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作物学报 ›› 2025, Vol. 51 ›› Issue (2): 301-311.doi: 10.3724/SP.J.1006.2025.44098

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘薯激酶基因IbHT1的克隆及抗旱性功能鉴定

王语新(), 陈天羽, 翟红, 张欢, 高少培, 何绍贞, 赵宁, 刘庆昌()   

  1. 农业农村部甘薯生物学与生物技术重点实验室 / 中国农业大学农学院, 北京 100193
  • 收稿日期:2024-06-17 接受日期:2024-09-18 出版日期:2025-02-12 网络出版日期:2024-10-11
  • 通讯作者: 刘庆昌, E-mail: liuqc@cau.edu.cn
  • 作者简介:E-mail: 978067241@qq.com
  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-10)

Cloning and characterization of drought tolerance function of kinase gene IbHT1 in sweetpotato

WANG Yu-Xin(), CHEN Tian-Yu, ZHAI Hong, ZHANG Huan, GAO Shao-Pei, HE Shao-Zhen, ZHAO Ning, LIU Qing-Chang()   

  1. Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs / College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
  • Received:2024-06-17 Accepted:2024-09-18 Published:2025-02-12 Published online:2024-10-11
  • Contact: E-mail: liuqc@cau.edu.cn
  • Supported by:
    China Agriculture Research System of MOF and MARA(CARS-10)

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摘要：

HT1 (HIGH LEAF TEMPERATURE 1)属于蛋白激酶, 在模式植物拟南芥中主要参与气孔运动, 但其在甘薯(Ipomoea batatas (L.) Lam.)中的作用未见相关报道。本研究从甘薯品系徐薯55-2中克隆得到IbHT1基因, 其CDS全长1140 bp, 编码379个氨基酸。IbHT1蛋白具有一个保守的STKc_MAP3K_Like蛋白激酶结构域, 预测分子量大小43.07 kD, 等电点8.83。其基因组全长2796 bp, 含有3个外显子和2个内含子。IbHT1蛋白定位于细胞膜上, 其蛋白全长无转录激活活性。IbHT1基因受20% PEG-6000诱导下调表达。过表达IbHT1基因减弱了甘薯植株的抗旱性, 而RNA干扰该基因显著增强了甘薯植株的抗旱性。通过酵母筛库筛选到与IbHT1蛋白互作的10个蛋白, 由此推测, IbHT1蛋白激酶可能通过与这些蛋白相互作用从而共同参与甘薯抗旱性的调控。

关键词: 甘薯, IbHT1, RNA干扰, 抗旱性, 互作蛋白

Abstract:

HT1 (HIGH LEAF TEMPERATURE 1) is a protein kinase known for its role in regulating stomatal movement in Arabidopsis. However, its function in sweetpotato has not been reported. In this study, the IbHT1 gene was cloned from the sweetpotato line Xushu 55-2. The full-length CDS of IbHT1 is 1140 bp, encoding a 379-amino acid protein that contains a conserved STKc_MAP3K_Like domain, with a predicted molecular weight of 43.07 kD and an isoelectric point (pI) of 8.83. The genomic sequence of IbHT1 spans 2796 bp, comprising 3 exons and 2 introns. Subcellular localization analysis revealed that the IbHT1 protein is localized to the cell membrane, and yeast assays confirmed it lacks transactivation activity. Expression of IbHT1 was down-regulated in response to 20% PEG-6000 treatment. Overexpression of IbHT1 significantly reduced drought tolerance in sweetpotato, while RNA interference (RNAi) of IbHT1 markedly enhanced drought tolerance. Additionally, 10 proteins interacting with IbHT1 were identified through yeast library screening. These findings suggest that IbHT1 may regulate drought tolerance in sweetpotato by interacting with other proteins.

