欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1236-1243.doi: 10.3724/SP.J.1006.2009.01236

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于大豆胞囊线虫抗病候选基因rhg1的InDe1标记发掘与鉴定

南海洋,李英慧**,常汝镇,邱丽娟*   

  1. 中国农业科学院作物科学研究所/高家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源利用重点开放实验室，北京100081
  • 收稿日期:2008-10-28 修回日期:2009-03-16 出版日期:2009-07-12 网络出版日期:2009-05-19
  • 通讯作者: 邱丽娟, E-mail: qiu_lijuan@263.net
  • 作者简介:E-mail: haiyang_mei@163.com
  • 基金资助:

    本研究由国家自然科学基金项目（30490251和30471096），高家高技术研究发展计划（863计划）项目（2006AA100104，2006AA10Z164，2006AA10Z1B3)，高家“十一五”科技支撑计划项目（2006BAD13B05），国际科技合作项目（2008DFA30550），中国农业科学院作物科学研究所科研基金资助。

Development and Identification of InDel Marker Based on rhg1 Gene for Resistance to Soybean Cyst Nematode(Heterodera glycines Ichinohe)

NAN Hai-Yang,LI Ying-Hui**,CHANG Ru-Zhen,QIU Li-Juan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Germplasm & Biotechnology/Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，Beijing 100081，China
  • Received:2008-10-28 Revised:2009-03-16 Published:2009-07-12 Published online:2009-05-19
  • Contact: QIU Li-Juan, E-mail: qiu_lijuan@263.net
  • About author:E-mail: haiyang_mei@163.com

PDF

2312

被引次数

15

摘要：

大豆胞囊线虫病是严重危害大豆生产的重要病害之一，根据抗病候选基因发掘标记可以为分子标记辅助选择抗病材料提供标记资源。本研究通过对大豆胞囊线虫抗病候选基因rhg1的序列比对分析，发现4个插入/删除位点，针对其中3个多碱基插入/缺失位点开发了InDel标记。应用开发的3个InDel标记对33份栽培大豆进行基因型鉴定，共检测到等位变异11个，平均每个位点3.67个。其中rhg1-I1位点有等位变异5个，rhg1-I2位点有等位变异2个；rhg1-I4位点有等位变异4个。各等位变异发生频率范围为0.8%~77.3%。InDel标记与大豆胞囊线虫抗性间的关联分析表明，rhg1-I4为抗性相关标记，对抗病资源的检出效率为88.2%，对感病资源的检出效率为100%。该标记的288 bp等位变异和294 bp等位变异为抗病相关等位变异，269 bp等位变异和272 bp等位变异为感病相关等位变异。此标记与常用于标记辅助选择的Satt309配合鉴定可以提高SCN抗病资源的检测效率。

关键词: 大豆, 胞囊线虫病, InDel标记

Abstract:

Soybean cyst nematode (Heterodera glycaines, SCN) is one of the most important pests of soybean in the world. New molecular markers developed from resistance genes can provide marker resources for MAS (marker-assisted selection) breeding. Among four InDel loci detectedby comparing thesequences of candidate rhg1 conferringSCN resistance, three multiple-bases InDel loci were used to develop markers. A total of 11 alleles were detected in 33 cultivated soybean germplasm using three InDel markers developed. There were 5 alleles at rhg1-I1 locus, 2 and 4 at rhg1-I2 and rhg1-I4 respectively. Allelic variations at three InDel markers were assayed in the genotypes and discovered that rhg1-I4 marker was related to SCN resistance, which could distinguish 88.2% of resistant germplasm and 100% of the susceptible germplasm. Alleles of 288 and 294 bp presented in the SCN-resistant germplasm, and alleles 269 and 272 bp presented in the SCN-susceptible germplasm. In addition, rhg1-I4 could be used in MAS for the progenies of cross between typical southern US cultivar Lee crossed and the important resistant source PI88788. Combing with Satt309 which displayed the identical allele on these genotypes, rhg1-I4 would be higher effective in identifying germplasm with rhg1 conferring resistance in the MAS breeding.

