马铃薯资源晚疫病抗性的全基因组关联分析

doi:10.3724/SP.J.1006.2021.04099

作物学报 ›› 2021, Vol. 47 ›› Issue (2): 245-261.doi: 10.3724/SP.J.1006.2021.04099

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

马铃薯资源晚疫病抗性的全基因组关联分析

蒋伟¹^,²(), 潘哲超¹^,², 包丽仙¹^,², 周福仙², 李燕山¹^,², 隋启君¹^,²^,^*(), 李先平¹^,²^,^*()

¹云南省农业科学院经济作物研究所, 云南昆明 650205
²云贵高原马铃薯与油菜科学观测实验站, 云南昆明 650200

收稿日期:2020-04-30 接受日期:2020-09-13 出版日期:2021-02-12 网络出版日期:2020-09-28
通讯作者: 隋启君,李先平
作者简介:蒋伟, E-mail: jiangweiyaas@hotmail.com
基金资助:
国家自然科学基金项目(31760409);国家自然科学基金项目(31460368);云南省农业联合重点项目([2017FG001(-012)]);国际马铃薯中心合作项目TON(14.1432.5-001.00)

Genome-wide association analysis for late blight resistance of potato resources

JIANG Wei¹^,²(), PAN Zhe-Chao¹^,², BAO Li-Xian¹^,², ZHOU Fu-Xian², LI Yan-Shan¹^,², SUI Qi-Jun¹^,²^,^*(), LI Xian-Ping¹^,²^,^*()

¹Industrial Crops Research Institute, Yunnan Academy of Agricultural Science, Kunming 650205, Yunnan, China
²Scientific Observing and Experimental Station of Potato and Rapeseed in Yunnan-Guizhou Plateau, Kunming 650200, Yunnan, China

Received:2020-04-30 Accepted:2020-09-13 Published:2021-02-12 Published online:2020-09-28
Contact: SUI Qi-Jun,LI Xian-Ping
Supported by:
National Natural Science Foundation of China(31760409);National Natural Science Foundation of China(31460368);Yunnan Agricultural Foundation Projects([2017FG001(-012)]);International Potato Center Collaboration Program TON(14.1432.5-001.00)

摘要/Abstract

摘要：

广谱抗性基因的挖掘是马铃薯高抗晚疫病品种选育的基础。本研究以288份国际马铃薯中心筛选的晚疫病抗性群体为试验材料, 经过连续2年田间调查, 计算AUDPC和sAUDPC值, 评估群体晚疫病抗性; 利用SLAF-seq方法进行群体简化基因组测序, 通过对晚疫病抗性表型数据的全基因组关联分析, 挖掘晚疫病抗性相关的遗传位点和候选基因, 为晚疫病抗性品种选育和抗病机理研究提供一定的理论和材料基础。结果表明, 晚疫病抗性在288份材料间存在着广泛的遗传差异; 基于5种分析模型, 共鉴定到82个与晚疫病抗性显著关联的位点; 在关联区间关联到54个已知或可能与晚疫病抗性相关的基因。其中, 23个基因为抗性基因, 包括晚疫病抗性基因R1同源基因、Sw-5同源基因(R8)和Rpi-vnt1以及编码多效性耐药蛋白基因; 5个基因编码MAPK蛋白和WRKY转录因子; 1个基因参与茉莉酸途径; 3个基因与水杨酸途径相关; 6个基因是病程相关的基因; 3个基因参与苯基丙酸类合成途径; 其他与晚疫病抗性相关的基因, 如HMGR基因(2个)、细胞色素P450 (21个)。

关键词: 马铃薯, 晚疫病抗性, 全基因组关联分析, 抗性基因, 国际马铃薯中心

Abstract:

