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作物学报 ›› 2022, Vol. 48 ›› Issue (9): 2221-2227.doi: 10.3724/SP.J.1006.2022.11085

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基因聚合选育抗赤霉病小麦新品系百农4299

张一铎1(), 李国强1, 孔忠新1, 王玉泉2, 李小利2, 茹振钢2, 贾海燕1,*(), 马正强1   

  1. 1.南京农业大学作物基因组学与生物信息学中心 / 作物遗传与种质创新国家重点实验室, 江苏南京 210095
    2.河南科技学院小麦中心, 河南新乡 453003
  • 收稿日期:2021-09-29 接受日期:2021-11-29 出版日期:2022-09-12 网络出版日期:2022-12-27
  • 通讯作者: 贾海燕
  • 作者简介:E-mail: 2014201043@njau.edu.cn
  • 基金资助:
    国家重点研发计划项目(2016YFD0101802);国家自然科学基金项目(31930081);国家自然科学基金项目(30430440);现代作物生产(省部共建)协同创建中心

Breeding of FHB-resistant wheat line Bainong 4299 by gene pyramiding

ZHANG Yi-Duo1(), LI Guo-Qiang1, KONG Zhong-Xin1, WANG Yu-Quan2, LI Xiao-Li2, RU Zhen-Gang2, JIA Hai-Yan1,*(), MA Zheng-Qiang1   

  1. 1. Crop Genomics and Bioinformatics Center, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
    2. Center of Wheat Research, Henan Institute of Science and Technology, Xinxiang 450003, Henan, China
  • Received:2021-09-29 Accepted:2021-11-29 Published:2022-09-12 Published online:2022-12-27
  • Contact: JIA Hai-Yan
  • Supported by:
    National Key Research and Development Program of China(2016YFD0101802);National Natural Science Foundation of China(31930081);National Natural Science Foundation of China(30430440);Collaborative Innovation Initiative for Modern Crop Production

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摘要：

小麦赤霉病是一种严重危害小麦生产的真菌性病害, 其抗性由多基因控制, 抗性机制复杂。type I (抗侵入)和type II (抗扩展)是小麦抵御赤霉病侵害的2种最主要抗性类型。在抗赤霉病育种中兼顾2种抗性, 对于保证生产上抗性的稳定和持久有着重要意义。在前期研究中, 作者所在课题组从小麦地方品种望水白中克隆了抗赤霉病扩展的主效QTL Fhb1, 精细定位了Fhb4和Fhb5, 获得了功能性/紧密连锁的分子标记。本研究利用这些标记, 以小麦品系NMAS022作为供体亲本, 现代小麦品种百农4199作为受体亲本, 通过分子标记辅助回交育种方法选育成了聚合望水白Fhb1、Fhb4、Fhb5的小麦新品系百农4299。与百农4199相比, 百农4299在2年的田间试验中type I抗性至少增加了73%~74%, type II抗性至少增加了83%~88% (以病小穗数计), 并且产量潜力也得到了提高。上述结果证明了通过分子标记辅助选择聚合不同类型抗赤霉病QTL以提高小麦赤霉病抗性的可行性。抗赤霉病小麦品系百农4299有望成为一个新的抗赤霉病小麦品种。

关键词: 小麦, 赤霉病, 抗病基因聚合, MAS, 百农4299

Abstract:

Fusarium head blight (FHB) is a devastating fungal disease in wheat production. Wheat FHB resistance is controlled by multiple genes and has complicated resistance mechanisms. Type I (resistance to invasion) and type II (resistance to expansion) are two main resistance types of wheat against FHB. Combining both types of FHB resistance in breeding is vitally important for the resistance durability and stability of cultivars. In fine mapping and cloning of type I resistance QTL Fhb4 and Fhb5 and type II resistance QTL Fhb1 in wheat landrace Wangshuibai, functional/tightly-linked molecular markers for them had been obtained. In this study, a new wheat line named Bainong 4299 was bred after introduction of these QTL from NMAS022 with the help of these markers and using modern wheat variety Bainong 4199 as the recipient parent. Compared with Bainong 4199, Bainong 4299 increased type I resistance by at least 73% to 74% and type II resistance by 83% to 88% increase (in terms of the number of diseased spikelets per spike) in two field trials. Moreover, its yield potential had moderate elevation. In conclusion, this study provided another successful illustration of marker-assisted selection and pyramiding of FHB QTL in improving wheat FHB resistance. Bainong 4299 had the potential to become a new FHB resistance cultivar.

Key words: wheat, Fusarium head blight, pyramiding of resistance genes, MAS, Bainong 4299

图1

百农4199、NMAS022、百农4299中Fhb1、Fhb4、Fhb5标记检测结果 M: DNA marker, 图片左侧的数字为条带分子量大小(单位: bp); 1: 百农4199; 2: NMAS022; 3: 百农4299; 望水白类型的目标条带分别在图中用箭头标出, 图片下方分别为引物名称。"

图2

百农4299的选育过程(a)及其供体亲本NMAS022 (b) n: 群体大小; 图片(b)拍摄于2020年5月4日, 江苏淮安。"

表1

百农4299及其轮回亲本的赤霉病抗性鉴定结果"

环境
Environment		 材料
Material		 赤霉病严重度
FHB severity		 病穗率
Percentage of infected spikes (%)		 病小穗数
Number of diseased spikelets		 病轴长
Length of diseased rachides (cm)
2019-2020淮安
2019-2020 Huai’an		 百农4199 Bainong 4199 2.23±0.11 38.5±1.8 5.8±0.6 4.1±0.5
百农4299 Bainong 4299 0.10±0.02** 10.2±2.2** 1.0±0.0** 0.8±0.1**
2020-2021南京
2020-2021 Nanjing		 百农4199 Bainong 4199 3.44±0.14 42.3±2.1 8.2±0.1 4.1±0.1
百农4299 Bainong 4299 0.11±0.01** 11.5±1.3** 1.0±0.0** 0.6±0.0**

图3

百农4299 (左)及其轮回亲本百农4199 (右)在田间自然发病状态下的赤霉病抗性表现 图片拍摄于开花后21 d, 2021年江苏南京。"

表2

百农4299及其轮回亲本的农艺性状和产量鉴定结果"

环境
Environment		 材料
Material		 株高
Plant height (cm)		 穗粒数
No. of kernels per spike		 单株穗数
No. of spikes per plant		 千粒重
1000-kernel weight (g)		 1 m2产量
Yield per square meter (g)
2019-2020淮安
2019-2020 Huai’an		 百农4199 Bainong 4199 80.2±1.2 54.4±1.8 12.1±0.6 44.2±0.4 984.5±3.5
百农4299 Bainong 4299 84.2±0.9** 55.0±1.9 12.0±0.9 48.4±0.5** 1002.0±4.2*
2020-2021淮安
2020-2021 Huai’an		 百农4199 Bainong 4199 82.0±1.2 42.3±1.6 4.4±0.8 46.3±0.4 845.9±5.8
百农4299 Bainong 4299 86.9±1.7** 39.0±1.6** 5.3±1.2 44.7±0.2** 874.8±3.4*
2020-2021南京
2020-2021 Nanjing		 百农4199 Bainong 4199 72.6±1.8 42.5±0.8 3.8±0.1 41.5±0.2 490±5.2
百农4299 Bainong 4299 72.0±1.2 37.1±1.1** 4.6±0.3 42.8±0.2** 520±4.7*
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