Key words: Soybean, SCN, InDel marker

[1] Liu W-Z(刘维志), Liu Y(刘晔), Chen P-S(陈品三). Preliminary report of identify the races of SCN in some city & county of Northeast. J Shenyang Agric Coll (沈阳农学院学报), 1984, (2): 74-78(in Chinese)

[2] Liu H-Q(刘汉起), Shang S-G(商绍刚), Huo H(霍虹), Wu H-L(吴和礼). Resistance of soybean varieties to race 1,3 and 4 of soybean cyst nematode. Soybean Sci (大豆科学), 1989, 8(1): 113-114(in Chinese)

[3] Lu W-G(卢为国), Gai J-Y(盖钧镒), Li W-D(李卫东). Sample survey and identification of races of soybean cyst nematode (Heterodera glycines Ichinohe) in Huang-Huai valley. Sci Agric Sin (中国农业科学),2006, 39(2): 306-312(in Chinese with English abstract)

[4] Shang S-G(商绍刚), Liu H-Q(刘汉起). The distribution of races of soybean cyst nematode (Heterodera glycines Ichinohe) in Dongbei. Soybean Sci (大豆科学), 1989, 8(4): 382(in Chinese)

[5] Liu P-Y(刘佩印). Advances in study of screening and utilization for antigen to soybean cyst nematode. Heilongjiang Agric Sci (黑龙江农业科学), 2005, (6): 44-47 (in Chinese with English abstract)

[6] Riggs R D, Schmidt D P. Complete characterization of the race scheme for Heterodera glycines. J Nematol, 1988, 20: 392-395

[7] Caviness C E. Breeding of Resistance to Soybean Cyst Nematode. In: Riggs R D, Wrather J A, eds. Biology and Management of the Soybean Cyst Nematode. St. Paul, Minnesota: APS Press, 1992. pp 143-156

[8] Cregan P B, Mudge J, Fickus E W, Denny R, Danesh R, Young N D. Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by the rhg1 locus. Theor Appl Genet, 1999, 99: 811-818

[9] Arelli A P, Anand S C, Wrather J A. Soybean resistance to soybean cyst nematode race 3 is conditioned by an additional dominant gene. Crop Sci, 1992, 32: 862-864

[10] Concibido V C, Diers B W, Arelli P R. A decade of QTL mapping for cyst nematode resistance in soybean. Crop Sci, 2004, 44: 1121-1131

[11] Ruben E, Aziz J, Afzal J, Njiti V N, Triwitayakorn K, Iqbal M J, Yaegashi S, Arelli P, Town C, Meksem K, Lightfoot D A. Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode. Mol Genet Genome, 2006, 276: 503-516

[12] Concibido V C, Denny R L, Boutin S R, Hautea R, Orf J H, Young N D. DNA marker analysis of loci underlying resistance to soybean cyst nematode (Heterodera glycines Ichinohe). Crop Sci, 1994, 34: 240-246

[13] Concibido V C, Boutin S, Denny R L, Hautea R, Orf J, Young N D. Targeted comparative genome analysis and qualitative mapping of a major partial resistance gene to the soybean cyst nematode. Theor Appl Genet, 1996, 93: 234-241

[14] Webb D M, Baltazar B M, Arelli A P, Schupp J, Keim P, Clayton K, Ferreira A R, Owens T, Beavis W D. QTL affecting soybean cyst nematode resistance. Theor Appl Genet, 1995, 91: 574-581

[15] Chang S J C, Doubler T W, Kilo V, Suttner R J, Klein J, Schmidt M E, Gibson P T, Lightfoot D A. Association of loci underlying field resistance to soybean sudden death syndrome (SDS) and cyst nematode (SCN) race 3. Crop Sci, 1997, 372: 965-971

[16] Prabhu R R, Njiti V, Bell-Johnson B, Johnson J E, Schmidt M E, Klein J, Lightfoot D A. Selecting soybean cultivars for dual resistance to soybean cyst nematode and sudden death syndrome using two DNA markers. Crop Sci, 1999, 39: 982-987

[17] Meksem K, Ruben E, Hyten D L, Schmidt M E, Lightfoot D A. High-throughput genotyping for a polymorphism linked to soybean cyst nematode resistance gene Rhg4 by using Taqman (TM) probes. Mol Breed, 2001, 7: 63-71

[18] Yue P, Sleper D A, Arelli P R. Mapping resistance of multiple races of Heterodera glycines in soybean PI89772. Crop Sci, 2001, 41: 1589-1595

