烟草NtWRKY6基因响应ABA表达及其调控多酚合成的功能研究

doi:10.3724/SP.J.1006.2026.54056

摘要/Abstract

摘要：

转录因子WRKY6参与调控植物种子萌发和幼苗生长等过程，但其是否参与调控植物多酚合成未见报道。为探究烟草WRKY6的生物学功能，首先从栽培烟草基因组中鉴定获得一个NtWRKY6基因，序列比对及进化分析发现其编码蛋白具有典型的WRKY家族功能域，属于Group II亚型WRKY类转录因子。表达模式分析显示，NtWRKY6基因在烟草花和蒴果中表达水平较高，且其表达受ABA、BL、JA等激素的显著诱导。过表达NtWRKY6显著增强了烟草幼苗对ABA处理的抗性，且3个独立NtWRKY6-OE株系叶片和根中的多酚含量分别比野生型对照显著增加18.54%~77.15%与39.72%~64.07%；相反地，ntwrky6突变体对ABA处理更加敏感，且2个ntwrky6突变体株系叶片和根的多酚含量分别较野生型对照降低25.05%~30.67%与19.85%~25.06%。与野生型对照相比，NtWRKY6-OE幼苗中多个多酚合成相关基因的表达水平被显著上调，而ntwrky6幼苗中这些基因的表达水平则被显著下调。这些结果表明，NtWRKY6参与调控烟草ABA信号转导及多酚类物质的合成，为烟草品质改良提供了新的靶标基因。

关键词: 烟草, NtWRKY6, 脱落酸, 多酚, 基因表达

Abstract: The transcription factor WRKY6 is known to regulate seed germination and seedling growth in plants; . However, its role in polyphenol biosynthesis has not yet been reported. To investigate the biological function of tobacco WRKY6, the NtWRKY6 gene was identified from the Nicotiana tabacum genome. Sequence alignment and phylogenetic analysis revealed that NtWRKY6 contains a typical WRKY domain and belongs to the Group II subfamily. NtWRKY6 was highly expressed in tobacco flowers and capsules, and its transcription was significantly induced by several plant hormones, including ABA, BL, and JA. Overexpression of NtWRKY6 significantly enhanced the resistance of tobacco seedlings to ABA treatment. In three independent NtWRKY6-overexpressing (OE) lines, polyphenol content in leaves and roots increased by 18.54%–77.15% and 39.72%–64.07%, respectively, compared to wild-type (WT) plants. In contrast, ntwrky6 mutants were more sensitive to ABA treatment, and polyphenol content in their leaves and roots decreased by 25.05%–30.67% and 19.85%–25.06%, respectively, relative to WT plants. Furthermore, the expression levels of multiple genes involved in polyphenol synthesis were significantly upregulated in NtWRKY6-OE seedlings, while they were significantly downregulated in ntwrky6 mutants. These results indicate that NtWRKY6 plays a role in regulating ABA signal transduction and polyphenol biosynthesis, providing a potential target gene for improving tobacco quality.

Key words: tobacco, NtWRKY6, abscisic acid, polyphenol, gene expression

詹戈锐, 余文, 李锋, 武明珠, 徐馨, 罗朝鹏, 巫升鑫, 杨军, 张智强, 王中. 烟草NtWRKY6基因响应ABA表达及其调控多酚合成的功能研究[J]. 作物学报, 2026, 52(2): 446-458.

Zhan Ge-Rui, Yu Wen, Li Feng, Wu Ming-Zhu, Xu Xin, Luo Zhao-Peng, Wu Sheng-Xin, Yang Jun, Zhang Zhi-Qiang, Wang Zhong. Functional study of NtWRKY6 in response to ABA expression and regulation of polyphenol synthesis[J]. Acta Agronomica Sinica, 2026, 52(2): 446-458.

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