The mining of broad-spectrum resistance genes is the basis of the breeding of potato varieties with high late blight resistance. In this study, 288 samples of late disease resistance selected by the International Potato Center (CIP) for years were planted and were investigated for two consecutive years, which could provide some theoretical and material basis for breeding of late blight resistant varieties and help to study on disease resistance mechanism. The late blight resistance of each sample was evaluated using AUDPC and sAUDPC values. The population simplified genome sequencing was performed by SLAF-seq and carried out by genome-wide association analysis in order to discover the genetic loci and candidate genes related to late blight resistance. There were wide genetic differences in resistance to late blight among 288 samples. Through genome-wide association analysis, based on five analytical models, a total of 82 loci significantly correlated with late blight resistance were identified. Combined with genomic annotation, there were 54 candidate genes known or likely to be associated with late blight resistance in the association interval. Among them, 23 genes were resistant genes, including late blight resistance gene R1 homolog, Sw-5 homolog (R8) and Rpi-vnt1, as well as gene encoding pleiotropic resistance protein. Five genes encoded MAPK protein and WRKY transcription factor; one gene was involved in the JA pathway; three genes were associated with salicylic acid pathway; six genes were related to the course of the disease; three genes were involved in the synthesis of phenylpropionic acid. Other genes related to resistance to late blight included HMGR gene (2) and cytochrome P450 (21).

Key words: potato, late blight resistance, genome-wide association analysis, resistance gene, CIP

蒋伟, 潘哲超, 包丽仙, 周福仙, 李燕山, 隋启君, 李先平. 马铃薯资源晚疫病抗性的全基因组关联分析[J]. 作物学报, 2021, 47(2): 245-261.

JIANG Wei, PAN Zhe-Chao, BAO Li-Xian, ZHOU Fu-Xian, LI Yan-Shan, SUI Qi-Jun, LI Xian-Ping. Genome-wide association analysis for late blight resistance of potato resources[J]. Acta Agronomica Sinica, 2021, 47(2): 245-261.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2021.04099

https://zwxb.chinacrops.org/CN/Y2021/V47/I2/245

图/表 6

表1

自国际马铃薯中心引进的288份马铃薯群体材料"