[19] Guo B, Sleper D A, Nguyen H T, Arelli P R, Shannon J G. Quantitative trait loci underlying resistance to three soybean cyst nematode populations in soybean PI404198A. Crop Sci, 2006, 46: 224-233

[20] Goffinet B, Gerber S. Quantitative trait loci: A meta-analysis.Genetics, 2000, 155: 463-473

[21] Guo B, Sleper D A, Lu P, Shannon J G, Nguyen H T, Arelli P R. QTLs associated with resistance to soybean cyst nematode in soybean: Meta-analysis of QTL locations. Crop Sci, 2006, 46: 595-602

[22] Lightfoot D A, Meksem K. Novel polynucleotides and polypeptides relating to loci underlying resistance to soybean cyst nematode and methods of use thereof. Patent pending # 09/772, 134. Filing date 2000-01-29

[23] Hauge B M, Wang M L, Parsons J D, Parnell L D. Nucleic acid molecules and other molecules associated with soybean cyst nematode resistance. WO 01/51627 PCT/US01/00552 Patent # 20030005491

[24] Concibido V C, Lange D A, Denny R L, Hautea R, Orf J, Young N D. Genome mapping soybean cyst nematode resistance genes in Peking, PI90763 and PI88788 using DNA markers. Crop Sci, 1997, 37: 258-264

[25] Heer J A, Knap H T, Mahalingam R, Shipe E R, Arelli P R, Matthews B F. Molecular markers for resistance to Heterodera glycines in advanced soybean germplasm. Mol Breed, 1998, 4: 359-367

[26] Meksem K, Pantazopoylos P, Niti V N, Hyten L D, Arelli P R. ‘Forrest’ resistance to soybean cyst nematode is bigenic: Saturation mapping of the Rhg1 and Rhg4 loci. Theor Appl Genet, 2001, 103: 710-714

[27] Mudege J, Cregan P B. Two microsatellite markers that flank the major soybean cyst nematode resistance locus. Crop Sci, 1997, 37: 1611-1615

[28] Qiu B X, Arelli P R, Sleper D A. RFLP markers associated with soybean cyst nematode resistance and seed composition in a ‘Peking’× ‘Essex’ population. Theor Appl Genet, 1999, 98: 356-364

[29] Ma Y S, Wang W H, Wang L X, Ma F M, Wang P W, Chang R Z, Qiu L J. Genetic diversity of soybean and the establishment of a core collection focused on resistance to soybean cyst nematode. J Integr Plant Biol, 2006, 48: 722-731

[30] Liu M S, Amirkhanian V D. DNA fragment analysis by an affordable multiple-channel capillary electrophoresis system (Short communication). Electrophoresis-Weinheim, 2003, 24: 93-95

[31] Mills R E, Luttig C T, Larkins C E, Beauchamp A, Tsui C, Pittard W S, Devine S E. An initial map of insertion and deletion (INDEL) variation in the human genome. Genome Res,2006, 16: 1182-1190

[32] Shen Y J, Jiang H, Jin J P, Zhang Z B, Xi B, He Y Y, Wang G, Wang C, Lily Q, Yu Q B, Liu H J, Chen D H, Gao J H, Huang H, Shi T L, Yang Z N. Development of genome-wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiol, 2004, 135: 1198-1205

[33] Feltus F A, Wan J, Schulze S R, Wan J, Estill J C, Jiang N, Paterson A H. An SNP resource for rice genetics and breeding based on subspecies indica and japonica genome alignments. Genome Res, 2004, 14: 1812-1819

[34] Dinakar B, Maureen D, Mike H, Robin W, Dave V, James C R. Insertion-deletion polymorphisms in 3' regions of maize genes occur frequently and can be used as highly informative genetic markers. Plant Mol Biol, 2002, 48: 539-547

[35] Choi I Y, Hyten D L, Matukumalli L K, Song Q, Chaky J M, Quigley C V, Chase K, Lark K G, Reiter R S, Yoon M S, Hwang E Y, Yi S I, Young N D, Shoemaker R C, Van Tassell C P, Specht J E, Cregan P B. A soybean transcript map: Gene distribution, haplotype and SNP analysis. Genetics, 2007, 176: 685-696

[36] Ellegren H. Microsatellites: Simple sequences with complex evolution. Nat Rev Genet, 2004, 5: 435-445