Key words: sweetpotato, IbHT1, RNA interference, drought tolerance, interaction protein

表1

本研究所用引物序列"

引物名称
Primer name		 序列
Primer sequence (5°-3°)
IbHT1基因CDS及基因组引物Primers for CDS and genomic DNA
IbHT1-ORF-F ATGGGTGGCTATTGCTTCAA
IbHT1-ORF-R TCAGACATGTACAGGTATGGATGAA
实时荧光定量引物Primers for RT-qPCR
Actin-F AGCAGCATGAAGATTAAGGTTGTAGCACT
Actin-R GGAAAATTAGAAGCACTTCCTGTGAAC
IbHT1-F GGAGCTCACCACTTCTTTGC
IbHT1-R CGCGGAGACTATGTCTGTGA
亚细胞定位和过表达载体引物Primers for subcellular localization and overexpression vectors
IbHT1-OE-F ACGGGGGACGAGCTCGGTACCATGGGTGGCTATTGCTTCAA
IbHT1-OE-R CATGTCGACTCTAGAGGATCCGACATGTACAGGTATGGATGAACTG
转录激活活性分析引物Primers for transactivation activity assay
pGBKT7-IbHT1-F ATGGCCATGGAGGCCGAATTCATGGGTGGCTATTGCTTCAA
pGBKT7-IbHT1-R CCGCTGCAGGTCGACGGATCCTCAGACATGTACAGGTATGGATGAA
蛋白纯化分析引物Primers for protein purification
pET-28a-IbHT1-F CAGCAAATGGGTCGCGGATCCATGGGTGGCTATTGCTTCAA
pET-28a-IbHT1-R TTGTCGACGGAGCTCGAATTCGACATGTACAGGTATGGATGAA
RNA干扰载体引物Primers for RNAi vectors
IbHT1-RNAi-F1 TTTGGAGAGGACACGCTCGAGATGGGTGGCTATTGCTTCAAT
IbHT1-RNAi-R1 AGAAATTCTTACACATTTAAATGCCTCCCACGTTTCGACAT
IbHT1-RNAi-F2 AATTTGCAGGTATTTGGATCCGCCTCCCACGTTTCGACAT
IbHT1-RNAi-R2 GGTCTTAATTAACTCTCTAGAATGGGTGGCTATTGCTTCAAT
转基因植株鉴定引物Primers for identifying transgenic plants
35S-IbHT1-F GACGCACAATCCCACTATCC
IbHT1-ORF-R TCAGACATGTACAGGTATGGATGAA
int-F CAACCACAAAAGTATCTATGAGCCT
int-R TTCACATGTCAGAAACATTCTGATG

图1

IbHT1基因的克隆及序列分析 A: IbHT1基因扩增产物; B: HT1蛋白序列的多重比较; C: IbHT1蛋白结构示意图; D: IbHT1蛋白进化树分析; E: IbHT1与AtHT1的基因组结构比较。"

图2

IbHT1基因在徐薯55-2中的表达分析 A: 在20% PEG-6000处理下IbHT1在徐薯55-2试管苗中的表达分析; B: IbHT1在徐薯55-2试管苗的根(R)、茎(S)、叶柄(P)和叶(L)中的表达分析; C: IbHT1在徐薯55-2大田植株的块根(SR)、梗根(PR)、须根(FR)、茎(S)和叶(L)中的表达分析。**表示用Student’s t测验在P < 0.01水平差异显著, 不同小写字母表示用ANOVA post-hoc Tukey测验在P < 0.05水平差异显著。"

图3

IbHT1蛋白在水稻原生质体中亚细胞定位 PIP2A-mCherry为细胞膜定位marker, 标尺为20 μm。"

图4

在酵母细胞中IbHT1蛋白的转录激活活性分析"

图5

His-IbHT1纯化蛋白"

图6

转IbHT1基因甘薯植株的获得 A: 在含有2.0 mg L-1 2,4-D的MS液体培养基中迅速生长的栗子香胚性悬浮细胞系; B: 在含有2.0 mg L-1 2,4-D、100 mg L-1头孢霉素和11.0 mg L-1潮霉素(Hyg)的MS固体培养基上筛选后形成的Hyg抗性胚性愈伤组织(亮黄色); C: Hyg抗性愈伤组织在含有1.0 mg L-1 ABA和100 mg L-1 头孢霉素的MS固体培养基上再生植株; D: 在MS基本培养基上获得的完整再生植株; E, F: IbHT1转基因植株的PCR鉴定。M: DL 2000 marker; W: 水(阴性对照); WT: 栗子香(阴性对照); P: 质粒(阳性对照); OH-1~OH-11: 过表达植株; RH-1~RH-4: RNA干扰植株; G, H: 在正常条件下转基因植株中IbHT1基因的表达分析。**表示用Student’s t测验在P < 0.01水平差异显著。"

