基于BSA-seq技术的块茎芽眼深度QTL定位分析

doi:10.3724/SP.J.1006.2025.44201

作物学报 ›› 2025, Vol. 51 ›› Issue (7): 1725-1735.doi: 10.3724/SP.J.1006.2025.44201

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

基于BSA-seq技术的块茎芽眼深度QTL定位分析

邵顺伟^1,2,陈卓¹,兰振东^1,2,蔡兴奎²,邹华芬¹,李晨曦²,唐景华¹,朱熙^1,张彧¹,董建科²,金辉^1,*,宋波涛^2,*

1中国热带农业科学院南亚热带作物研究所 / 农业农村部热带果树生物学重点实验室 / 海南省热带园艺产品采后生理与保鲜重点实验室, 广东湛江524091; 2果蔬园艺作物种质创新与利用全国重点实验室 / 农村农业部马铃薯生物学与生物技术重点实验室 / 华中农业大学, 湖北武汉430070

收稿日期:2024-12-03 修回日期:2025-04-27 接受日期:2025-04-27 出版日期:2025-07-12 网络出版日期:2025-05-07
基金资助:
本研究由广东省重点领域研发计划项目(2022B0202060001), 中央级公益性科研院所基本科研业务费专项(1630062024011), 海南省自然科学基金青年基金项目(323QN296, 323QN328)和国家自然科学基金地区基金项目(32360459)资助。

QTL mapping of tuber eye depth based on BSA-seq technique

SHAO Shun-Wei^1,2,CHEN Zhuo¹,LAN Zhen-Dong^1,2,CAI Xing-Kui²,ZOU Hua-Fen¹,LI Chen-Xi²,TANG Jing-Hua¹,ZHU Xi¹,ZHANG Yu¹,DONG Jian-Ke²,JIN Hui^1,*,SONG Bo-Tao^2,*

1Institute of South Subtropical Crop Research, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs / Key Laboratory for Post-harvest Physiology and Preservation of Tropical Horticultural Products of Hainan, Zhanjiang 524091, Guangdong, China; 2 National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops / Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs / Huazhong Agricultural University, Wuhan 430070, Hubei, China

Received:2024-12-03 Revised:2025-04-27 Accepted:2025-04-27 Published:2025-07-12 Published online:2025-05-07
Supported by:
This study was supported by the Guangdong Provincial Key Areas Research and Development Program (2022B0202060001), the Special Fund for Basic Scientific Research Operating Expenses of Central-level Public Welfare Research Institutes (1630062024011), the Hainan Provincial Natural Science Foundation Youth Fund (323QN296, 323QN328), and the Regional Fund of the National Natural Science Foundation of China (32360459).

摘要/Abstract

摘要：

芽眼深度是马铃薯块茎的重要性状之一，对马铃薯的外观品质及加工适宜性具有重要影响。为挖掘控制马铃薯芽眼深度的数量性状位点，本研究以四倍体深芽眼品种“华薯12”为母本、浅芽眼高代品系“天2002-4-5”为父本，杂交获得F₁代分离群体，共255份无性系。基于连续2年田间表型观测数据，分别选取20个深芽眼和20个浅芽眼个体构建混合池，利用BSA-seq技术进行芽眼深度相关性状的QTL定位，并结合传统QTL定位方法，采用完备区间作图法构建遗传连锁图谱，成功定位到2个与芽眼深度相关的QTL位点。2年表型相关性分析结果表明，马铃薯块茎芽眼深度主要受遗传因素的控制。10号染色体上qEyd10.1位点的LOD值为4.96，表型贡献率为14.49%；3号染色体上qEyd3.1位点的LOD值为3.29，表型贡献率为10.18%。其中，qEyd3.1与此前报道的芽眼深度调控位点一致，而qEyd10.1为新发现的QTL。2个位点的加性效应均为负值，表明降低芽眼深度的等位基因来源于浅芽眼亲本“天2002-4-5”。通过对定位区间内候选基因注释结合芽眼深浅材料基因结构变异分析，推测Soltu.DM.10G029390.1、Soltu.DM.03G036540.1、Soltu.DM.03G036140.1和Soltu.DM.03G036580.1可能为与芽眼深度相关的候选基因。本研究通过BSA-seq与传统QTL定位相结合的方式，快速完成了同源四倍体马铃薯中芽眼深度这一数量性状的调控位点定位工作，并初步确定了4个候选基因，为进一步完成芽眼深度调控基因的克隆及其遗传机制解析奠定了重要基础，也为培育具有浅芽眼块茎的四倍体马铃薯新品种提供了参考。

关键词: 马铃薯, 芽眼深度, QTL定位, BSA-seq, 遗传连锁图谱

Abstract: Eye depth is an important trait of potato tubers, significantly affecting both their appearance and processing quality. To identify quantitative trait loci (QTLs) associated with eye depth, a cross was made between the tetraploid deep-eyed variety “Hua Shu 12” (female) and the shallow-eyed advanced line “Tian 2002-4-5” (male), generating 255 clonal F1 progeny. Based on field phenotypic data collected over two consecutive years, 20 deep-eyed and 20 shallow-eyed individuals were selected to construct bulks for QTL mapping. The BSA-seq approach was employed to detect QTLs related to eye depth, and combined with traditional QTL mapping methods, a complete interval mapping analysis was conducted to construct a genetic linkage map. Two QTLs associated with eye depth were successfully identified. Phenotypic correlation analysis across both years suggested that tuber eye depth is primarily controlled by genetic factors. The LOD score for locus qEyd10.1 on chromosome 10 was 4.96, with a phenotypic variance explained (PVE) of 14.49%, while qEyd3.1 on chromosome 3 had a LOD score of 3.29 and a PVE of 10.18%. Notably, qEyd3.1 corresponds to a previously reported eye depth locus, whereas qEyd10.1 represents a novel QTL. Both loci exhibited negative additive effects, indicating that the allele responsible for reduced eye depth was inherited from the shallow-eyed parent “Tian 2002-4-5”. Through candidate gene annotation within the mapped intervals and analysis of gene structural variation between deep- and shallow-eyed materials, four candidate genes: Soltu.DM.10G029390.1, Soltu.DM.03G036540.1, Soltu.DM.03G036140.1, and Soltu.DM.03G036580.1 were preliminarily identified as potentially associated with eye depth. This study, by integrating BSA-seq with conventional QTL mapping in autotetraploid potato, provides a preliminary identification of candidate genes regulating tuber eye depth. These findings lay a foundation for future gene cloning and genetic mechanism studies, and offer a valuable reference for breeding new tetraploid potato varieties with shallower eyes.

Key words: potato, eye depth, QTL mapping, bulked segregant analysis sequencing, genetic linkage map

邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735.

SHAO Shun-Wei, CHEN Zhuo, LAN Zhen-Dong, CAI Xing-Kui, ZOU Hua-Fen, LI Chen-Xi, TANG Jing-Hua, ZHU Xi, ZHANG Yu, DONG Jian-Ke, JIN Hui, SONG Bo-Tao. QTL mapping of tuber eye depth based on BSA-seq technique[J]. Acta Agronomica Sinica, 2025, 51(7): 1725-1735.

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基于BSA-seq技术的块茎芽眼深度QTL定位分析

QTL mapping of tuber eye depth based on BSA-seq technique

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