源于栽培种花生的EST-SSR引物对野生花生扩增的多态性

doi:10.3724/SP.J.1006.2012.01221

作物学报 ›› 2012, Vol. 38 ›› Issue (07): 1221-1231.doi: 10.3724/SP.J.1006.2012.01221

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

源于栽培种花生的EST-SSR引物对野生花生扩增的多态性

唐梅^1,2，陈玉宁²，任小平²，黄莉²，周小静²，严海燕¹，姜慧芳^2,*

1 中南民族大学生命科学学院，湖北武汉430074；2 中国农业科学院油料作物研究所 / 农业部油料作物生物学与遗传育种重点实验室，湖北武汉430062

收稿日期:2012-01-13 修回日期:2012-04-16 出版日期:2012-07-12 网络出版日期:2012-05-11
通讯作者: 姜慧芳, E-mail: peanutlab@oilcrops.cn
基金资助:
本研究由国家重点基础研究发展计划(973计划)项目(2011CB109300)和国家自然科学基金项目(31000724)，农作物种质资源保护项目(NB2010-2130135-28B)和国家现代农业产业技术体系建设项目(CARS-14-种质资源评价)资助。

Genetic Diversity of Arachis Accessions Detected by EST-SSR from Cultivated Peanut (Arachis hypogaea L.)

TANG Mei^1,2,CHEN Yu-Ning²,REN Xiao-Ping²,HUANG Li²,ZHOU Xiao-Jing²,YAN Hai-Yan¹,JIANG Hui-Hang^2,*

1 College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China; 2 Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China

Received:2012-01-13 Revised:2012-04-16 Published:2012-07-12 Published online:2012-05-11
Contact: 姜慧芳, E-mail: peanutlab@oilcrops.cn

摘要/Abstract

摘要： 以花生属86份野生近缘种和3份栽培种为材料，利用从栽培种花生中开发设计的EST-SSR引物，分析其对野生花生扩增的有效性，探讨EST-SSR引物用于花生资源遗传多样性研究的适用性。随机选取235对EST-SSR引物进行筛选，223对EST-SSR引物均扩增出条带，有效性为94.89%，其中能检测到多态性的53对引物在89份资源中共扩增出238条带，包括206条多态性带。每对引物能扩增出1~12多态性带，平均3.89条，多态性指数为0.044~4.040，平均1.173。统计分析结果表明，89份花生种质材料间的平均相似系数为0.685，变异范围为0.442~0.976，在遗传距离为0.408处，分成2大组(A组和B组) 9小组，栽培种花生被聚在花生区组中，相同区组的材料基本被聚在一起，A. duranensis与栽培种花生(A. hypogaea L.)的关系较近，聚类结果与花生属的植物学分类基本一致。对含油量和基因型数据相关性分析和t检验发现，POCR437-180/170等6对标记可以作为含油量相关分析标记筛选的后备标记。用这6对标记的引物序列搜索cDNA文库和BLAST库，发现引物POCR437序列对应丙二酸单酰-CoA-ACP转酰酶和酰基载体蛋白的编码基因，这两种蛋白质参与脂肪酸的合成。

关键词: 野生花生, EST-SSR, 有效性, 聚类分析

Abstract: The amplification efficiency and application potential for the research on peanut genetic diversity by using cultivars-derived EST-SSR primers in 89 Arachis aeecssions were assessed. Based on randomly selected 235 EST-SSR primer pairs, we detected 223 amplification bands in the 89 accessions with the amplification efficiency of 94.89%. Among them 53 primer pairs exhibited 206 polymorphic bands, which showed 1–12 bands per primer pair with an average of 3.89. The polymorphism index ranged from 0.044 to 4.040 with an average of 1.173. The average similarity coefficient was 0.685 among 89 peanut accessions, ranging from 0.442 to 0.976. The accessions from the same section were mainly clustered together, peanut cultivars were grouped into the section of Arachis, and A. duranensis had a close relation to cultivated peanut, which is coincident with botanical classification. According to the statistical analysis from the results of oil content and SSR data in wild peanuts, some interesting bands such as POCR437-180/170 were found to be potential for candidate markers related to oil content. BLAST analysis indicated that the alignment sequence of primer POCR437 corresponded to the coding genes of malonyl-CoA-ACP transacylase and acyl-carrier-protein, both of which are involved in fatty acid synthesis.