编号 Code	CIP编号 CIP accession	群体 Population	编号 Code	CIP编号 CIP accession	群体 Population	编号 Code	CIP编号 CIP accession	群体 Population
C001	CIP381381.13	A	C106	CIP392740.4	LTVR	D095	CIP394900.1	BW
C002	CIP391065.69	B3	C107	CIP392745.7	LTVR	D096	CIP395011.2	B3
C003	CIP391583.25	B3	C108	CIP392759.1	LTVR	D097	CIP395015.6	B3
C004	CIP392617.54	B3	C109	CIP393613.2	LTVR	D098	CIP395017.14	B3
C005	CIP392634.52	B3	C110	CIP393615.6	LTVR	D099	CIP395017.227	B3
C006	CIP393073.179	B3	C112	CIP397030.31	LTVR	D100	CIP395017.229	B3
C007	CIP393073.197	B3	C113	CIP397035.26	LTVR	D101	CIP395017.242	B3
C008	CIP393227.66	B3	C114	CIP302428.20	LTVR	D102	CIP395037.107	B3
C009	CIP393228.67	B3	C115	CIP302476.108	LTVR	D103	CIP395077.12	B3
C010	CIP393371.164	B3	C116	CIP302499.30	LTVR	D105	CIP395096.2	B3
C011	CIP391004.18	B3	C117	CIP304345.102	LTVR	D106	CIP395109.29	B3
C012	CIP392657.171	B3	C118	CIP304350.100	LTVR	D107	CIP395109.34	B3
C013	CIP393280.64	B3	C119	CIP304350.118	LTVR	D108	CIP395111.13	B3
C014	CIP391047.34	B3	C120	CIP304350.95	LTVR	D109	CIP395112.19	B3
C015	CIP391058.175	B3	C121	CIP304371.67	LTVR	D110	CIP395112.32	B3
C016	CIP393085.5	B3	C122	CIP304383.41	LTVR	D111	CIP395112.36	B3
C017	CIP398192.213	B3-HT	C123	CIP304383.80	LTVR	D112	CIP395112.6	B3
C018	CIP398098.119	B3-HT	C124	CIP304387.39	LTVR	D113	CIP395123.6	B3
C019	CIP398098.203	B3-HT	C125	CIP304405.47	LTVR	D114	CIP395169.17	B3
C020	CIP398180.253	B3-HT	C127	CIP397077.16	LTVR	D117	CIP395448.1	BW
C021	CIP398180.289	B3-HT	C128	CIP391919.3	BW	D118	CIP396004.225	B3
C024	CIP398193.553	B3-HT	C129	CIP391930.1	BW	D119	CIP396004.263	B3
C025	CIP398203.509	B3-HT	C130	CIP391931.1	BW	D121	CIP396008.104	B3
C026	CIP398208.219	B3-HT	C131	CIP394906.6	BW	D123	CIP396009.258	B3
C027	CIP398208.33	B3-HT	C132	CIP395438.1	BW	D124	CIP396012.266	B3
C028	CIP398208.58	B3-HT	C133	CIP394904.20	BW	D126	CIP396023.109	B3
C029	CIP398208.704	B3-HT	D001	CIP300055.32	LTVR	D127	CIP396026.101	B3
编号 Code	CIP编号 CIP accession	群体 Population	编号 Code	CIP编号 CIP accession	群体 Population	编号 Code	CIP编号 CIP accession	群体 Population
C030	CIP301024.14	B3-LTVR	D002	CIP300063.9	LTVR	D128	CIP396026.103	B3
C031	CIP301029.18	B3-LTVR	D003	CIP300065.4	LTVR	D130	CIP396031.108	B3
C032	CIP301040.63	B3-LTVR	D005	CIP300135.3	LTVR	D131	CIP396031.119	B3
C033	CIP300046.22	LTVR	D007	CIP301023.15	B3-LTVR	D132	CIP396033.102	B3