图7

转IbHT1基因甘薯植株的抗旱性鉴定 A, B: 在正常MS培养基上植株的生长情况与鲜重(对照); C, D: 在含有20% PEG-6000的MS培养基上植株的生长情况与鲜重。标尺为10 cm。*和**分别表示用Student’s t测验在P < 0.05和P < 0.01水平差异显著。WT: 野生型; OH-10和OH-11: IbHT1基因过表达株系; RH-3和RH-4: IbHT1基因RNA干扰株系。"

表2

酵母双杂交筛选IbHT1拟互作蛋白"

蛋白名称 Protein name 功能描述 Functional description
IbHT1互作蛋白-1
IbHT1 interaction protein-1		 铁硫簇共伴侣蛋白HscB同源物
Iron-sulfur cluster co-chaperone protein HscB homolog
IbHT1互作蛋白-2
IbHT1 interaction protein-2		 尿苷激酶样蛋白类似物
Uridine kinase-like protein 1, chloroplastic
IbHT1互作蛋白-3
IbHT1 interaction protein-3		 转录因子VOZ1类似物
Transcription factor VOZ1-like
IbHT1互作蛋白-4
IbHT1 interaction protein-4		 转录因子MYB1R1类似物
Transcription factor MYB1R1-like
IbHT1互作蛋白-5
IbHT1 interaction protein-5		 ACR11蛋白类似物
ACR11-like
IbHT1互作蛋白-6
IbHT1 interaction protein-6		 ATP合成酶
ATP synthase gamma chain, chloroplastic
IbHT1互作蛋白-7
IbHT1 interaction protein-7		 DUF3444蛋白
DUF3444 protein
IbHT1互作蛋白-8
IbHT1 interaction protein-8		 30S核糖体蛋白S6 α类似物
30S ribosomal protein S6 alpha, chloroplastic-like
IbHT1互作蛋白-9
IbHT1 interaction protein-9		 鸟氨酸转氨酶
Ornithine aminotransferase, mitochondrial
IbHT1互作蛋白-10
IbHT1 interaction protein-10		 细胞色素P450
Cytochrome P450
[1] 赵杨, 杨永青, 丁杨林, 张蘅, 谢彦杰, 赵春钊, 刘林川, 王鹏程. 植物非生物逆境学科发展综述. 植物生理学报, 2024, 60: 248-270.
Zhao Y, Yang Y Q, Ding Y L, Zhang H, Xie Y J, Zhao C Z, Liu L C, Wang P C. Plant abiotic stress biology: a decade update. Plant Physiol J, 2024, 60: 248-270 (in Chinese with English abstract).
[2] Zhu J K. Abiotic stress signaling and responses in plants. Cell, 2016, 167: 313-324.
[3] 任洪雷, 朱筱, 张丰屹, 张必弦, 王家军, 王金生, 吴俊江, 王广金, 邱丽娟. 干旱胁迫的影响及抗旱性研究进展. 分子植物育种, 网络首发[2024-01-22], https://link.cnki.net/urlid/46.1068.S.20240119.
Ren H L, Zhu X, Zhang F Y, Zhang B X, Wang J J, Wang J S, Wu J J, Wang G J, Qiu L J. Effect of drought stress and research progress of drought resistance. Mol Plant Breed, Published online [2024-01-22], https://link.cnki.net/urlid/46.1068.S.20240119. (in Chinese with English abstract).
[4] 朱婷婷, 王彦霞, 裴丽丽, 谢传磊, 陈明, 陈隽, 周永斌, 马有志, 徐兆师. 植物蛋白激酶与作物非生物胁迫抗性的研究. 植物遗传资源学报, 2017, 18: 763-770.
doi: 10.13430/j.cnki.jpgr.2017.04.020
Zhu T T, Wang Y X, Pei L L, Xie C L, Chen M, Chen J, Zhou Y B, Ma Y Z, Xu Z S. Research progress of plant protein kinase and abiotic stress resistance. J Plant Genet Resour, 2017, 18: 763-770 (in Chinese with English abstract).
doi: 10.13430/j.cnki.jpgr.2017.04.020
[5] 张鑫苗, 伍国强, 魏明. MAPK在植物响应逆境胁迫中的作用. 草业学报, 2024, 33(1): 182-197.
doi: 10.11686/cyxb2023090
Zhang X M, Wu G Q, Wei M. The role of MAPK in plant response to abiotic stress. Acta Pratac Sin, 2024, 33(1): 182-197 (in Chinese with English abstract).
[6] Chen X X, Ding Y L, Yang Y Q, Song C P, Wang B S, Yang S H, Guo Y, Gong Z Z. Protein kinases in plant responses to drought, salt, and cold stress. J Integr Plant Biol, 2021, 63: 53-78.
doi: 10.1111/jipb.13061
