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作物学报 ›› 2019, Vol. 45 ›› Issue (9): 1319-1326.doi: 10.3724/SP.J.1006.2019.82062

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于通路分析剖析水稻农艺性状配合力和杂种优势

向丽媛1,徐凯1,苏静1,吴超1,袁雄1,郑兴飞2,刁英2,胡中立2,李兰芝1,*()   

  1. 1 湖南农业大学/湖南省农业大数据分析与决策工程技术研究中心, 湖南长沙410128
    2 武汉大学/杂交水稻国家重点实验室, 湖北武汉430072
  • 收稿日期:2018-12-13 接受日期:2019-05-12 出版日期:2019-09-12 网络出版日期:2019-05-31
  • 通讯作者: 李兰芝
  • 作者简介:E-mail: xly941026@163.com
  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0100101);湖南省自然科学基金项目(2016JJ3070);湖南农大作物种质创新与资源利用国家重点实验室培育基地开放课题资助(16KFXM03)

Genetic dissection of combining ability and heterosis of rice agronomic traits based on pathway analysis

XIANG Li-Yuan1,XU Kai1,SU Jing1,WU Chao1,YUAN Xiong1,ZHENG Xing-Fei2,DIAO Ying2,HU Zhong-Li2,LI Lan-Zhi1,*()   

  1. 1 Hunan Engineering & Technology Research Center for Agricultural Big Data Analysis & Decision-making/Hunan Agricultural University, Changsha 410128, Hunan, China;
    2 State Key Laboratory of Hybrid Rice/Wuhan University, Wuhan 430072, Hubei, China
  • Received:2018-12-13 Accepted:2019-05-12 Published:2019-09-12 Published online:2019-05-31
  • Contact: Lan-Zhi LI
  • Supported by:
    The study was supported by the National Key Research and Development Program(2016YFD0100101);the Natural Science Foundation of Hunan Province(2016JJ3070);the State Key Laboratory of Crop Germplasm Innovation and Resource Utilization Breeding Base(16KFXM03)

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摘要：

按照NCII遗传交配设计构建测交群体, 考察包括产量性状在内的9个农艺性状, 对水稻农艺性状表型、配合力和杂种优势进行通路分析, 以期为水稻品种的培育和改良提供理论基础。结果表明, 主穗实粒数与生物调控、千粒重与半胱氨酸和蛋氨酸代谢、主穗长与SNARE相关囊泡运动、主穗一/二次枝梗与依赖DNA的转录、株高与大分子代谢过程、主穗颖花数与花粉识别、有效穗数与水解酶活性、单株实粒重与嘌呤核苷结合等通路相关。为获得优良性状, 高配合力, 杂种优势可以从某一性状的相关通路进行研究, 找到调控该通路的相关基因, 以期为改良水稻一般配合力和获得杂种优势提供一定帮助。

关键词: 杂交水稻, GWAS, 通路分析, 配合力, 杂种优势

Abstract:

In order to provide theoretical foundation of cultivating and improving rice, under North Carolina design II, we constructed test cross population and conducted pathway analysis of phenotype performance, combining ability and heterosis of agronomic trait in present study. The experiments’ results reveal that the filled grains per panicle, 1000-grain weight, main panicle length, primary/secondary branch of main panicle, plant height, spikelet per panicle, tillers per plant, and yield per plant were separately related to pathway of biological regulation, cysteine and methionine metabolism, snare interactions in vesicular transport, DNA-dependent transcription, macromolecular metabolism, recognition of pollen, hydrolase activity, purine nucleoside binding and so on. Therefore, in order to obtain elite hybrids with high combining ability and heterosis, it is feasible to start from studying related pathways of a certain trait and related genes regulating the pathway.

Key words: hybrid rice, GWAS, pathway analysis, combining ability, heterosis

图1

4个数据集所含通路 V: 父本表型; GCA: 父本一般配合力; TC: 测交群体表型; BP: 测交群体的超亲优势值。"

表1

4个数据集共有的通路"

通路Pathway 类别Category 描述Description
CATALYTIC_ACTIVITY GO:0003824 催化活性Catalytic activity
CATION_BINDING GO:0043169 阳离子结合Cation binding
ION_BINDING GO:0043167 离子结合Ion binding
METABOLIC_PROCESS GO:0008152 代谢过程Metabolic process
PRIMARY_METABOLIC_PROCESS GO:0044238 初级代谢过程Primary metabolic process

图2

4个数据集9个性状通路结果 FGPP: 主穗实粒数; KGW: 千粒重; MPL: 主穗长; PBP: 主穗一次枝梗数; PH: 株高; SBP: 主穗二次枝梗数; SPP: 主穗颖花数; TP: 有效穗数; YD: 单株实粒重。"

表2

4个数据集不同性状个数所含的通路个数"

数据集
Dataset		 性状个数
Number of traits		 通路个数
Number of pathways
V 1 86
2 13
3 1
GCA 1 52
2 11
3 11
4 25
5 7
6 1
TC 1 51
2 20
3 32
4 4
5 7
7 1
8 3
BP 1 33
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