作物学报 ›› 2023, Vol. 49 ›› Issue (2): 561-569.doi: 10.3724/SP.J.1006.2023.22008
才晓溪(), 胡冰霜(), 沈阳, 王研, 陈悦, 孙明哲, 贾博为, 孙晓丽()
CAI Xiao-Xi(), HU Bing-Shuang(), SHEN Yang, WANG Yan, CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, SUN Xiao-Li()
摘要:
乙烯响应因子(ERF)是植物特有的一类转录因子, 在响应非生物胁迫中具有重要作用。本研究通过生物信息学发现, 野生大豆耐盐碱ERF转录因子GsERF6与水稻ERF同源蛋白的氨基酸序列相似性很高, 均包含1个高度保守的AP2结构域。为探究GsERF6基因在水稻耐盐碱应答中的作用, 通过遗传转化、PCR和半定量RT-PCR鉴定, 获得了2个纯合的GsERF6过表达转基因水稻株系。表型鉴定表明, 200 mmol L-1 NaHCO3处理下GsERF6转基因水稻的存活率、相对含水量、超氧化物歧化酶、过氧化物酶、过氧化氢酶活性、可溶性糖和脯氨酸含量均显著高于对照, 活性氧积累则反之。实时荧光定量PCR分析表明, 40 mmol L-1 NaHCO3处理6 h后渗透调节基因OsP5CS2和OsLEA14在GsERF6转基因水稻中的表达量显著高于对照。本研究表明, 水稻中GsERF6的过表达可通过提高ROS清除水平、渗透调节能力及胁迫应答基因的表达来提高其耐盐碱性。
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