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作物学报 ›› 2015, Vol. 41 ›› Issue (06): 956-962.doi: 10.3724/SP.J.1006.2015.00956

• 耕作栽培·生理生化 • 上一篇    下一篇

外源水杨酸对Cu胁迫下水培烟草生长及营养元素吸收利用的影响

徐根娣1,2,葛淑芳1,2,章艺3,吴玉环4,梅笑漫5,刘鹏1,2,*   

  1. 1 浙江师范大学生态学研究所, 浙江金华 321004；2 浙江师范大学植物学实验室, 浙江金华 321004；3 浙江旅游职业学院, 浙江杭州 3112313；4 杭州师范大学生命与环境科学学院, 浙江杭州 330036；5 杭州师范大学初等教育学院，浙江杭州 330036
  • 收稿日期:2014-10-27 修回日期:2015-04-02 出版日期:2015-06-12 网络出版日期:2015-04-14
  • 基金资助:

    本研究由国家自然科学基金项目(30970188, 30540056)和浙江省公益技术研究农业项目(2011C22053)。

Effect of Salicylic Acid on Growth and Nutrient Uptake in Hydroponic Tobacco under Cu Stress

XU Gen-Di1,2,GE Shu-Fang1,2,ZAHNG Yi3,WU Yu-Huang4,MEI Xiao-Man5,LIU Peng1,2,*   

  1. 1 Research Institute of Ecology, Zhejiang Normal University, Jinhua 321004, China; 2 Key Laboratory of Botany / Zhejiang Normal University, Jinhua 321004, China; 3 Tourism College of Zhejiang, Hanzhou 311231, China; 4 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; 5 College of Biological and Environmental Sciencess, Hanzhou Normal University, Hangzhou 330036, China
  • Received:2014-10-27 Revised:2015-04-02 Published:2015-06-12 Published online:2015-04-14

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摘要：

以耐Cu性较强的烟草品种W38 (Nicotiana tabacum cv. W38)和耐Cu性较弱的本氏烟(Nicotiana benthamiana)为试验材料，设置水培处理组即CK (Cu2+ 0 mg L–1，SA 0 μmol L–1，即对照组)、T1(Cu2+ 4 mg L–1，SA 0 μmol L–1)、T2 (Cu2+ 4 mg L–1，SA 100 μmol L–1)、T3 (Cu2+ 4 mg L–1，SA 300 μmol L–1)、T4 (Cu2+ 4 mg L–1，SA 500 μmol L–1)，探讨SA对烟草生长特性及根、茎、叶不同器官中元素吸收规律的影响。结果表明，经过4 mg L–1 Cu胁迫15 d后，2个品种的烟草生长均受到一定程度的抑制，不同器官中Cu含量均显著升高，K、Ca、Mg、Fe、Zn、B、Mn的吸收受到抑制，而在营养液中添加适宜浓度的SA能够有效缓解Cu胁迫对2个品种烟草根长、株高、鲜重的抑制作用，并降低烟草体内Cu含量。与T1处理组相比，本氏烟的根、茎、叶中Cu含量最大分别下降25.05%、39.78%和22.91%，W38的根、茎、叶中的Cu含量最大分别下降23.27%、37.30%和28.88%，并促进了营养元素K、Ca、Mg、Fe、Mn的吸收，但是对Zn、B元素的吸收影响并不明显。由此可知，高浓度的Cu胁迫会抑制烟草的生长及营养元素的吸收和运输，施加适宜浓度的外源水杨酸有利于营养元素的吸收和促进烟草的生长发育，在本试验浓度范围内施加300 μmol L–1 的水杨酸可显著缓解Cu胁迫对烟草的抑制作用。

关键词: SA, Cu胁迫, 烟草, 微量元素

Abstract:

A hydroponic experiment was conducted to study the effects from treatmeats including CK ( Cu2+ 0 mg L–1, SA 0 μmol L–1 as control), T1 (Cu2+ 4 mg L–1, SA 0 μmol L–1), T2 (Cu2+ 4 mg L–1, SA 100 μmol L-1), T3 (Cu2+ 4 mg L–1, SA 300 μmol L–1), T4 (Cu2+ 4 mg L–1, SA 500 μmol L–1)on growth characteristics, elements absorption and accumulation in two tobacco cultivars, N. tabacum cv. W38 (Cu resistant genotype) and N. benthamiana (Cu sensitive genotype) under copper stress. The result showed that the growth of two tobacco cultivars under 4 mg L–1 Cu stress for 15 days was inhibited to some extent, meanwhile, the content of Cu in their organs was increased while the absorptions of K, Ca, Mg, Fe, Zn, B, and Mn were inhibited. The addition of exogenous SA could alleviate the inhibition of plant height, root length and fresh weight, and reduce Cu content of tobacco, but increase the absorption of nutritional elements K, Ca, Mg, Fe, and Mn. Compared with treatment T1, contents of Cu in root, stem and leaf were increased by 25.05%, 39.78%, 22.91% in N. benthamiana, and by 23.27%, 37.30%, 28.88% in N. tabacum cv.W38, respectively. In conclusion, appropriate concentration of SA promotes the growth and elements absorption in tobacco under copper stress. In this experiment, 300 μmol L–1SA could significantly alleviate copper inhibition to tobacco.

Key words: Salicylic acid, Cu stress, Tobacco, Trace elements

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