碱胁迫下粳稻幼苗前期耐碱性的数量性状基因座检测

doi:10.3724/SP.J.1006.2009.00301

作物学报 ›› 2009, Vol. 35 ›› Issue (2): 301-308.doi: 10.3724/SP.J.1006.2009.00301

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

碱胁迫下粳稻幼苗前期耐碱性的数量性状基因座检测

祁栋灵^1,2;郭桂珍³;李明哲⁴;杨春刚³;张俊国³;曹桂兰¹;张三元³

1中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种持资源与生物技术重点开放实验室，北京100081；2中国热带农业科学院橡胶研究所/农业部热带作物栽培生理学重点开放实验室，海南儋州571737；3吉林省农业科学院水稻研究所，吉林公主岭136100；4韩国农村振兴厅农业生命工学研究院，韩国水原441-707；5四川农业大学园艺系，四川雅安625014

收稿日期:2008-08-15 修回日期:2008-10-05 出版日期:2009-02-12 网络出版日期:2008-12-11
通讯作者: 韩龙植
基金资助:
本研究由国家科技支撑计划项目（2006BAD13B01），国家重点基础研究发展计划（973计划）项目（2004CB117201），中韩合作项目（2006-2009）资助。

Identification of Quantitative Trait Loci for Alkaline Tolerance at Early Seedling Stage under Alkaline Stress in Japonica Rice

QI Dong-Ling^1,2,GUO Gui-Zhen³,LEE Myung-Chul⁴,YANG Chun-Gang³,ZHANG Jun-Guo³,CAO Gui-Lan¹,ZHANG San-Yuan³,SUH Seok-Cheol⁴,ZHOU Qing-Yang⁵,HAN Long-Zhi^1,*

1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Beijing 100081,China;2rubber Research Institute of China, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Physiology for Tropical Crops, Ministry of Agriculture, Danzhou 571737,China;3Institute of Rice Research,Jilin Academy of Agricultural Sciences, Gongzhuling 136100,China;4 National Institute of Agricultural biotechnology, Rural Development Administration, Suwon 441-707,Korea;5 Horticultural Department, Sichuan Agricultural University,Ya'an 625014,China

Received:2008-08-15 Revised:2008-10-05 Published:2009-02-12 Published online:2008-12-11
Contact: HAN Long-Zhi

摘要/Abstract

摘要：

以粳粳交“高产106/长白9号”F_2:3代200个家系为作图群体, 在0.15% Na₂CO₃溶液的碱性胁迫下, 进行了水稻耐碱性鉴定, 并以SSR标记构建的分子连锁图谱为基础, 对水稻幼苗前期的根数、根长和苗高及其相对碱害率进行了数量性状基因座(QTLs)的检测。结果表明, 上述性状在F₃家系群中均表现为具有1~2个峰的连续分布, 认为由主效基因和微效基因共同控制的数量性状。共检测到与碱胁迫下幼苗前期根数、根长和苗高及其相对碱害率相关的QTL 26个, 分布于第1、5、6、7、8、9和11染色体上。其中, 碱胁迫下与根数相关的QTL 4个, qRN6-1和qRN11对表型变异的解释率较大, 分别为29.91%和13.42%；与根数相对碱害率相关的QTL 5个, qRRN11-2对表型变异的解释率较大, 为23.86%；与根长相关的QTL 6个, qRRL11-2对表型变异的解释率较大, 为21.06%；与根长相对碱害率相关的QTL 2个, 但对表型变异的解释率均较低；与苗高相关的QTL 5个, qSH1和qSH11-2对表型变异的解释率较大, 分别为15.81%和16.53%；与苗高相对碱害率相关的QTL 4个, qRSH5和qRSH6-2对表型变异的解释率分别为29.89%和34.63%。而这些解释率较大的QTL所处的标记区间距离, 除qRN6-1相对较小(19.0 cM)外, 其余QTL的标记区间距离均大于26.3 cM, 需作进一步的精细定位。在所检测到的QTL中, 13个QTL的增效等位基因均来自耐碱亲本长白9号, 而其余QTL的增效等位基因来自敏碱亲本高产106；基因的主要作用方式为超显性或部分显性。

关键词: 水稻, 幼苗前期, 耐碱性, 微卫星标记, 数量性状基因座

Abstract:

The quantitative trait loci (QTLs) for root number, root length, seedling height and their relative alkaline damage rates at early seedling stage in rice under 0.15% Na₂CO₃alkaline stress were identified using a F_2:3 population, which included 200 individuals and lines derived from a cross between two japonica rice cultivars Gaochan 106 and Changbai 9 with microsatellite markers. All the traits above showed a continuous distribution with 1 or 2 peaks in F₃lines under alkaline stress, which were the quantitative traits controlled by major and multiple QTLs. Twenty-six QTLs associated with root number, root length and seedling height and their relative alkaline damage rates at early seedling stage under alkaline stress were detected, and which were located on chromosomes 1, 5, 6, 7, 8, 9, and 11. These QTLs included 4 QTLs associated with root number, 5 QTLs correlated with relative alkaline damage rate for root number, 6 QTLs controlling root length, 2 QTLs for relative alkaline damage rate for root length, 5 QTLs associated with seedling height, 4 QTLs related to relative alkaline damage rate for seedling height. qRN6-1, qRN11, qRRN11-2, qRRL11-2, qSH1, qSH11-2, qRSH5, and qRSH6-2, accouonted for 29.91, 13.42, 23.86, 21.06, 15.81, 16.53, 29.89, and 34.63% of the observed phenotypic variation, respectively. While the marker flanking distance of these QTLs explained larger. The observed phenotypic variation were more than 26.3 cM, except qRN6-1 with 19.0 cM of marker flanking distance, which should be conducted more refined mapping further. Of detected QTLs, the alleles for 13 QTLs originated from the tolerant parent Changbai 9, and the alleles of other QTLs were derived from alkaline sensitive parent Gaochan 106. These gene actions showed mainly part dominance or over dominant effects.

Key words: Rice, Alkaline tolerance, Early seedling stage, Microsatellite marker, Quantitative trait locus(QTL)

祁栋灵;郭桂珍;李明哲;杨春刚;张俊国;曹桂兰;张三元. 碱胁迫下粳稻幼苗前期耐碱性的数量性状基因座检测[J]. 作物学报, 2009, 35(2): 301-308.

QI Dong-Ling,GUO Gui-Zhen,LEE Myung-Chul,YANG Chun-Gang,ZHANG Jun-Guo,CAO Gui-Lan,ZHANG San-Yuan,SUH Seok-Cheol,ZHOU Qing-Yang,HAN Long-Zhi. Identification of Quantitative Trait Loci for Alkaline Tolerance at Early Seedling Stage under Alkaline Stress in Japonica Rice[J]. Acta Agron Sin, 2009, 35(2): 301-308.

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碱胁迫下粳稻幼苗前期耐碱性的数量性状基因座检测

Identification of Quantitative Trait Loci for Alkaline Tolerance at Early Seedling Stage under Alkaline Stress in Japonica Rice

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