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作物学报 ›› 2012, Vol. 38 ›› Issue (06): 1018-1028.doi: 10.3724/SP.J.1006.2012.01018

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黄淮冬麦区175个小麦品种的SSR多态性及其与株高、产量相关性状的关联分析

武玉国，吴承来，秦保平，王振林，黄玮，杨敏，尹燕枰   

  1. 作物生物学国家重点实验室 / 山东省作物生物学重点实验室 / 山东农业大学农学院, 山东泰安  271018
  • 收稿日期:2011-11-28 修回日期:2012-01-19 出版日期:2012-06-12 网络出版日期:2012-03-05
  • 通讯作者: 尹燕枰, E-mail: ypyinsdau@sina.com, Tel: 0538-8242458
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(201203029)，国家转基因生物新品种培育重大专项(2009ZX08002-004B)和国家重点基础研究计划(973计划)项目(2009CB118602)资助。

Diversity of SSR Marker in 175 Wheat Varieties from Huang-Huai Winter Wheat Region and Its Association with Plant Height and Yield Related Traits

WU Yu-Guo,WU Cheng-Lai,QIN Bao-Ping,WANG Zhen-Lin,HUANG Wei,YANG Min,YIN Yan-Ping*   

  1. State Key Laboratory of Crop Biology / Shandong Provincial Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China
  • Received:2011-11-28 Revised:2012-01-19 Published:2012-06-12 Published online:2012-03-05
  • Contact: 尹燕枰, E-mail: ypyinsdau@sina.com, Tel: 0538-8242458

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摘要： 为了解小麦品种资源的遗传多样性, 筛选株高、产量相关性状相关标记的等位变异, 选用108对覆盖小麦各同源染色体且多态性高的SSR引物, 对黄淮麦区175个小麦品种进行分析。共检测到448个等位变异, 平均每个标记4.15个等位变异, 变化范围为2~14个；全部SSR位点的多态性信息含量(PIC)变化范围为0.075~0.869, 平均为0.561。聚类分析显示同一地区或同一育种单位育成的、具有共同亲本的品种多数聚为一类。关联分析表明, 与株高、产量相关性状显著关联(P<0.01)的标记有23个, 其中3个标记达到极显著(P<0.001)水平。标记wmc128(1B)和wmc236(3B)与小穗数极显著相关, 分别解释小穗数变异的10.5%和8.0%；标记Xgwm129(2B)与千粒重达到极显著相关, 可以解释千粒重变异的19.3%。

关键词: 小麦, 遗传多样性, SSR, 等位变异, 关联分析

Abstract: To make advances in wheat breeding, it is important to study germplasm diversity of Huang-huai winter wheat, and identify SSR markers associated with important agronomic traits, including plant heightand yield related traits. In this study, we selected 175 historical winter wheat varieties planted in Huang-Huai winter wheatarea and 108 pairs of SSR markers evenly distributed on the chromosomes of wheat to reveal the genetic diversity. A total of 448 alleles of loci were detected in the experimental varieties. The average alleles per locus were 4.15, which varied from 2 to 14. Thepolymorphism information content (PIC) value ranged from 0.075 to 0.869, with an average of 0.560. Cluster analysis showed that most of the varieties with the common parents, bred by the same breeders and planted in the same area were clustered together. A total of 23 were markers significantly (P<0.01) associated with plant height and yield related traits, among which threewere very significantly(P<0.001) associated with the traits.The markerswmc128 (1B) and wmc236 (3B) could explain 10.5% and 8.0% of variation in the number of spikelets(P<0.001), respectively.And, Xgwm129 (2B) was significantly (P<0.001) associated with 1000- kernel weight, whose phenotypic variation explaining rate was as high as 19.3%.

Key words: Wheat, Genetic diversity, SSR, Allele, Association analysis

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