作物学报 ›› 2015, Vol. 41 ›› Issue (02): 297-307.doi: 10.3724/SP.J.1006.2015.00297
徐云姬,顾道健,秦昊,张耗,王志琴,杨建昌*
XU Yun-Ji,GU Dao-Jian,QING Hao,ZHANG Hao,WANG Zhi-Qin,YANG Jian-Chang*
摘要:
玉米果穗顶部籽粒通常较中、下部籽粒充实差,粒重轻,其机制不清楚。本研究旨在探明玉米果穗不同部位籽粒淀粉合成相关酶活性变化及其与籽粒灌浆的关系。以玉米品种登海11为材料,分别进行春播和夏播试验,观察果穗不同部位籽粒中可溶性糖、蔗糖和淀粉的含量及淀粉合成相关酶活性变化。结果显示,与夏播玉米相比,春播玉米具有较多的每穗粒数、较高的百粒重和产量。虽然产量在春播和夏播间有差异,但两季玉米籽粒的最大灌浆速率、平均灌浆速率、百粒重、可溶性糖和蔗糖含量、最大淀粉积累速率、平均淀粉积累速率均表现为果穗下部籽粒>中部籽粒>上部籽粒。灌浆期果穗不同部位籽粒腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)、淀粉合酶(StS)和淀粉分支酶(SBE)活性变化均呈单峰曲线,果穗上部籽粒AGPase、StS和SBE活性峰值和平均值均显著低于果穗中、下部籽粒。相关分析表明,淀粉积累速率、籽粒灌浆速率与AGPase、StS和SBE活性均呈极显著正相关。说明玉米果穗顶部籽粒较低的AGPase、StS 和SBE活性是其灌浆较差、粒重较低的重要原因。春播玉米粒重较高,与其灌浆期较强的淀粉合成能力有关。
| [1]徐云姬, 顾道健, 张博博, 张耗, 王志琴, 杨建昌. 玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系. 作物学报, 2013, 39: 1452–1461Xu Y J, Gu D J, Zhang B B, Zhang H, Wang Z Q, Yang J C. Hormone contents in kernels at different positions on an ear and their relationship with endosperm development and kernel filling in maize. Acta Agron Sin, 2013, 39: 1452–1461 (in Chinese with English abstract)[2]Sikder H P, Gupta D K D. Physiology of grain in rice. Indian Agric, 1976, 20: 133–141[3]Wang Y. Effectiveness of supplied nitrogen at the primordial panicle stage on rice characteristics and yields. Int Rice Res Newsl, 1981, 6: 23–24[4]Murty P S S, Murty K S. Spikelet sterility in relation to nitrogen and carbohydrate contents in rice. Indian J Plant Physiol, 1982, 25: 40–48[5]杨卫兵, 王振林, 尹燕枰, 李文阳, 李勇, 陈晓光, 王平, 陈二影, 郭俊祥, 蔡铁, 倪英丽. 外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响. 中国农业科学, 2011, 44: 2673–2682Yang W B, Wang Z L, Yin Y P, Li W Y, Li Y, Chen X G, Wang P, Chen E Y, Guo J X, Cai T, Ni Y L. Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin, 2011, 44: 2673–2682 (in Chinese with English abstract)[6]黄升谋, 邹应斌. 玉米素和吲哚乙酸与强弱势粒灌浆及胚乳细胞形成. 农业现代化研究, 2004, 25(6): 470–473Huang S M, Zou Y B. Relationship of zeatin and indo-acetic acid with grains-filling and development of endosperm cells in superior and inferior grains. Res Agric Modern, 2004, 25(6): 470–473 (in Chinese with English abstract)[7]Jin D M, Wang W J, Lan S Y. Dynamic status of endogenous IAA, ABA and GA levels in superior and inferior spikelets of heavy panicle hybrid rice during grain filling. J Plant Physiol Mol Biol, 2002, 28: 215–220[8]Yang J, Peng S, Visperas R M, Sanico A L, Zhu Q, Gu S. Grain filling pattern and cytokinin content in the grains and roots of rice plants. Plant Growth Regul, 2000, 30: 261–270[9]王艳芳, 崔震海, 阮燕晔, 马兴林, 关义新, 张立军. 不同类型春玉米灌浆期间籽粒中内源激素IAA、GA、ZR、ABA含量的变化. 植物生理学通讯, 2006, 42: 225–228Wang Y F, Cui Z H, Ruan Y Y, Ma X L, Guan Y X, Zhang L J. Changes in endogenous hormone of IAA, GA, ZR and ABA in kernels during grain-filling stage in different types of spring maize (Zea mays L.). Plant Physiol Commun, 2006, 42: 225–228 (in Chinese with English abstract)[10]王纪华, 王树安, 赵冬梅, 夏厚军. 玉米穗轴维管解剖结构及含水率对籽粒发育的影响. 