C034	CIP300048.12	LTVR	D008	CIP301024.95	B3-LTVR	D133	CIP396034.103	B3
C035	CIP300054.29	LTVR	D009	CIP301026.23	B3-LTVR	D134	CIP396034.268	B3
C036	CIP300056.33	LTVR	D011	CIP301041.26	B3-LTVR	D137	CIP396038.101	B3
C037	CIP300063.4	LTVR	D012	CIP301044.36	B3-LTVR	D138	CIP396038.105	B3
C038	CIP300066.11	LTVR	D013	CIP301045.74	B3-LTVR	D139	CIP396038.107	B3
C039	CIP300072.1	LTVR	D014	CIP301055.53	B3-LTVR	D140	CIP396043.226	B3
C040	CIP300093.14	LTVR	D015	CIP301056.54	B3-LTVR	D142	CIP396063.1	B3-LTVR
C041	CIP300099.22	LTVR	D017	CIP303381.106	LTVR	D143	CIP396063.16	B3-LTVR
C042	CIP300101.11	LTVR	D018	CIP303381.30	LTVR	D144	CIP396180.22	B3-LTVR
C043	CIP379706.27	LTVR	D019	CIP304347.6	LTVR	D145	CIP396240.2	B3
C044	CIP385499.11	LTVR	D021	CIP304350.18	LTVR	D147	CIP396241.4	B3
C046	CIP388676.1	LTVR	D022	CIP304350.78	LTVR	D148	CIP396244.12	B3
C047	CIP388972.22	LTVR	D023	CIP304351.109	LTVR	D149	CIP396268.1	B3-LTVR
C048	CIP390478.9	LTVR	D024	CIP304351.31	LTVR	D150	CIP396268.9	B3-LTVR
C049	CIP391207.2	LTVR	D025	CIP304366.46	LTVR	D151	CIP396269.14	B3-LTVR
C050	CIP391382.18	LTVR	D028	CIP304371.58	LTVR	D152	CIP396269.16	B3-LTVR
C051	CIP392781.1	LTVR	D029	CIP304387.17	LTVR	D154	CIP396272.18	B3-LTVR
C052	CIP392797.22	LTVR	D030	CIP304387.92	LTVR	D156	CIP396272.21	B3-LTVR
C053	CIP392822.3	LTVR	D031	CIP304394.56	LTVR	D157	CIP396272.37	B3-LTVR
C054	CIP392973.48	LTVR	D033	CIP304399.5	LTVR	D158	CIP396273.48	B3-LTVR
C056	CIP394034.65	LTVR	D035	CIP304406.31	LTVR	D160	CIP397006.18	LTVR
C057	CIP394034.7	LTVR	D036	CIP374080.5	A	D162	CIP397039.53	LTVR
C058	CIP394579.36	LTVR	D037	CIP377744.1	A	D164	CIP397060.19	LTVR
C059	CIP394600.52	LTVR	D040	CIP381178.14	A	D165	CIP398098.205	B3-HT
C061	CIP394613.139	LTVR	D041	CIP381379.12	A	D166	CIP398098.231	B3-HT
C062	CIP394613.32	LTVR	D042	CIP381381.9	A	D167	CIP398098.570	B3-HT
C063	CIP394614.117	LTVR	D043	CIP381403.16	A	D168	CIP398098.65	B3-HT
C064	CIP394881.8	LTVR	D044	CIP384321.3	A	D169	CIP398180.144	B3-HT
C065	CIP395186.6	LTVR	D047	CIP387164.4	B3	D170	CIP398190.112	B3-HT
C066	CIP395193.6	LTVR	D048	CIP389746.2	B3	D171	CIP398190.200	B3-HT
C067	CIP395195.7	LTVR	D050	CIP391011.17	B3	D172	CIP398190.404	B3-HT
C068	CIP395196.4	LTVR	D051	CIP391046.14	B3	D173	CIP398190.523	B3-HT