[7] Chen J, Wang L H, Yuan M. Update on the roles of rice MAPK cascades. Int J Mol Sci, 2021, 22: 1679.
[8] Li Y Y, Cai H X, Liu P, Wang C Y, Gao H Y, Wu C G, Yan K, Zhang S Z, Huang J G, Zheng C C. Arabidopsis MAPKKK18 positively regulates drought stress resistance via downstream MAPKK3. Biochem Biophys Res Commun, 2017, 484: 292-297.
[9] Ning J, Li X H, Hicks L M, Xiong L Z. A Raf-Like MAPKKK gene DSM1 mediates drought resistance through reactive oxygen species scavenging in rice. Plant Physiol, 2010, 152: 876-890.
[10] Ma H G, Chen J, Zhang Z Z, Ma L, Yang Z Y, Zhang Q L, Li X H, Xiao J H, Wang S P. MAPK kinase 10.2 promotes disease resistance and drought tolerance by activating different MAPKs in rice. Plant J, 2017, 92: 557-570.
[11] Zhao L L, Yan J W, Xiang Y, Sun Y, Zhang A Y. ZmWRKY104 transcription factor phosphorylated by ZmMPK6 functioning in ABA-induced antioxidant defense and enhance drought tolerance in maize. Biology, 2021, 10: 893.
[12] Li F J, Li M Y, Wang P, Cox K L Jr, Duan L S, Dever J K, Shan L B, Li Z H, He P. Regulation of cotton (Gossypium hirsutum) drought responses by mitogen-activated protein (MAP) kinase cascade-mediated phosphorylation of GhWRKY59. New Phytol 2017, 215: 1462-1475.
[13] Jeong S, Lim C W, Lee S C. The pepper MAP Kinase CaAIMK1 positively regulates ABA and drought stress responses. Front Plant Sci, 2020, 11: 720.
doi: 10.3389/fpls.2020.00720 pmid: 32528517
[14] Wang J Y, Chitsaz F, Derbyshire M K, Gonzales N R, Gwadz M, Lu S N, Marchler G H, Song J S, Thanki N, Yamashita R A, Yang M Z, Zhang D C, Zheng C J, Lanczycki C J, Marchler-Bauer A. The conserved domain database in 2023. Nucleic Acids Res, 2023, 51: D384-D388.
[15] Hashimoto M, Negi J, Young J, Israelsson M, Schroeder J I, Iba K. Arabidopsis HT1 kinase controls stomatal movements in response to CO2. Nat Cell Biol, 2006, 8: 391-397.
pmid: 16518390
[16] Matrosova A, Bogireddi H, Mateo-Peñas A, Hashimoto-Sugimoto M, Iba K, Schroeder J I, Israelsson-Nordström M. The HT1 protein kinase is essential for red light-induced stomatal opening and genetically interacts with OST1 in red light and CO2-induced stomatal movement responses. New Phytol, 2015, 208: 1126-1137.
[17] Horak H, Sierla M, Toldsepp K, Wang C, Wang Y S, Nuhkat M, Valk E, Pechter P, Merilo E, Salojarvi J, Overmyer K, Loog M, Brosche M, Schroeder J I, Kangasjarvi J, Kollist H. A dominant mutation in the HT1 kinase uncovers roles of MAP kinases and GHR1 in CO2-induced stomatal closure. Plant Cell, 2016, 28: 2493-2509.
[18] Gahlowt P, Tripathi D K, Singh S, Gupta R, Singh V P. Does MPK4/12-HT1 function as a CO2/bicarbonate sensor to regulate the stomatal conductance under high CO levels. Plant Cell Rep, 2023, 42: 2043-2045.
doi: 10.1007/s00299-023-03077-8 pmid: 37815540