玉米科学, 1994, 2: 41–43Wang J H, Wang S A, Zhao D M, Xia H J. Study on the effects of cob vascular structure and water ratio on kernel development. J Maize Sci, 1994, 2: 41–43 (in Chinese with English abstract)[11]付景, 徐云姬, 陈露, 袁莉民, 王志琴, 杨建昌.超级稻花后强、弱势粒淀粉合成相关酶活性和激素含量变化及其与籽粒灌浆的关系. 作物学报, 2012, 26: 302–310Fu J, Xu Y J, Chen L, Yuan L M, Wang Z Q, Yang J C. Post-anthesis changes in activities of the enzymes related to starch synthesis and contents of hormones in superior and inferior spikelets and their relation with grain filling of super rice. Acta Agron Sin, 2012, 26: 302–310 (in Chinese with English abstract)[12]Yang J, Zhang J, Wang Z, Xu G, Zhu Q. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected: to water deficit during grain filling. Plant Physiol, 2004, 135: 1621–1629[13]Yang J, Zhang J, Wang Z, Zhu Q, Liu L. Activities of enzymes involved in source-to-starch metabolism in rice grains subjected to water stress during filling. Field Crops Res, 2003, 81: 69–81[14]陈婷婷, 谈桂露, 褚光, 刘立军, 杨建昌. 超级稻花后强、弱势粒灌浆相关蛋白质表达的差异. 作物学报, 2012, 38: 1471–1482Chen T T, Tan G L, Chu G, Liu L J, Yang J C. Differential expressions of the proteins related to grain filling between superior and inferior spikelets of super rice after anthesis. Acta Agron Sin, 2012, 38: 1471–1482 (in Chinese with English abstract)[15]Ishimaru T, Hirose T, Matsuda T, Goto A, Takahashi K, Sasaki H, Terao T, Ishii R, Ohsugi R, Yamagishi T. Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle. Plant Cell Physiol, 2005, 46: 620–628[16]黄艳胜. 玉米籽粒发育及产量形成. 中国林副特产, 2002, (1): 33Wang Y S. Kernel development in maize and yield formation. Quarterly of Forest by-Product and Specialty in China, 2002, (1): 33 (in Chinese)[17]王忠. 植物生理学. 北京: 中国农业出版社, 2000Wang Z. Plant Physiology. Beijing: China Agriculture Press, 2000 (in Chinese) [18]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol, 1989, 30: 833–839[19]Nakamura Y, Yuki K. Changes in enzyme activities associated with carbohydrate metabolism during development of rice endosperm. Plant Sci, 1992, 82: 15–20[20]贺明荣, 王振林, 张杰昌. 小麦开花后光合物质在不同穗位间的分配及其与穗粒重的关系. 作物学报, 2000, 26: 190–194He M R, Wang Z L, Zhang J C. Distribution of photoassimilate to different parts of wheat ear after anthesis and its relation to kernel weight per ear. Acta Agron Sin, 2000, 26: 190–194 (in Chinese with English abstract)[21]张凤路, 江亚丽, 赵国顺, 张俊花. 14C-同化物在玉米果穗上的分布与籽粒败育关系. 作物学报, 2006, 32: 1104–1106Zhang F L, Jiang Y L, Zhao G S, Zhang J H. Relationship between distribution of 14C-assimilates and kernel abortion in maize. Acta Agron Sin, 2006, 32: 1104–1106 (in Chinese with English abstract)[22]张丽华, 李海军, 王艳哲, 李金才, 崔彦宏, 马瑞崑. 玉米花丝和穗轴中可溶性糖、淀粉变化规律的研究. 玉米科学, 2005, 13: 64–67Zhang L H, Li H J, Wang Y Z, Li J C, Cui Y H, Ma R K. Study on the dynamic of soluble sugar and starch in the silk and cob of corn. J Maize Sci, 2005, 13: 64–67(in Chinese with English abstract)[23]刘海龙, 何萍, 金继运, 李文娟, 张宽, 王秀芳, 谢佳贵, 尹彩侠, 侯云鹏. 施氮对高淀粉玉米和普通玉米子粒可溶性糖和淀粉积累的影响. 植物营养与肥料学报, 2009, 15: 493–500Liu H L, He P, Jin J Y, Li W J, Zhang K, Wang X F, Xie J G, Yin C X, Hou Y P. Effects of nitrogen nutrition on sugar and starch accumulation of high starch maize and common maize. Plant Nutr Fert Sci, 2009, 15: 493–500 (in Chinese with English abstract)[24]王艳芳, 杨东伟, 张立军, 马兴林, 关义新. 