作物学报 ›› 2019, Vol. 45 ›› Issue (5): 693-704.doi: 10.3724/SP.J.1006.2019.84120
Yi YUAN,Shuang ZHU,Ting-Ting FANG,Jin-Jin JIANG(),You-Ping WANG
摘要:
甘蓝型油菜是具有重要经济价值的多倍体物种, 是优质食用植物油和饲料蛋白质的重要来源之一。但是其驯化历史较短, 遗传背景狭窄, 且在整个生命周期中都对干旱胁迫敏感, 因此培育高产耐旱品种是甘蓝型油菜的重要育种目标之一。本文用15% PEG-6000模拟干旱胁迫, 对人工合成甘蓝型油菜不同世代(S1~S4)及其二倍体亲本进行不同时间的胁迫处理, 并结合表型观察, 以及叶片中丙二醛(MDA)、可溶性蛋白含量、过氧化物酶(POD)、超氧化物歧化酶(SOD)等生理指标的测定, 初步了解上述材料的抗旱性差异。结合表型观察和叶片中相对含水量分析, 发现人工合成甘蓝型油菜S1~S4及其亲本的抗旱性表现为甘蓝 > Bn-S3 > Bn-S4 > Bn-S1 > Bn-S2 > 白菜型油菜。干旱胁迫后Bn-S3、Bn-S4的POD及SOD活性较高, MDA含量较低, 表明Bn-S3和Bn-S4能更加有效地清除活性氧(ROS), 对过氧化损伤的防御能力更强。通过HPLC分析发现所有材料的甲基化水平在胁迫12 h时最高, 其中亲本白菜型油菜Br的甲基化水平最高, Bn-S1和Bn-S4介于两亲本之间, 而Bn-S2和Bn-S3低于两亲本。甲基化敏感多态性分析也显示人工合成甘蓝型油菜在干旱胁迫后, 甲基化和去甲基化水平均发生了明显的变化, 表明植物的甲基化变化可能有利于提高其抗旱能力。
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