[19] 后猛, 李臣, 张允刚, 闫会, 王欣, 唐维, 宋炜涵, 高闰飞, 李强. 优质高产淀粉型甘薯徐薯37选育及性状鉴定. 江苏师范大学学报(自然科学版), 2023, 41(3): 45-47.
Hou M, Li C, Zhang Y G, Yan H, Wang X, Tang W, Song W H, Gao R F, Li Q. Breeding and character identification of a sweetpotato variety Xushu 37 for starch use with high yield and quality. J Jiangsu Norm Univ (Nat Sci Edn), 2023, 41(3): 45-47 (in Chinese with English abstract).
[20] Wang Z, Li X, Gao X R, Dai Z R, Peng K, Jia L C, Wu Y K, Liu Q C, Zhai H, Gao S P, Zhao N, He S Z, Zhang H. IbMYB73 targets abscisic acid-responsive IbGER5 to regulate root growth and stress tolerance in sweet potato. Plant Physiol, 2024, 194: 787-804.
[21] Yan M X, Li M, Wang Y Z, Wang X Y, Moeinzadeh M H, Quispe-Huamanquispe D G, Fan W J, Fang Y J, Wang Y Q, Nie H Z, Wang Z Y, Tanaka A, Heider B, Kreuze J F, Gheysen G, Wang H X, Vingron M, Bock R, Yang J. Haplotype-based phylogenetic analysis and population genomics uncover the origin and domestication of sweetpotato. Mol Plant, 2024, 17: 277-296.
[22] 吴胜男, 孙凯, 张海, 刘峰, 王凤. 甘薯分子标记辅助育种研究进展. 黑龙江农业科学, 2022, (9): 111-115.
Wu S N, Sun K, Zhang H, Liu F, Wang F. Research progress of sweet potato molecular marker-assisted breeding. Heilongjiang Agric Sci, 2022, (9): 111-115 (in Chinese with English abstract).
[23] Jin R, Kim B H, Ji C Y, Kim H S, Li H M, Ma D F, Kwak S S. Overexpressing IbCBF3 increases low temperature and drought stress tolerance in transgenic sweetpotato. Plant Physiol Biochem, 2017, 118: 45-54.
[24] Zhou Y Y, Zhai H, Xing S H, Wei Z H, He S Z, Zhang H, Gao S P, Zhao N, Liu Q C. A novel small open reading frame gene. IbEGF, enhances drought tolerance in transgenic sweet potato. Front Plant Sci, 2022, 13: 965069.
[25] Ren Z T, He S Z, Zhou Y Y, Zhao N, Jiang T, Zhai H, Liu Q C. A sucrose non-fermenting-1-related protein kinase-1 gene, IbSnRK1, confers salt, drought and cold tolerance in sweet potato. Crop J, 2020, 8: 905-917.
[26] Wang Y X, Zhang H, Gao S P, Zhai H, He S Z, Zhao N, Liu Q C. An ABA-inducible gene IbTSJT1positively regulates drought tolerance in transgenic sweetpotato. J Integr Agric, Published online [2023-10-18], https://doi.org/10.1016/j.jia.2023.10.015
[27] Zhang H, Gao X R, Zhi Y H, Li X, Zhang Q, Niu J B, Wang J, Zhai H, Zhao N, Li J G, Liu Q C, He S Z. A non-tandem CCCH-type zinc-finger protein, IbC3H18, functions as a nuclear transcriptional activator and enhances abiotic stress tolerance in sweet potato. New Phytol, 2019, 223: 1918-1936.
doi: 10.1111/nph.15925 pmid: 31091337
[28] Zhang H, Wang Z, Li X, Gao X R, Dai Z R, Cui Y F, Zhi Y H, Liu Q C, Zhai H, Gao S P, Zhao N, He S Z. The IbBBX24- IbTOE3-IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato. New Phytol, 2022, 233: 1133-1152.
[29] Xue L Y, Wei Z H, Zhai H, Xing S H, Wang Y X, He S Z, Gao S P, Zhao N, Zhang H, Liu Q C. The IbPYL8-IbbHLH66- IbbHLH118 complex mediates the abscisic acid-dependent drought response in sweet potato. New Phytol, 2022, 236: 2151-2171.
