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作物学报 ›› 2019, Vol. 45 ›› Issue (8): 1137-1145.doi: 10.3724/SP.J.1006.2019.84159

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

甘蓝型油菜开花相关基因的鉴定及进化与表达分析

王艳花1,2,谢玲1,2,杨博1,2,曹艳茹1,2,李加纳1,2,*()   

  1. 1 西南大学农学与生物科技学院/油菜工程研究中心, 重庆 400715
    2 西南大学现代农业科学研究院, 重庆 400715
  • 收稿日期:2018-11-16 接受日期:2019-04-15 出版日期:2019-08-12 网络出版日期:2019-07-16
  • 通讯作者: 李加纳
  • 作者简介:E-mail: hawer313@163.com
  • 基金资助:
    本研究由重庆市民生工程主题专项项目(cstc2016shms-ztzx80020);高等学校学科创新引智基地项目(“111”项目)资助(B12006)

Flowering genes in oilseed rape: identification, characterization, evolutionary and expression analysis

WANG Yan-Hua1,2,XIE Ling1,2,YANG Bo1,2,CAO Yan-Ru1,2,LI Jia-Na1,2,*()   

  1. 1 College of Agronomy and Biotechnology, Southwest University/Chongqing Engineering Research Center for Rapeseed, Chongqing 400715, China
    2 Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
  • Received:2018-11-16 Accepted:2019-04-15 Published:2019-08-12 Published online:2019-07-16
  • Contact: Jia-Na LI
  • Supported by:
    This study was supported by the Special Project of Chongqing People’s Livelihood Project(cstc2016shms-ztzx80020);the Project of Intellectual Base for Discipline Innovation in Colleges and Universities (“111” Project)(B12006)

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摘要：

开花是开花植物繁衍后代的前提, 控制开花时间对农作物获得高产稳产具有重要影响。然而, 关于甘蓝型油菜基因组水平上开花相关基因的信息报道较少。本研究对甘蓝型油菜开花相关的基因进行了鉴定、特征分析、进化与表达模式分析。结果表明, 基因组水平上共鉴定到甘蓝型油菜1173个开花相关基因, 这些基因分为9类, 且不均匀地分布在染色体上; 与白菜(AA, 2n=20)和甘蓝(CC, 2n=18)相比, 甘蓝型油菜(AACC, 2n=38)经过自然杂交和染色体加倍后, 使开花相关基因的数目显著扩增; 选择压力分析表明, 糖代谢信号途径的基因比自发途径的开花相关基因受到的选择压力更大; 仍有一些开花相关基因在拟南芥和油菜基因组内非常保守。甘蓝型油菜基因数目的扩增和功能分化导致其开花调控机制更加复杂, 本研究为油菜开花途径提供更多的信息, 也为阐明拟南芥和油菜开花基因的进化关系指明了方向。

关键词: 甘蓝型油菜, 开花基因, 进化, 调控途径, 表达分析

Abstract:

Flowering is a prerequisite for successful sexual reproduction. Controlling of flowering time is important for crop production in different geographical regions. However, few information regarding flowering genes or their evolution at genome-wide level in Brassica napus has been reported. In this study, identification, characterization, evolutionary and expression analysis of flowering genes in oilseed rape were performed. In total, 1173 flowering-related genes classified into nine types and distributed unevenly on the chromosomes were identified at the genome level of Brassica napus. Compared with Brassica rapa (AA, 2n = 20) and Brassica oleraca (CC, 2n = 18), B. napus (AACC, 2n = 38) showed significantly enlarge number of flowering-related genes due to natural hybridization and chromosome doubling. Selective pressure analysis showed that the autonomous pathway genes had less selection pressure than the genes involved in sugar metabolic pathway, suggesting that some key flowering-related genes are relatively conserved between B. napus and Arabidopsis thaliana. The present study provides more information on the B. napus flowering pathways and sheds light on the evolutionary relationship of flowering-related genes between B. napus and A. thaliana.

Key words: Brassica napus, flowering genes, evolution, regulatory pathway, expression analysis

图1

甘蓝型油菜开花相关基因鉴定与分类 Ag: 衰老途径; At: 环境温度; Au: 自发途径; Cp: 光周期途径; Fd: 花器官发育及顶端分生组织响应途径; Ft: 开花时间调控者; Ho: 激素途径; Su: 糖信号; Ve: 春化。"

图2

甘蓝型油菜开花相关基因的染色体分布"

图3

不同开花途径基因在拟南芥、白菜、甘蓝和甘蓝型油菜基因组内的数目"

图4

甘蓝型油菜不同开花途径基因的选择压力分析"

图5

甘蓝型油菜开花相关基因的组织表达分析"

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