高油玉米灌浆期籽粒可溶性糖含量与脂肪积累的关系. 沈阳农业大学学报, 2006, 37: 144–146Wang Y F, Yang D W, Zhang L J, Ma X L, Guan Y X. Relationship between fat accumulation and soluble sugar contents in kernels during grain-filling stage in maize. J Shenyang Agric Univ, 2006, 37: 144–146 (in Chinese with English abstract)[25]张海燕, 董树亭, 高荣岐, 李玉全. 玉米籽粒淀粉积累及相关酶活性分析. 中国农业科学, 2008, 41: 2174–2181Zhang H Y, Dong S T, Gao R Q, Li Y Q. Starch accumulation and enzymes activities associated with starch synthesis in maize kernels. Sci Agric Sin, 2008, 41: 2174–2181 (in Chinese with English abstract)[26]Zhang J J, Hu Y F, Zhou H, Huang Y B. Starch accumulation and activities of key enzymes involved in starch synthesis in the grains of maize inbred lines with different starch contents. J Plant Physiol Mol Biol, 2007, 33: 123–130[27]赵步洪, 张文杰, 常二华, 王志琴, 杨建昌. 水稻灌浆期籽粒中淀粉合成关键酶的活性变化及其与灌浆速率和蒸煮品质的关系. 中国农业科学, 2004, 37: 1123–1129Zhao B H, Zhang W J, Chang E H, Wang Z Q, Yang J C. Changes in activities of the key enzymes related to starch synthesis in rice grains during grain filling and their relationships with the filling rate and cooking quality. Sci Agric Sin, 2004, 37: 1123–1129 (in Chinese with English abstract)[28]王志琴, 叶玉秀, 杨建昌, 袁莉民, 王学明, 朱庆森. 水稻灌浆期籽粒中蔗糖合成酶活性的变化与调节. 作物学报, 2004, 30: 634–643Wang Z Q, Ye Y X, Yang J C, Yuan L M, Wang X M, Zhu Q S. Changes and regulations of sucrose synthase activity in rice grains during grain filling. Acta Agron Sin, 2004, 30: 634–643 (in Chinese with English abstract)[29]Ahmadi A, Baker D A. The effect of water stress on the activities of key regulatory enzymes of the sucrose to starch pathway in wheat. Plant Growth Regul, 2001, 35: 81–91[30]Kato T. Change of sucrose synthase activity in developing endosperm of rice cultivars. Crop Sci, 1995, 35: 827–831[31]梁建生, 曹显祖, 徐生, 朱庆森, 宋平. 水稻籽粒库强与其淀粉积累之间关系的研究. 作物学报, 1994, 20: 685–691Liang J S, Cao X Z, Xu S, Zhu Q S, Song P. Studies on the relationship between the grain sink strength and its starch accumulation in rice (O. sativa). Acta Agron Sin, 1994, 20: 685–691 (in Chinese with English abstract)[32]Doehlert D C. Distribution of enzyme activities within the developing maize (Zea mays) kernel in relation to starch, oil and protein accumulation. Physiol Plant, 1990, 78: 560–567[33]Singletary G W, Banisadr R, Keeling P L. Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize. Plant Physiol, 1997, 113: 293–304[34]Cao H, Imparl-Radosevich J, Guan H, Keeling P L, James M G, Myers A M. Identification of the soluble starch synthase activities of maize endosperm. Plant Physiol, 1999, 120: 205–215[35]朱庆森, 曹显祖, 骆亦其. 水稻籽粒灌浆的生长分析. 作物学报, 1988, 14: 182–193Zhu Q S, Cao X Z, Luo Y Q. Growth analysis on the process of grain filling in rice. Acta Agron Sin, 1988, 14: 182–193 (in Chinese with English abstract)[36]Richards F J. A flexible growth function for empirical use. J Exp Bot, 1959, 10: 290–300[37]张宪政, 陈凤玉, 王荣富. 植物生理学实验技术. 