Key words: Wild peanut, EST-SSR, Effectiveness, Cluster analysis

唐梅，陈玉宁，任小平，黄莉，周小静，严海燕，姜慧芳. 源于栽培种花生的EST-SSR引物对野生花生扩增的多态性[J]. 作物学报, 2012, 38(07): 1221-1231.

TANG Mei,CHEN Yu-Ning,REN Xiao-Ping,HUANG Li,ZHOU Xiao-Jing,YAN Hai-Yan,JIANG Hui-Hang. Genetic Diversity of Arachis Accessions Detected by EST-SSR from Cultivated Peanut (Arachis hypogaea L.)[J]. Acta Agron Sin, 2012, 38(07): 1221-1231.

参考文献

[1]Pande S, Narayana Rao J. Resistance of wild Arachis species to late leaf spot and rust in greenhouse trials. Plant Dis, 2001, 85: 851–855

[2]Johnson D R, Wynne J C, Campbell W V. Resistance of wild species of Arachis to the two spotted spider mite, Tetranychus urticae. Peanut Sci, 1977, 4: 9–11

[3]Hou H-M(侯慧敏), Liao B–S(廖伯寿), Lei Y(雷永), Ren X-P(任小平), Wang S-Y(王圣玉), Li D(李栋), Jiang H-F(姜慧芳), Huang J-Q(黄家权), Chen B-Y(陈本银). Identification of AFLP markers for resistance to peanut rust. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(2): 89–92 (in Chinese with English abstract)

[4]Cao L-K(曹林奎), Wu A-Z(吴爱忠), Ma X-D(马晓冬). Nutritional components analysis of perennial wild species of Arachis L. J Shanghai Agric Coll (上海农学院学报), 1994, 12(2): 140–143 (in Chinese with English abstract)

[5]Jiang H-F(姜慧芳), Ren X-P(任小平), Wang S-Y(王圣玉), Huang J-Q(黄家权), Lei Y(雷永), Liao B-S(廖伯寿). Identification and evaluation of high oil content in wild Arachis species. Chin J Oil Crop Sci (中国油料作物学报), 2010, 32(1): 30–34 (in Chinese with English abstract)

[6]Jiang H-F(姜慧芳), Ren X-P(任小平), Huang J-Q(黄家权), Lei Y(雷永), Liao B-S(廖伯寿). Genetic variation of fatty acid components in Arachis species and development of interspecific hybrids with high oleic and low palmitic acids. Acta Agron Sin (作物学报), 2009, 35(1): 25−32 (in Chinese with English abstract)

[7]Yang X-Q(杨新泉), Liu P(刘鹏), Han Z-F(韩宗福), Ni Z-F(倪中福), Liu W-Q(刘旺清), Sun Q-X(孙其信). Comparative analysis of genetic diversity revealed by genomic-SSR, EST-SSR and pedigree in wheat (Triticum asetivum L.). Acta Genet Sin (遗传学报), 2005, 32(4): 406–416 (in Chinese with English abstract)

[8]Kantety R V, La Rota M, Matthews D E, Sorrells M E. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Mol Biol, 2002, 501–510

[9]Cordeiro G M, Casu R, McIntyre C L, Manners J M, Henry R J. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum. Plant Sci, 2001, 160(6): 1115–1123

[10]Varshney R K, Thiel T, Stein N, Langridge P, Graner A. In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal species. Cell Mol Biol Lett, 2002, 537–546

[11]Cho Y G, Ishii T, Temnykh S, Chen X, Lipovich L, McCouch S R, Park W D, Ayres N, Cartinhour S. Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). Theor Appl Genet, 2000, 100: 713–722

[12]Chang W(常玮), Zhao X(赵雪), Li X(李侠), Qiu B(邱波), Han Y-P(韩英鹏), Teng W-L(縢伟丽), Li W-B(李文滨). Development of soybean EST-SSR marker and comparison with genomic-SSR marker. Chin J Oil Crop Sci (中国油料作物学报), 2009, 31(2): 149–156 (in Chinese with English abstract)

[13]Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucl Acids Res, 1999, 27: 573–580

