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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1625-1630.doi: 10.3724/SP.J.1006.2012.01625

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

灰飞虱胁迫下水稻防卫相关基因的表达

李万昌1,2,**，余娇娇1,2,**，段灿星1,*，朱振东1，王晓鸣1   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程，北京100081；2 河南师范大学，河南新乡453007
  • 收稿日期:2012-02-09 修回日期:2012-04-20 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 段灿星, E-mail: duancx@caas.net.cn, Tel: 010-82109609? ** 同等贡献(Contributed equally to this work)
  • 基金资助:

    本研究由国家自然科学基金项目(30971746)和国家转基因生物新品种培育科技重大专项(2009ZX08009-046B)资助。

Expression of Rice Defence Genes under Small Brown Planthopper Stress

LI Wan-Chang1,2,**,YU Jiao-Jiao1,2,**,DUAN Can-Xing1,*,ZHU Zhen-Dong1,WANG Xiao-Ming1   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Genetic Resources and Improvement, Beijing 10081, China; 2 Henan Normal University, Xinxiang 453007, China
  • Received:2012-02-09 Revised:2012-04-20 Published:2012-09-12 Published online:2012-07-03
  • Contact: 段灿星, E-mail: duancx@caas.net.cn, Tel: 010-82109609? ** 同等贡献(Contributed equally to this work)

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摘要： 灰飞虱是我国水稻生产上的一种重要害虫。运用荧光定量PCR方法及特异性引物，对不同时间(12、24、36、48和72 h)灰飞虱胁迫下抗虫和感虫水稻品种中主要防卫途径的相关基因进行转录水平上定量分析。灰飞虱取食后，与水杨酸合成途径相关的基因PAL、NPR1、EDS1和PAD4在抗灰飞虱品种Mudgo中的表达水平均高于在感虫水稻Kittake中。接虫12 h后，PAL基因表达量达到未接虫时的6.914倍；在Mudgo中，PAL基因相对表达量上升更快，在24、48和72 h分别是Kittake中的42.848、70.743和69.193倍。NPR1基因在灰飞虱为害12、36和72 h后，在Mudgo中的表达量分别是Kittake中的4.690、6.231和4.112倍。与茉莉酸合成相关的基因LOX和AOS2，在灰飞虱为害36 h后，在Kittake中的表达水平显著高于Mudgo中。乙烯信号途径中的受体基因EIN2在Kittake中的表达量也高于Mudgo中。结果表明，灰飞虱取食激活了抗虫水稻Mudgo中依赖水杨酸介导的抗性途径，同时诱导感虫水稻Kittake产生了依赖茉莉酸/乙烯途径的防卫反应，PAL和NPR1基因的表达在调节Mudgo抗灰飞虱中具有重要作用。

关键词: 水稻, 灰飞虱, 防卫基因, 荧光定量PCR

Abstract: The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacide), is an economically important pest in rice (Oryza sativaL.) production in China.Real-time PCR was used to determine transcriptional level of rice defence genes after SBPH infestation using specific primers. The expression level of SA synthesis-related genes PAL, NPR1, EDS1, and PAD4 was higher in resistant Mudgo than in susceptible Kittake after SBPHfeeding. The expression level of gene PAL in 12 h-infestation rice was 6.914 times of that in untreated rice. The gene PAL accumulation was more rapid and at higher levels in Mudgo and the expression amount in Mudgo was 42.848, 70.743, and 69.193 times of that in Kittake at 24, 48, and 72 h after SBPH infestation, respectively.The gene NPR1 expression in Mudgo was 4.690, 6.231, and 4.112 times of that in Kittake after SBPH infestation for 12, 36, and 72 h. There was significant difference in transcriptional level of the JA synthesis-related genes LOX and AOS2 after 36 h-infestation between Mudgo and Kittake. The expression level was substantially lower in Mudgo than in Kittake at subsequent time points. In addition, the expression level of receptor gene EIN2 in ethylene signaling pathway was higher in Kittake than in Mudgo after SBPHfeeding. The above results indicated that SBPH feeding activatedthe salicylic acid signaling pathway in resistantMudgo and induced the defenses in susceptible Kittake associated with a JA/ethylene-dependent pathway. The genesPAL and NPR1 played a considerable role in the regulation of Mudgo expressing resistanceto SBPH.

Key words: Rice, Small brown Planthopper, Defence genes, Real-time PCR

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