沈阳: 辽宁科学技术出版社, 1994. pp 144–151Zhang X Z, Chen F Y, Wang R F. Experiment Technology of Plant Physiology. Shenyang: Liaoning Science and Technology Press, 1994. pp 144–151 (in Chinese)[38]何照范. 粮油籽粒品质及其分析技术. 北京: 中国农业出版社,1985. pp 144–150He Z F. Grain Quality of Cereals for Gaining Oil and Its Analysis Technique. Beijing: China Agriculture Press, 1985. pp 144–150 (in Chinese)[39]程方民, 蒋德安, 吴平, 石春海. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征. 作物学报, 2001, 27: 201–206Cheng F M, Jiang D A, Wu P, Shi C H. The dynamic change of starch synthesis enzymes during the grain filling stage and effects of temperature upon it. Acta Agron Sin, 2001, 27: 201–206 (in Chinese with English abstract)[40]李太贵, 沈波, 陈能, 罗玉坤. Q酶在水稻籽粒垩白形成中作用的研究. 作物学报, 1997, 23: 338–344Li T G, Shen B, Chen N, Luo Y K. Effect of Q-enzyme on the chalkiness formation of rice grain. Acta Agron Sin, 1997, 23: 338–344 (in Chinese with English abstract)[41]Yang J C, Zhang J H, Wang Z Q, Liu K, Wang P. Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene. J Exp Bot, 2006, 57: 149–160[42]王芳, 王宪泽. 小麦籽粒淀粉合成动态及其相关酶活性的研究. 麦类作物学报, 2004, 24: 57–60Wang F, Wang X Z. Study on the dynamic changes of starch synthesis and their related enzymes in wheat. J Triticeae Crops, 2004, 24: 57–60 (in Chinese with English abstract)[43]Ou-Lee T M, Setter T L. Effect of increased temperature in apical regions of maize ears on starch-synthesis enzymes and accumulation of sugars and starch. Plant Physiol, 1985, 79: 852–855[44]Jiang D, Cao W, Dai T, Jing Q. Activities of key enzymes for starch synthesis in relation to growth of superior and inferior grains on winter wheat (Triticum aestivum L.) spike. Plant Growth Regul, 2003, 41: 247–257[45]申丽霞, 王璞, 张红芳, 易镇邪. 施氮对夏玉米不同部位籽粒灌浆的影响. 作物学报, 2005, 31: 532–534Shen L X, Wang P, Zhang H F, Yi Z X. Effect of nitrogen supply on grain filling at different ear position in summer maize. Acta Agron Sin, 2005, 31: 532–534 (in Chinese with English abstract)[46]王元明. 玉米穗中部籽粒作种研究的初步报告.中国农业科学, 1952, 4: 29Wang Y M. Preliminary report on the research for middle kernels as seeds in maize corn. Sci Agric Sin, 1952, 4: 29 (in Chinese)[47]Zhang H, Li H W, Yuan L M, Wang Z Q, Yang J C, Zhang J H. Post-anthesis alternate wetting and moderate soil drying enhances activities of key enzymes in sucrose-to-starch conversion in inferior spikelets of rice. J Exp Bot, 2012, 63: 215–227[48]肖长新, 陈延玲, 米国华. 灌浆后期6-BA灌根对玉米衰老和产量形成的影响. 玉米科学, 2014, 22(1): 103–107, 113Xiao C X, Chen Y L, Mi G H. Effect of treating root with 6-BA at late grain filling stage on leaf senescence and yield of maize. J Maize Sci, 2014, 22(1): 103–107, 113 (in Chinese with English abstract)[49]杨升辉, 杨恒山, 李洪杰, 唐汝友, 王素阁, 徐长帅, 陈勇. 不同氮肥运筹下春玉米子粒灌浆特性的分析. 玉米科学, 2014, 22(1): 91–95Yang S H, Yang H S, Li H J, Tang R Y, Wang S G, Xu C S, Chen Y. Analysis on kernel filling characteristics for spring maize in different nitrogen fertilizer strategies. J Maize Sci, 2014, 22(1): 91–95 (in Chinese with English abstract) |
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