[30] 周桦楠, 于涛, 刘冠求, 潘家荃, 万博, 刘振雷. 甘薯MAPK基因家族的鉴定及生物信息学分析. 沈阳农业大学学报, 2021, 52: 513-520.
Zhou H N, Yu T, Liu G Q, Pan J Q, Wan B, Liu Z L. Genome-wide identification and bioinformatic analysis of mitogen activated protein kinase gene family in sweet potato. J Shenyang Agric Univ, 2021, 52: 513-520 (in Chinese with English abstract).
[31] 靳容, 刘明, 赵鹏, 张强强, 张爱君, 唐忠厚. 甘薯丝裂原活化蛋白激酶MPK6对低温胁迫的响应. 中国农业科学, 2021, 54: 4265-4273.
doi: 10.3864/j.issn.0578-1752.2021.20.002
Jin R, Liu M, Zhao P, Zhang Q Q, Zhang A J, Tang Z H. IbMKP6, A mitogen-activated protein kinase, confers low temperature tolerance in sweetpotato. Sci Agric Sin, 2021, 54: 4265-4273 (in Chinese with English abstract).
[32] 靖小菁, 杨新笋, 靳晓杰, 刘意, 雷剑, 王连军, 柴沙沙, 张文英, 焦春海. 甘薯蔓割病(Fusarium oxysporum f. sp. batatas)相关基因IbMAPKK9的克隆与特性分析. 作物学报, 2023, 49: 3289-3301.
doi: 10.3724/SP.J.1006.2023.24284
Jing X J, Yang X S, Jin X J, Liu Y, Lei J, Wang L J, Chai S S, Zhang W Y, Jiao C H. Cloning and characterization of IbMAPKK9 gene associated with Fusarium oxysporum f. sp. batatas in sweet potato. Acta Agron Sin, 2023, 49: 3289-3301 (in Chinese with English abstract).
[33] Zhu H, Zhou Y Y, Zhai H, He S Z, Zhao N, Liu Q C. Transcriptome profiling reveals insights into the molecular mechanism of drought tolerance in sweetpotato. J Integr Agric, 2019, 18: 9-23.
doi: 10.1016/S2095-3119(18)61934-3
[34] Xing S H, Zhu H, Zhou Y Y, Xue L Y, Wei Z H, Wang Y X, He S Z, Zhang H, Gao S P, Zhao N, Zhai H, Liu Q C. A cytochrome P450 superfamily gene, IbCYP82D47, increases carotenoid contents in transgenic sweet potato. Plant Sci, 2022, 318: 111233.
[35] Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol, 2018, 35: 1547-1549.
doi: 10.1093/molbev/msy096 pmid: 29722887
[36] Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69-76.
doi: 10.1007/BF00020088 pmid: 24306565
[37] Zhou Y Y, Zhu H, He S Z, Zhai H, Zhao N, Xing S H, Wei Z H, Liu Q C. A novel sweetpotato transcription factor gen. IbMYB116 enhances drought tolerance in transgenic Arabidopsis. Front Plant Sci 2019, 10: 1025.
[38] Zhang H, Zhang Q, Zhai H, Gao S P, Yang L, Wang Z, Xu Y T, Huo J X, Ren Z T, Zhao N, Wang X F, Li J G, Liu Q C, He S Z. IbBBX24 promotes the jasmonic acid pathway and enhances Fusarium wilt resistance in sweet potato. Plant Cell, 2020, 32: 1102-1123.
[39] Shitamichi N, Matsuoka D, Sasayama D, Furuya T, Nanmori T. Over-expression of MAP3Kδ4, an ABA-inducible Raf-like MAP3K that confers salt tolerance i. Arabidopsis. Plant Biotechnol 2013, 30: 111-118.
[40] Li X, Wang Z, Sun S F, Dai Z R, Zhang J, Wang W B, Peng K, Geng W H, Xia S H, Liu Q C, Zhai H, Gao S P, Zhao N, Tian F, Zhang H, He S Z. IbNIEL-mediated degradation of IbNAC087 regulates jasmonic acid-dependent salt and drought tolerance in sweet potato. J Integr Plant Biol, 2024, 66: 176-195.