C069	CIP395197.5	LTVR	D052	CIP391065.81	B3	D174	CIP398190.530	B3-HT
C070	CIP395432.51	LTVR	D053	CIP391580.30	B3	D175	CIP398190.571	B3-HT
C071	CIP395434.1	LTVR	D054	CIP391585.179	B3	D176	CIP398190.605	B3-HT
C072	CIP395436.8	LTVR	D055	CIP391585.5	B3	D177	CIP398190.615	B3-HT
C076	CIP397012.22	LTVR	D057	CIP392025.7	LTVR	D178	CIP398190.735	B3-HT
C078	CIP397016.7	LTVR	D058	CIP392285.72	BW	D179	CIP398192.41	B3-HT
C079	CIP397029.21	LTVR	D059	CIP392633.64	B3	D180	CIP398192.553	B3-HT
C080	CIP397036.7	LTVR	D060	CIP392634.49	B3	D181	CIP398192.592	B3-HT
C081	CIP397039.51	LTVR	D061	CIP392637.10	B3	D182	CIP398193.158	B3-HT
编号 Code	CIP编号 CIP accession	群体 Population	编号 Code	CIP编号 CIP accession	群体 Population	编号 Code	CIP编号 CIP accession	群体 Population
C082	CIP397044.25	LTVR	D064	CIP392821.1	LTVR	D183	CIP398193.650	B3-HT
C083	CIP397055.2	LTVR	D065	CIP393075.54	B3	D185	CIP398208.29	B3-HT
C084	CIP397065.2	LTVR	D067	CIP393077.54	B3	D186	CIP398208.505	B3-HT
C085	CIP397067.2	LTVR	D068	CIP393079.24	B3	D187	CIP398208.620	B3-HT
C086	CIP397069.5	LTVR	D069	CIP393079.4	B3	D188	CIP398208.670	B3-HT
C087	CIP397073.15	LTVR	D070	CIP393083.2	B3	D189	CIP399001.44	B1
C088	CIP397078.12	LTVR	D071	CIP393084.31	B3	D190	CIP399004.19	B1
C089	CIP397079.26	LTVR	D072	CIP393220.54	B3	D192	CIP399049.14	B1
C090	CIP397079.6	LTVR	D074	CIP393248.55	B3	D193	CIP399049.16	B1
C091	CIP397098.12	LTVR	D075	CIP393280.57	B3	D194	CIP399049.22	B1
C092	CIP397099.6	LTVR	D077	CIP393284.39	B3	D195	CIP399053.11	B1
C093	CIP397100.9	LTVR	D078	CIP393339.242	B3	D196	CIP399053.15	B1
C094	CIP397196.3	LTVR	D079	CIP393349.68	B3	D199	CIP399067.22	B1
C095	CIP397196.8	LTVR	D080	CIP393371.157	B3	D201	CIP399072.28	B1
C096	CIP397197.9	LTVR	D081	CIP393371.159	B3	D203	CIP399075.32	B1
C097	CIP398014.2	LTVR	D082	CIP393371.58	B3	D205	CIP399078.11	B1
C098	CIP388611.22	LTVR	D083	CIP393382.44	B3	D206	CIP399079.22	B1
C099	CIP388615.22	LTVR	D084	CIP393385.39	B3	D207	CIP399083.4	B1
C100	CIP389468.3	LTVR	D085	CIP393385.47	B3	D209	CIP399085.23	B1
C101	CIP390637.1	LTVR	D088	CIP393617.1	LTVR	D210	CIP399085.30	B1
C102	CIP391180.6	LTVR	D089	CIP394223.19	LTVR	D212	CIP694474.33	PREBRED
C103	CIP391533.1	LTVR	D092	CIP394895.7	BW	D214	CIP800048	VARIETY
C104	CIP391724.1	LTVR	D093	CIP394898.13	BW	D216	CIP800923	VARIETY
C105	CIP392032.2	LTVR	D094	CIP394899.5	BW	D217	CIP800959	VARIETY