[14]Castelo A T, Martins W, Gao G R. TROLL-tandem repeat occurrence locator. Bioinformatics, 2002, 18: 634–636

[15]Saha M C, Mian M A, Eujayl I, Z wonitzer J C, Wang L, May G D. Tall fescue EST-SSR markers with transferability across several grass species. Theor Appl Genet, 2004, 109: 783–791

[16]Hu J-B(胡建斌), Li J-W(李建吾). Distribution of EST-SSRs in cucumber genome and development of EST-SSR marker. Acta Bot Borea-Occident Sin (西北植物学报), 2008, 28(12): 2429–2435 (in Chinese with English abstract)

[17]Liang X Q, Chen X P, Hong Y B, Liu H Y, Zhou G Y, Li S X, Guo B Z. Utility of EST-derived SSR in cultivated peanut(Arachis hypogaea L.) and Arachis wild species. BMC Plant Biol, 2009, 9: 35

[18]Liang X-Q(梁炫强), Hong Y-B(洪彦彬), Chen X-P(陈小平), Liu H-Y(刘海燕), Zhou G-Y(周桂元), Li S-X(李少雄), Wen S-J(温世杰). Characterization and application of EST-SSRs in peanut (Arachis hypogaea L.). Acta Agron Sin (作物学报), 2009, 35(2): 246−254 (in Chinese with English abstract)

[19]Tang R-H(唐荣华), He L-Q(贺梁琼), Zhuang W-J(庄伟建), Han Z-Q(韩柱强), Zhong R-C(钟瑞春), Zhou C-Q(周翠球), Gao G-Q(高国庆), Li Z(李忠). Phylogenetic relation ships in the genus Arachis based on SSR molecular marker profiles. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(2): 36–41 (in Chinese with English abstract)

[20]Rohlf F J. NTSYSpc: Numerical Taxonomy and Multivariate Analysis System Version 2.1. Exeter Software, 2000

[21]Proite K, Leal-Bertioli S C, Bertioli D J, Moretzsohn M C, da Silva F R, Martins N F, Guimaraes P M: ESTs from a wild Arachis species for gene discovery and marker development. BMC Plant Biol, 2007, 7: 7

[22]Chen B-Y(陈本银), Jiang H-F(姜慧芳), Liao B-S(廖伯寿), Ren X-P(任小平), Huang J-Q(黄家权), Lei Y(雷永), Wang S-Y(王圣玉). Genetic diversity analysis of Arachis gerplasm by SSR. J Trop Subtrop Bot (热带亚热带植物学报), 2008, 16(4): 296–303 (in Chinese with English abstract)

[23]Khedikar Y P, Gowda M V C, Sarvamangala C, Patgar K V, Upadhyaya H D, Varshney R K. A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resisitance in groundnut (Arachis hypogaea L.). Theor Appl Genet, 2010, 121: 971−984

[24]Ren X-P(任小平), Jiang H-F(姜慧芳), Liao B-S(廖伯寿). Identification of molecular markers for resistance to bacterial wilt in peanut (Arachis hypogeea L.). J Plant Genet Resour (植物遗传资源学报), 2008, 9(2): 163–167 (in Chinese with English abstract)

[25]Chen S-L(陈四龙), Li Y-R(李玉荣), Cheng Z-S(程增书), LiaoB-S(廖伯寿), Lei Y(雷永), Liu J-S(刘吉生). Heterosis and genetic analysis of oil content in peanut using mixed model of major gene and polygene. Sci Agric Sin (中国农业科学), 2009, 42(9): 3048−3057 (in Chinese with English abstract)

[26]Varshney R K, Chabane K, Hendre P S, Aggarwal R K, Graner A. Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Sci, 2007, 173(6): 638–649

[27]Zhao J Y, Becker H C, Zhang D Q, Zhang Y F, Ecke W. Oil content in a European×Chinese rapeseed population: QTL with additive and epistatic effects and their genotype−environment interactions. Crop Sci, 2005, 45: 51−59

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源于栽培种花生的EST-SSR引物对野生花生扩增的多态性

Genetic Diversity of Arachis Accessions Detected by EST-SSR from Cultivated Peanut (Arachis hypogaea L.)

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