doi: 10.1111/jipb.13612
[41] Katiyar A, Smita S, Lenka S K, Rajwanshi R, Chinnusamy V, Bansal K C. Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis. BMC Genomics, 2012, 13: 544.
doi: 10.1186/1471-2164-13-544 pmid: 23050870
[42] Baldoni E, Genga A, Cominelli E. Plant MYB transcription factors: their role in drought response mechanisms. Int J Mol Sci, 2015, 16: 15811-15851.
doi: 10.3390/ijms160715811 pmid: 26184177
[43] Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003, 15: 63-78.
[44] Li X M, Zhong M, Qu L N, Yang J X, Liu X Q, Zhao Q, Liu X M, Zhao X Y.AtMYB32 regulates the ABA response by targeting ABI3, ABI4 and ABI5 and the drought response by targeting CBF4 in Arabidopsis. Plant Sci, 2021, 310: 110983.
[45] Wyrzykowska A, Bielewicz D, Plewka P, Soltys-Kalina D, Wasilewicz-Flis I, Marczewski W, Jarmolowski A, Szweykowska- Kulinska Z. The MYB33, MYB65, and MYB101 transcription factors affect Arabidopsis and potato responses to drought by regulating the ABA signaling pathway. Physiol Plant, 2022, 174: e13775.
[46] Yang A, Dai X Y, Zhang W H. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot, 2012, 63: 2541-2556.
doi: 10.1093/jxb/err431 pmid: 22301384
[47] Tang Y H, Bao X X, Zhi Y L, Wu Q, Guo Y R, Yin X H, Zeng L Q, Li J, Zhang J, He W L, Liu W H, Wang Q W, Jia C K, Li Z K, Liu K. Overexpression of a MYB family gene, OsMYB6, increases drought and salinity stress tolerance in transgenic rice. Front Plant Sci, 2019, 10: 168.
[48] Peng Y, Tang N, Zou J, Ran J, Chen X B. Rice MYB transcription factor OsMYB1R1 negatively regulates drought resistance. Plant Growth Regul, 2023, 99: 515-525.
[49] Chen T Z, Li W J, Hu X H, Guo J R, Liu A M, Zhang B L. A cotton MYB transcription factor, GbMYB5, is positively involved in plant adaptive response to drought stress. Plant Cell Physiol, 2015, 56: 917-929.
[50] Shin D J, Moon S J, Han S, Kim B G, Park S R, Lee S K, Yoon H J, Lee H E, Kwon H B, Baek D, Yi B Y, Byun M O. Expression of StMYB1R-1, a novel potato single MYB-Like domain transcription factor, increases drought tolerance. Plant Physiol, 2011, 155: 421-432.
[51] Li B, Zheng J C, Wang T T, Min D H, Wei W L, Chen J, Zhou Y B, Chen M, Xu Z S, Ma Y Z. Expression analyses of soybean VOZ transcription factors and the role of GmVOZ1G in drought and salt stress tolerance. Int J Mol Sci, 2020, 21: 2177.
[52] Song C, Lee J, Kim T, Hong J C, Lim C O. VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses i. Arabidopsis. Planta 2018, 247: 1439-1448.
[53] Chong L, Xu R, Huang P C, Guo P C, Zhu M K, Du H, Sun X L, Ku L X, Zhu J K, Zhu Y F. The tomato OST1-VOZ1 module regulates drought-mediated flowering. Plant Cell, 2022, 34: 2001-2018.
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