表1

图1

图2

表2

6个马铃薯晚疫病抗性性状在不同分析模型和显著水平下的显著关联标记"

编号 Number	SNP位点 SNP locus	染色体 Chr.	不同模型、显著水平下与SNP相关联的性状 Traits correlated with SNP under different analytical models and significant level
1	10774833	3	5^d_0.1, 6^d_0.1
2	10774840	3	5^d_0.1
3	10774841	3	5^d_0.1
4	14767856	3	3^{d, e}_0.1, 4^{d, e}_0.1, 6^{d, e}_0.1
5	18132045	3	3^d_0.1, 4^d_0.1
6	32015964	3	3^d_0.1, 4^d_0.1, 6^d_0.1
7	7144093	4	5^d_0.1, 6^d_0.1
8	17868575	4	3^d_0.1, 4^d_0.1
9	31375673	4	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
10	4579130	5	3^{b, c, d}_0.1, 3^{b, c}_0.01, 4^{b, c, d}_0.1, 4^b_0.01
11	2241058	6	1^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.1, 1^{b, c, d}_0.01, 2^{b, c,d}_0.01, 3^d_0.1, 4^d_0.1, 3^d_0.01, 4^d_0.01, 5^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.1, 5^d_0.01, 6^d_0.01
12	2241060	6	1^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.1, 1^{b, c, d}_0.01, 2^{b, c,d}_0.01, 3^d_0.1, 4^d_0.1, 3^d_0.01, 4^d_0.01, 5^{a, b, c, d, e}_0.1, 6^{b, c, d, e}_0.1, 5^d_0.01, 6^d_0.01
13	17610976	7	1^d_0.1, 2^d_0.1, 5^d_0.1, 6^d_0.1, 5^d_0.01
14	37485429	7	1^d_0.1, 2^d_0.1, 5^d_0.1, 6^d_0.1
15	22968094	9	1^d_0.1, 2^d_0.1, 5^d_0.1, 6^d_0.1
16	30491004	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
17	30518418	9	1^{d, e}_0.1, 1^d_0.01, 2^{d, e}_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^{d, e}_0.1, 5^d_0.01, 6^{d, e}_0.1, 6^d_0.01
18	30522602	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^{d, e}_0.1, 5^d_0.01, 6^{d, e}_0.1, 6^d_0.01
19	30542106	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
20	30550212	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
21	30568973	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
22	31001787	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
23	31707832	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
24	32170544	9	1^d_0.1, 2^d_0.1, 3^d_0.1, 4^d_0.1, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
25	32382888	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
26	32442672	9	1^d_0.1
27	32468577	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
28	32627854	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d}_0.01, 3^{b, c, d}_0.1, 3^d_0.01, 4^{b, c, d}_0.1, 4^d_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d}_0.01, 6^{a, b, c, d, e}_0.1, 6^{b, c, d}_0.01
29	32642867	9	1^{a, c, d, e}_0.1, 2^{a, c, d, e}_0.1, 1^d_0.01, 2^d_0.01, 3^d_0.1, 4^d_0.1, 3^d_0.01, 4^d_0.01, 5^{a, d, e}_0.1, 6^{a, d, e}_0.1, 5^{a, d}_0.01, 6^d_0.01
30	32747131	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{b, c, d, e}_0.01, 4^{b, c, d, e}_0.1, 4^{b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{b, c, d, e}_0.01
31	32749768	9	3^d_0.1, 4^d_0.1
32	32749770	9	3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 6^d_0.1
编号 Number	SNP位点 SNP locus	染色体 Chr.	不同模型、显著水平下与SNP相关联的性状 Traits correlated with SNP under different analytical models and significant level
33	32752786	9	1^d_0.1, 2^d_0.1, 3^{b, d}_0.1, 3^d_0.01, 4^{b, d}_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
34	32753035	9	1^d_0.1, 2^d_0.1, 3 ^d_0.1, 3^d_0.01, 4 ^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
35	32973114	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
36	33118463	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
37	33145926	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d}_0.01, 3^{b, c, d}_0.1, 3^{c, d}_0.01, 4^{b, c, d}_0.1, 4^{c, d}_0.01, 5^{a, b, c, d, e}_0.1, 5^{b, c, d}_0.01, 6^{a, b, c, d, e}_0.1, 6^{b, c, d}_0.01
38	33145927	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
39	33148740	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
40	33156262	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
41	33170290	9	1^d_0.1, 2^d_0.1, 5^d_0.1, 6^d_0.1
42	33170293	9	1^d_0.1, 2^d_0.1, 5^d_0.1, 6^d_0.1
43	33211574	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
44	33222811	9	1^{c, d}_0.1, 1^d_0.01, 2^{c, d}_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^{c, d}_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
45	33229209	9	1^d_0.1, 2^d_0.1, 5^d_0.1
46	33230719	9	3^d_0.1, 4^d_0.1, 5^d_0.1, 6^d_0.1
47	33243331	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
48	33244698	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
49	33283731	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
