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作物学报 ›› 2024, Vol. 50 ›› Issue (11): 2742-2753.doi: 10.3724/SP.J.1006.2024.41007
所属专题： 小麦：遗传育种·种质资源·分子遗传学
李云香1(
), 张思甜1, 侯万伟2, 张小娟1,*()
LI Yun-Xiang1(), ZHANG Si-Tian1, HOU Wan-Wei2, ZHANG Xiao-Juan1,*()
摘要：
以引进国际干旱地区农业研究中心(ICARDA)的159份小麦为研究对象, 在苗期用20% PEG-6000模拟干旱条件进行处理, 以正常营养液作为对照, 分析干旱环境对7个苗期相关生理性状(丙二醛含量、超氧化物歧化酶活性、相对电导率、过氧化物酶活性、可溶性糖含量、脯氨酸含量和叶绿素含量)的影响, 进行相关性分析, 并结合55K SNP芯片对159份小麦的苗期抗旱相关生理性状进行关联分析。研究结果表明, 干旱处理后, 其体内脯氨酸含量和可溶性糖含量均呈现上升趋势, 其余性状则并未表现出一致的上升或下降。相关性分析表明, 在正常处理条件下, 丙二醛含量与过氧化物酶活性呈显著正相关, 和脯氨酸含量呈极显著负相关; 过氧化物酶活性和可溶性糖含量呈极显著负相关。在干旱胁迫处理条件下, 可溶性糖含量与丙二醛含量呈极显著正相关, 与相对电导率呈显著正相关; 叶绿素含量与可溶性糖含量呈极显著正相关, 与脯氨酸含量呈显著负相关。关联分析结果显示, 利用24,151个SNP标记位点结合苗期相关生理性状在P≤0.001水平下共定位到311个抗旱相关标记, 分布于小麦的21条染色体上, 贡献率为7.13%~21.11%。2种处理下检测到8个稳定位点, 分别位于1B、2B和6A染色体上, 贡献率为7.85%~14.58%。检测到1个多效应位点, 同时关联了超氧化物歧化酶活性和可溶性糖含量2个性状, 位于3D染色体上, 贡献率为7.95%~8.69%。检测到4个显著位点与相对电导率、过氧化物酶活性、叶绿素含量的抗旱系数和干旱处理下的相对电导率、过氧化物酶活性、脯氨酸含量和超氧化物歧化酶活性等性状相关联, 分别位于2D、3A和4A染色体上, 贡献率为7.61%~14.74%。
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