欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (07): 1240-1246.

• 耕作栽培·生理生化 • 上一篇    下一篇

矮杆和半矮杆大豆突变体植株生长对外源GA3的响应

李葳;朱保葛*;徐民新;张利明;陈修文;马文平;智艳阳   

  1. 中国科学院遗传与发育生物学研究所, 北京100101
  • 收稿日期:2007-11-19 修回日期:1900-01-01 出版日期:2008-07-12 网络出版日期:2008-07-12
  • 通讯作者: 朱保葛

Responses of Plant Growth of Dwarf and Semi-Dwarf Soybean Mutants to Exogenous GA3

LI Wei,ZHU Bao-Ge*,XU Min-Xin,ZHANG Li-Ming,CHEN Xiu-Wen,MA Wen-Ping,ZHI Yan-Yang   

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2007-11-19 Revised:1900-01-01 Published:2008-07-12 Published online:2008-07-12
  • Contact: ZHU Bao-Ge

PDF

1409

被引次数

12

摘要： 矮杆和半矮杆突变体是研究植物基因功能和培育高产作物新品种的重要材料。本研究以矮杆和半矮杆大豆突变体与各自野生型品种为材料, 利用外源赤霉素(GA3)处理不同发育时期植株, 研究植株生长速率和成熟期株高性状发育对外源GA3的响应及主茎纵向细胞的形态学变化, 分析两类突变体与各自野生型之间的内源赤霉素含量差异。结果表明, 外源GA3(40 mg L-1)对半矮杆和矮杆突变体的作用效果明显不同, GA3处理后半矮杆突变体植株在不同测定时段的平均生长速率达到2.76 cm d-1, 而矮杆突变体的平均生长速率仅为0.92 cm d-1, 而且成熟期半矮杆突变体植株的平均高度、主茎平均节间长度与节间纵向细胞长度均显著超过对照, 外源GA3能够恢复其正常的野生型株高表型; 而矮杆突变体较对照的上述3项增幅均明显小于半矮杆型, 外源GA3不能恢复其正常的野生型株高表型。说明半矮杆突变体的植株生长对外源GA3有明显响应, 为GA3敏感型突变体; 而矮杆突变体仅在生育前期对外源GA3有一定程度响应, 为GA3钝感型突变体。半矮杆和矮杆突变体成熟期植株的内源GA3+1含量均显著低于各自野生型品种, 这可能是二者表型矮化的生理原因。

关键词: 大豆, 矮杆/半矮杆突变体, 赤霉素, 植株生长, 株高性状

Abstract: Dwarf and semi-dwarf mutants are the valuable materials for investigating plant gene function and developing new crop variety with high seed yield and lodging resistance. In the present study, the developing plants of dwarf (df) and semi-dwarf (sdf) mutants and their wild-types (WTs) in different growth stages were treated with exogenous gibberellin A3 (GA3) in order to analyze responses of plant growth rate, plant height, and vertical cell length from the main stem to exogenous GA3 (40 mg L-1), as well as the endogenous GA3+1 level differences between two types of mutants and their WTs. The results indicated that the responses of plant growth of the sdf and df mutants to the exogenous GA3 were significantly different, demonstrating that GA3 treatment led to the average rate (2.76 cm d-1) of plant growth of the sdf mutant in different measuring-time intervals to be significantly higher than that (1.10 cm d-1) of water treatment (control); but the average rate of plant growth (0.92 cm d-1) of GA3-treated df mutant was slightly higher than that (0.56 cm d-1) of control, and moreover df mutant plants in the prophase of entire growth period only had a little response to exogenous GA3. In addition, after GA3 treatment the average plant height, internode length, and vertical cell length of the internode on the main stem of sdf mutant were much higher than those of df mutant, verifying the exogenous GA3 treatment could recover plant height phenotype of WT-sd in sdf mutant, while not recover that of WT-d in df mutant. The node number on the main stem of two types of mutants was almost no response to GA3. Based on above results we think that sdf is a GA3-sensitive soybean mutant, and df is a GA3-insensitive soybean mutant. Furthermore it was found that the endogenous GA1+3 levels in sdf and df mutant plants were remarkably lower than that in their WT plants, which may be the physiological cause of dwarfism for sdf and df mutants.

Key words: Glycine max (L.), Dwarf/semi-dwarf mutant, Gibberellin, Plant growth, Plant height trait

中图分类号: 

  • 10.3724/SP.J.1006.2008.01240
[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[3] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[4] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[5] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[6] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[7] 周慧文, 丘立杭, 黄杏, 李强, 陈荣发, 范业赓, 罗含敏, 闫海锋, 翁梦苓, 周忠凤, 吴建明. 甘蔗赤霉素氧化酶基因ScGA20ox1的克隆及功能分析[J]. 作物学报, 2022, 48(4): 1017-1026.
[8] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[9] 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895.
[10] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[11] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[12] 周悦, 赵志华, 张宏宁, 孔佑宾. 大豆紫色酸性磷酸酶基因GmPAP14启动子克隆与功能分析[J]. 作物学报, 2022, 48(3): 590-596.
[13] 王娟, 张彦威, 焦铸锦, 刘盼盼, 常玮. 利用PyBSASeq算法挖掘大豆百粒重相关位点与候选基因[J]. 作物学报, 2022, 48(3): 635-643.
[14] 董衍坤, 黄定全, 高震, 陈栩. 大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能[J]. 作物学报, 2022, 48(2): 353-366.
[15] 张国伟, 李凯, 李思嘉, 王晓婧, 杨长琴, 刘瑞显. 减库对大豆叶片碳代谢的影响[J]. 作物学报, 2022, 48(2): 529-537.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2