50	33283949	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
51	33288990	9	1^d_0.1, 2^d_0.1, 3^d_0.1, 4^d_0.1, 5^d_0.1, 6^d_0.1
52	33302511	9	1^{b, c, d}_0.1, 1^d_0.01, 2^{b, c, d}_0.1, 2^d_0.01, 3^{b, c, d}_0.1, 3^d_0.01, 4^{b, c, d}_0.1, 4^d_0.01, 5^{b, c, d}_0.1, 5^d_0.01, 6^{b, c, d}_0.1, 6^d_0.01
53	33451096	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
54	33451119	9	1^{b, c, d}_0.1, 1^d_0.01, 2^{b, c, d}_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^{b, c, d}_0.1, 5^d_0.01, 6^{b, c, d}_0.1, 6^d_0.01
55	33480937	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
56	34878453	9	3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 6^d_0.1, 6^d_0.01
57	34898663	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{b, c, d}_0.01, 4^{a, b, c, d, e}_0.1, 4^{b, c, d}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
58	34898695	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{b, c, d, e}_0.1, 3^{b, c, d}_0.01, 4^{b, c, d, e}_0.1, 4^{b, c, d}_0.01, 5^{a, b, c, d, e}_0.1, 5^{b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
59	34908783	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^{a, b, c, d, e}_0.1, 5^d_0.01, 6^{a, b, c, d, e}_0.1, 6^d_0.01
60	34922341	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3 ^d_0.1, 4 ^d_0.1, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
61	34935499	9	1^{a, b, c, d, e}_0.1, 1^{a, c, d}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, c, d}_0.01, 3^{a, d}_0.1, 3^d_0.01, 4^{a, d}_0.1, 4^d_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, c, d}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, d}_0.01
62	34959324	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
编号 Number	SNP位点 SNP locus	染色体 Chr.	不同模型、显著水平下与SNP相关联的性状 Traits correlated with SNP under different analytical models and significant level
63	34964634	9	1^d_0.1, 2^d_0.1
64	34998000	9	1^d_0.1, 2^d_0.1, 5^d_0.1, 6^d_0.1
65	35003777	9	1^d_0.1, 2^d_0.1, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
66	35060347	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
67	35065836	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
68	35097925	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
69	35097926	9	3^d_0.1, 4^d_0.1, 5^d_0.1, 6^d_0.1
70	35114336	9	3^d_0.1, 3^d_0.01, 4^d_0.1, 4^d_0.01, 5^d_0.1, 6^d_0.1
71	35117629	9	1^{a, b, c, d, e}_0.1, 1^{a, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, c, d, e}_0.01, 3^{a, d, e}_0.1, 3^d_0.01, 4^{a,b, d, e}_0.1, 4^d_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, d}_0.01
72	35123523	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
73	35123542	9	1^{a, b, c, d, e}_0.1, 1^{b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{b, c, d}_0.01, 4^{a, b, c, d, e}_0.1, 4^{b, c, d}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
74	35123599	9	3^d_0.1, 5^d_0.1, 6^d_0.1
75	35180852	9	1^d_0.1, 2^d_0.1, 3^d_0.1, 4^d_0.1, 5^d_0.1, 6^d_0.1
76	35212385	9	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3^{b, c, d}_0.1, 3^{b, c, d}_0.01, 4^{b, c, d}_0.1, 4^{b, d}_0.01, 5^{b, c, d}_0.1, 5^d_0.01, 6^{b, c, d}_0.1, 6^d_0.01
77	35212534	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
78	35253233	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, d, e}_0.1, 3^d_0.01, 4^{a, d, e}_0.1, 4^{d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, d, e}_0.01
79	35254558	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d}_0.01, 3^{a,b, c, d, e}_0.1, 3b, c, ^d_0.01, 4^{a, b, c, d, e}_0.1, 4b, c, ^d_0.01, 5^{a, b, c, d, e}_0.1, 5^{b, c, d}_0.01, 6^{a, b, c, d, e}_0.1, 6^{b, c, d}_0.01
80	35256410	9	1^{a, b, c, d, e}_0.1, 1^{a, b, c, d, e}_0.01, 2^{a, b, c, d, e}_0.1, 2^{a, b, c, d, e}_0.01, 3^{a, b, c, d, e}_0.1, 3^{a, b, c, d, e}_0.01, 4^{a, b, c, d, e}_0.1, 4^{a, b, c, d, e}_0.01, 5^{a, b, c, d, e}_0.1, 5^{a, b, c, d, e}_0.01, 6^{a, b, c, d, e}_0.1, 6^{a, b, c, d, e}_0.01
81	8842711	11	1^d_0.1, 2^d_0.1, 3^d_0.1, 3^d_0.01, 4^d_0.1, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01
82	23995788	12	1^d_0.1, 1^d_0.01, 2^d_0.1, 2^d_0.01, 3 ^d_0.1, 4 ^d_0.1, 5^d_0.1, 5^d_0.01, 6^d_0.1, 6^d_0.01

表2

图3

表3

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马铃薯资源晚疫病抗性的全基因组关联分析

Genome-wide association analysis for late blight resistance of potato resources

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