作物学报 ›› 2022, Vol. 48 ›› Issue (12): 3120-3129.doi: 10.3724/SP.J.1006.2022.12077
郭均瑶1,2(), 刘斌美1,2, 杨惠杰1,2, 秦超琦2, 任艳2, 姜鸿瑞2, 陶亮之2, 叶亚峰2, 吴跃进1,2()
GUO Jun-Yao1,2(), LIU Bin-Mei1,2, YANG Hui-Jie1,2, QIN Chao-Qi2, REN Yan2, JIANG Hong-Rui2, TAO Liang-Zhi2, YE Ya-Feng2, WU Yue-Jin1,2()
摘要:
水稻(Oryza sativa L.)是重要的粮食作物之一, 其产量一直备受关注。水稻叶片是光合作用的重要场所, 叶脉是叶片中具有支撑和运输功能的组织。同时, 叶脉中存在的光合色素也能提供一定的光合作用。本研究采用重离子束诱变籼稻9311获得的叶脉黄化突变体yml进行遗传分析及基因定位研究。该突变体在抽穗期开花后5 d左右, 开始出现叶脉黄化表型, 并于抽穗后期黄化性状明显, 并持续至成熟期。在分蘖期, 突变体与野生型相比, 光合色素含量无显著差异。但在抽穗后期, 突变体叶片及叶脉中光合色素含量显著降低, 导致光合效率明显降低, 净光合速率仅为野生型的50.37%。成熟期突变体的株高、穗长、有效穗数、每穗实粒数、结实率、千粒重均显著低于野生型。通过遗传分析, 确定该突变性状由一对隐性基因控制。利用图位克隆技术, 将该基因定位于6号染色体。进一步通过简单重复序列(SSR)及插入缺失(InDel)标记, 将该基因位置缩小至InDel5与RM3431之间, 物理距离约为700 kb。本研究为后续突变体基因的克隆和功能分析提供了研究基础。
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