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作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1350-1355.doi: 10.3724/SP.J.1006.2014.01350

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

赤霉素生物合成酶基因GhCPSGhKS参与甲哌鎓对棉花幼苗叶片生长的控制

王丽1,2,张明才1,杜明伟1,田晓莉1,李召虎1   

  1. 1 植物生长调节剂教育部工程研究中心 / 中国农业大学农学与生物技术学院, 北京100193; 2 河南师范大学生命科学学院, 河南新乡453007
  • 收稿日期:2014-03-06 修回日期:2014-04-16 出版日期:2014-08-12 网络出版日期:2014-06-03
  • 通讯作者: 李召虎, E-mail: lizhaohu@cau.edu.cn, Tel: 010-62733427
  • 基金资助:

    本研究由国家自然科学基金项目(31271628)资助。

GhCPS and GhKS Encoding Gibberellin Biosynthesis Enzymes Involve in Inhibition of Leaf Growth by Mepiquat Chloride in Cotton (Gossypium hirsutum L.)

WANG Li1,2,ZHANG Ming-Cai1,DU Ming-Wei1,TIAN Xiao-Li1,LI Zhao-Hu1,*   

  1. 1 Engineering Research Center of Plant Growth Regulator, Ministry of Education / College of Agronomy and Biotechnology, China Agricultural University, Beijing 10093, China; 2 College of Life Science, Henan Normal University, Xinxiang 453007, China
  • Received:2014-03-06 Revised:2014-04-16 Published:2014-08-12 Published online:2014-06-03
  • Contact: 李召虎, E-mail: lizhaohu@cau.edu.cn, Tel: 010-62733427

摘要:

室内盆栽欣抗4,在棉花幼苗第3片真叶完全展平时(4叶未展开)叶面喷施甲哌鎓(DPC),研究DPC对棉花幼苗叶片生长的控制与赤霉素(GA)合成早期关键酶柯巴基焦磷酸合酶(CPS)和内根-贝壳杉烯合酶(KS)基因表达的关系。结果表明,DPC处理显著减小棉花幼苗第34叶的叶面积,第4叶叶面积受控制程度较第3叶大;80 mg L–1DPC处理的棉花幼苗第34叶中GA4含量分别于处理后4 d4~6 d显著低于对照;与对照相比,80 mg L–1 DPC处理的棉花幼苗第3叶中GhCPSGhKS表达在处理后1~4 d显著降低,而第4叶中GhCPSGhKS的表达在处理后1~6 d显著降低。由此可见,DPC通过影响GhCPSGhKS的表达,降低内源活性GA4的含量,控制棉花幼苗叶片生长,且较幼嫩叶片对DPC较敏感。

关键词: 棉花, 甲哌鎓, 柯巴基焦磷酸合酶, 内根-贝壳杉烯合酶, 叶面积

Abstract:

Ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) are the key enzymes involved in the early steps of gibberellin (GA) biosynthesis. This paper aimed at elucidating whether the action of mepiquat chloride (DPC) on leaf growth was related to the expression levels of GhCPS and GhKS in cotton seedlings. DPC was foliar applied to seedlings at the 3rd leaf expanded stage of cotton cultivar Xinkang 4 by pot culture. The results showed that DPC significantly decreased the leaf area, and the area of the 4th leaf was decreased more than that of the 3rd leaf. DPC at 80 mg L–1 markedly reduced GA4 content in the 3rd leaf at four days after treatment and in the 4th leaf from four to six days after treatment. The expression levels of GhCPS and GhKS in the 3rd leaf were decreased by DPC from one to four days after treatment, and similar trends were observed in the 4th leaf from one to six days after treatment. All the results suggested that DPC could reduce endogenous GA4 content by downregulating GhCPS and GhKS expressions, leading to a smaller leaf size. Otherwise, the younger leaf was more sensitive to DPC.

Key words: Cotton, Mepiquat chloride, Ent-copalyl diphosphate synthase, Ent-kaurene synthase;Leaf area

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