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作物学报 ›› 2025, Vol. 51 ›› Issue (6): 1480-1488.doi: 10.3724/SP.J.1006.2025.42049
凤舞剑1,*(
), 冼晓青2, 张新钵1, 曹丹1, 强承魁1
FENG Wu-Jian1,*(), XIAN Xiao-Qing2, ZHANG Xin-Bo1, CAO Dan1, QIANG Cheng-Kui1
摘要：
效应蛋白是植物病原菌克服植物免疫的重要武器, 病原菌在侵染初期与植物的斗争中会分泌不同类型的效应子。为了鉴定病原菌关键的经典效应蛋白和与植物互作的靶基因, 拟通过生物信息学和结构生物学建立经典效应蛋白和靶标蛋白的鉴定方案。以稻瘟病菌和水稻为对象, 运用SignalP、TMHMM、PredGPI、PSORT和EffectorP在稻瘟病菌中共鉴定到535个效应蛋白, 主要分为5类。利用稻瘟病菌-水稻互作转录组共鉴定到282个关键效应蛋白, 并构建了稻瘟病菌-水稻的早期效应蛋白-植物互作的共表达网络。利用AlphaFold3预测发现水稻Os06t0633800和Os03t0114400蛋白可能分别为稻瘟病菌MGG_08817和MGG_03865的潜在靶标。利用荧光素酶互作验证发现MGG_08817与Os06t0633800、MGG_03865与Os03t0114400在烟草中存在互作。病原菌效应蛋白和靶标蛋白的快速筛选和鉴定对于植物病害的防治具有重要意义, 该研究成果将有助于鉴定和挖掘重要的病原菌效应蛋白和植物靶基因, 为植物病原菌互作的深入研究提供理论依据, 为植物病害的绿色防控奠定基础。
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