0.042 -0.091 -0.093 0.292**

图5

纤维品质和产量性状QTL定位在分子遗传图谱的位置"

表5

染色体片段置换系纤维品质和产量性状相关QTL"

性状
Trait		 QTL 染色体
Chr.		 标记区间
Interval		 位置
Position		 LOD 加性效应
Add		 贡献率
PVE (%)		 方向
Direction
FL qFL15-1-1 15 NAU5107b-NAU2094 1 3.44 1.04 8.56 Pima 90-53
qFL11-1-3 11 NAU4962-DPL0338 15 3.15 -1.14 8.73 CCRI8
FS qFS1-1-1 1 BNL3090-NAU5107c 60 4.67 0.75 3.56 Pima 90-53
qFS2-1-1 2 CER0061-NAU1053 18 3.26 -1.65 2.81 CCRI8
qFS5-1-1 5 NAU3450-NAU7067 30 3.53 0.07 2.03 Pima 90-53
qFS9-1-1 9 NAU2215-NAU2211 4 3.51 -1.03 4.57 CCRI8
qFS9-2-1 9 NAU2395-NAU1079 77 3.39 -0.09 4.09 CCRI8
qFS9-3-1 9 DPL0541-NAU6101 151 3.84 0.03 3.62 Pima 90-53
qFS10-1-1 10 NAU1236-NAU2082a 0 5.05 -4.71 2.42 CCRI8
qFS15-1-1 15 NAU5107b-NAU2094 1 5.07 1.18 4.02 Pima 90-53
qFS21-1-1 21 NAU3008-NAU7140b 55 5.13 -3.15 4.65 CCRI8
qFS14-1-2 14 NAU3820-NAU3474b 129 4.39 -0.69 7.09 CCRI8
qFS24-1-2 24 CER0091-NAU1133 91 4.13 -1.25 10.20 CCRI8
qFS5-1-3 5 NAU2140a-NAU2140b 150 3.47 3.16 7.66 Pima 90-53
MIC qMIC11-1-1 11 DPL0338-DPL0528a 18 4.36 0.44 7.72 Pima 90-53
qMIC15-1-1 15 NAU5107b-NAU2094 0 5.63 0.43 6.61 Pima 90-53
qMIC15-2-1 15 NAU7049-CIR009 106 5.00 0.30 5.74 Pima 90-53
qMIC22-1-1 22 STV191-NAU2302 38 3.46 0.79 4.68 Pima 90-53
qMIC23-1-1 23 NAU858a-BNL1317 44 5.98 -0.21 9.37 CCRI8
qMIC3-1-2 3 CER0028-DOW035 53 4.97 0.48 8.14 Pima 90-53
qMIC11-1-2 11 DPL0338-DPL0528a 26 3.05 0.42 8.50 Pima 90-53
qMIC23-1-2 23 NAU858a-BNL1317 45 6.07 -0.10 11.11 CCRI8
qMIC26-1-2 26 NAU1231-NAU2170 36 3.56 -0.06 7.12 CCRI8
qMIC3-1-3 3 CER0028-DOW035 48 3.35 0.33 6.66 Pima 90-53
qMIC11-1-3 11 NAU4962-DPL0338 8 5.93 0.57 12.45 Pima 90-53
qMIC13-1-3 13 NAU7130-NAU3468 17 3.37 -0.52 5.63 CCRI8
qMIC23-1-3 23 NAU858a-BNL1317 44 3.08 -0.12 8.95 CCRI8
FE qFE5-1-1 5 NAU7067-NAU3828 39 4.01 0.35 8.01 Pima 90-53
qFE14-1-1 14 NAU998a-NAU998b 29 3.23 0.62 7.55 Pima 90-53
qFE16-1-1 16 NAU3486b-NAU3486a 158 5.77 -0.79 9.28 CCRI8
qFE14-1-2 14 NAU998b-NAU7206 43 3.66 -0.73 7.86 CCRI8
qFE16-1-2 16 NAU3486b-NAU3486a 153 9.37 -1.33 26.66 CCRI8
qFE23-1-2 23 NAU6986a-NAU858a 24 4.65 0.63 10.19 Pima 90-53
qFE1-1-3 1 BNL3090-NAU5107c 50 5.00 0.64 10.86 Pima 90-53
qFE5-1-3 5 NAU7067-NAU3828 31 5.34 0.47 8.05 Pima 90-53
qFE16-1-3 16 NAU3486b-NAU3486a 129 8.03 -1.33 15.54 CCRI8
FU qFU24-1-1 24 NAU4099-NAU1369b 16 3.10 2.49 1.27 Pima 90-53
qFU23-1-1 23 BNL1317-NAU6764 52 3.32 -0.50 1.40 CCRI8
qFU3-1-2 3 CER0028-DOW035 54 3.31 0.90 5.60 Pima 90-53
qFU4-1-2 4 DOW046-DOW027 80 3.23 -0.36 7.35 CCRI8
qFU19-1-2 19 NAU3372-NAU1364 78 4.62 -0.94 7.80 CCRI8
LP qLP9-1-1 9 NAU2215-NAU2211 19 3.23 0.04 3.35 Pima 90-53
qLP1-1-2 1 TMF18-NAU2113 9 3.94 0.02 7.30 Pima 90-53
qLP11-1-2 11 DPL0338-DPL0528a 17 7.00 0.03 13.80 Pima 90-53
性状
Trait		 QTL 染色体
Chr.		 标记区间
Interval		 位置
Position		 LOD 加性效应
Add		 贡献率
PVE (%)		 方向
Direction
qLP17-1-2 17 DOW091-CER0076 17 4.00 0.02 5.86 Pima 90-53
qLP21-1-2 21 NAU3265-STV069 87 3.03 0.01 4.43 Pima 90-53
qLP11-1-3 11 NAU4962-DPL0338 15 6.74 0.04 10.07 Pima 90-53
qLP15-1-3 15 NAU7049-CIR009 111 3.22 0.02 9.72 Pima 90-53
BW qBW3-1-1 3 NAU972-NAU5035 16 3.10 -0.90 6.56 CCRI8
qBW3-2-1 3 NAU972-NAU5035 36 3.37 0.07 2.77 Pima 90-53
qBW3-3-1 3 NAU5035-CER0028 69 4.02 -1.08 8.80 CCRI8
qBW4-1-1 4 NAU1214-NAU7182 0 3.06 1.26 2.03 Pima 90-53
qBW6-1-1 6 NAU1385-NAU1110 183 4.30 0.46 5.37 Pima 90-53
qBW11-1-1 11 NAU4962-DPL0338 15 5.31 -0.95 3.91 CCRI8
qBW12-1-1 12 DOW045-NAU1368 48 5.53 -0.82 4.21 CCRI8
qBW15-1-1 15 NAU5107b-NAU2094 0 7.20 -0.82 5.41 CCRI8
qBW5-1-2 5 NAU786-DPL0241 220 5.43 -1.01 19.38 CCRI8
qBW11-1-3 11 NAU4962-DPL0338 15 7.55 -0.94 9.27 CCRI8
qBW12-1-3 12 NAU1368-NAU2251 49 5.65 -0.82 7.03 CCRI8

图6

遗传图谱与物理图谱共线性比较 A3 (C3)、A5 (C5)、A11 (C11)、D7 (C7)、D9 (C9)为遗传图谱, V3、V5、V11、V7、V9为物理图谱。"

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