[37] Li Y H, Guan R X, Ma Y S, Wang L X, Li L H, Lin F Y, Luan W J, Chen P Y, Yan Z, Guan Y, Zhu L, Ning X C, Smulders M J M, Li W, Piao R H, Cui Y H, Yu Z M, Guan M, Chang R Z, Liu Z X, Hou A F, Shi A N, Zhang B, Zhu S L, Qiu L J. Genetic structure and diversity of cultivated soybean

[Glycine max (L.) Merr.] landraces in China. Theor Appl Genet, 2008, 117: 857-871

Feng F-J(冯芳君), Luo L-J(罗利军), Li-Y(李荧), Zou L-G(周立国), Xu X-Y(徐小艳), Wu J-H(吴金红), Chen H-W(陈宏伟), Chen L(陈亮), Mei H-W(梅捍卫). Comparative analysis of polymorphism of InDel and SSR markers in rice. Mol Plant Breed (植物分子育种), 2005, 3(5): 725-730(in Chinese with English abstract)
[1] 金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829.
[2] 唐宽强, 李公允, 宋美毅, 赵雪, 常春玲. 大豆株高性状全基因组关联分析及预测模型构建[J]. 作物学报, 2026, 52(6): 1743-1756.
[3] 姚术, 郭凯悦, 翟慧慧, 姚佳慧, 邓文琪, 闫玲, 黄驰, 高阳, 俞嫣然, 赵振邦, 李英慧, 王晓波, 李佳佳. 大豆苗期耐低铁综合评价及优异种质筛选[J]. 作物学报, 2026, 52(5): 1373-1387.
[4] 张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493.
[5] 贺红利, 张雨涵, 杨静, 程云清, 赵杨, 李星诺, 司洪亮, 张兴政, 杨向东. 大豆e1-as基因突变体的创制及生理分析[J]. 作物学报, 2025, 51(8): 2228-2239.
[6] 王克晶, 李向华. 我国珍稀的大豆属多年生烟豆和短绒野大豆物种遗传资源濒危性评估分析[J]. 作物学报, 2025, 51(8): 2009-2019.
[7] 孟然, 李赵嘉, 冯薇, 陈悦, 刘路平, 杨春燕, 鲁雪林, 王秀萍. 大豆不同生育时期耐盐性综合评价及耐盐种质筛选[J]. 作物学报, 2025, 51(8): 1991-2008.
[8] 胡蒙, 沙丹, 张晟瑞, 谷勇哲, 张世碧, 李静, 孙君明, 邱丽娟, 李斌. 大豆分枝数QTL定位及候选基因筛选[J]. 作物学报, 2025, 51(7): 1747-1756.
[9] 王琼, 邹丹霞, 陈兴运, 张威, 张红梅, 刘晓庆, 贾倩茹, 魏利斌, 崔晓艳, 陈新, 王学军, 陈华涛. 大豆开花时间和成熟期性状全基因组关联分析与候选基因预测[J]. 作物学报, 2025, 51(6): 1558-1568.
[10] 殷丛丛, 李睿琦, 岳霈尧, 李晨, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 基于闭合哑铃介导等温扩增可视化检测大豆花叶病毒SC15方法的建立及应用[J]. 作物学报, 2025, 51(5): 1248-1260.
[11] 许睿, 何妙华, 王昊, 李卫, 任杰, 夏志强. 基于空间转录组技术解析大豆种胚对X射线辐射的响应机制[J]. 作物学报, 2025, 51(12): 3121-3132.
[12] 林洋, 史晓蕾, 陈强, 刘兵强, 杨庆, 于慧娟, 闫龙, 武小霞, 杨春燕. 大豆蛋白质脂肪及脂肪酸组分相关QTL定位[J]. 作物学报, 2025, 51(11): 2899-2910.
[13] 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[14] 李威, 朱玉鹏, 孙宾成, 温有祥, 吴宗声, 徐一帆, 宋雯雯, 徐彩龙, 吴存祥. 转基因大豆结合免耕平作实现东北地区大豆生产轻简化[J]. 作物学报, 2025, 51(10): 2738-2749.
[15] 陈敏, 贾蓉, 张金传, 张辰煜, 褚俊聪, 姚伟, 葛军勇, 王星宇, 杨亚东, 曾昭海, 臧华栋. 半干旱区燕麦与豆科作物带状复合种植的产量优势及氮素利用特征研究[J]. 作物学报, 2025, 51(10): 2727-2737.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2