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作物学报

作物学报 ›› 2022, Vol. 48 ›› Issue (8): 1905-1913.doi: 10.3724/SP.J.1006.2022.11069

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦顶端小穗退化突变体asd1基因定位

杜启迪1,2(), 郭会君2, 熊宏春2, 谢永盾2, 赵林姝2, 古佳玉2, 赵世荣2, 丁玉萍2, 宋希云1, 刘录祥2,*()   

  1. 1青岛农业大学生命科学学院, 山东青岛 266109
    2中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学 工程 / 国家农作物航天诱变技术改良中心, 北京 100081
  • 收稿日期:2021-08-06 接受日期:2021-11-29 出版日期:2022-08-12 网络出版日期:2021-12-13
  • 通讯作者: 刘录祥
  • 作者简介:E-mail: duqididd@163.com
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-03);中国农业科学院科技创新工程重大科研任务(CAAS-ZDRW202002)

Gene mapping of apical spikelet degeneration mutant asd1 in wheat

DU Qi-Di1,2(), GUO Hui-Jun2, XIONG Hong-Chun2, XIE Yong-Dun2, ZHAO Lin-Shu2, GU Jia-Yu2, ZHAO Shi-Rong2, DING Yu-Ping2, SONG Xi-Yun1, LIU Lu-Xiang2,*()   

  1. 1College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, Shandong, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
  • Received:2021-08-06 Accepted:2021-11-29 Published:2022-08-12 Published online:2021-12-13
  • Contact: LIU Lu-Xiang
  • Supported by:
    China Agriculture Research System(CARS-03);Agricultural Science and Technology Innovation Program(CAAS-ZDRW202002)

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摘要：

穗粒数是小麦产量三要素建成的关键因子, 深入挖掘穗部发育调控基因有助于培育高产小麦品种。以小麦品种京411为野生型, 经EMS诱变获得了表型稳定的小穗退化突变体asd1 (apical spikelet degeneration 1)。该突变体表现顶端小穗明显退化, 穗长缩短了约40%, 结实小穗数减少了约35%, 穗粒数显著减少了54%, 同时株高也明显降低。利用京411×asd1遗传群体的F2和F3代表型数据分析表明, 顶端小穗退化性状受1对主效隐性基因控制。采用混合群体分离分析法(BSA), 结合测序所得SNP位点, 在7A染色体上开发了7个KASP标记, 将目标突变基因定位在7A染色体短臂9.91 Mb物理区间内, 遗传距离为17.62 cM, 推断该区段存在一个新的控制小麦花器官发育及穗部形态发育的重要基因。本研究所鉴定的小麦穗发育控制区段有助于深入解析小麦小穗形成的遗传基础, 为进一步揭示小麦产量形成的分子机理提供突变基因。

关键词: 小麦, 顶端小穗退化, 遗传分析, 基因定位

Abstract:

The number of grains per spike is one of the three key factors for yield and production in wheat. It is helpful to cultivate high-yield wheat varieties to elaborate the regulation pathway of spike development. A stable mutant of apical spikelet degeneration (asd1) was derived by EMS treatment, the wheat variety Jing 411 was used as wild type. The mutant asd1 showed that the apical spikelets were significantly degenerated, the spike length was shortened by 40%, the number of spikelets per spike was reduced by 35%, the number of grains per spike was significantly reduced by 54%, and the plant height was also significantly reduced. A genetic segregation population, Jing 411 × asd1, was developed, and phenotypic performances of both F2 and F3 were investigated in field. It showed that the degeneration of apical spikelet was controlled by a recessive gene. Using Bulked Segregant Analysis (BSA) and exon capture, SNPs were obtained and 7 KASP markers were further developed. The target mutation gene was mapped on chromosome 7A and narrowed down to a 9.91 Mb region on 7AS, which represented a genetic distance of 17.62 cM. The region had not yet been reported in regulating spikelet development and the target mutant gene might be a novel gene that controls the development of spike morphology in wheat, which will bring new insights for further understanding of the genetic basis of spikelet development in wheat.

Key words: wheat, apical spikelet degeneration, genetic analysis, gene mapping

图1

突变体asd1的小穗退化表型与穗长和株高 (A)~(D)小花原基分化期至四分体时期的麦穗(标尺为1 mm); (E) 孕穗期麦穗(标尺为1 cm); (F) 灌浆期麦穗(标尺为2 cm); (G) 灌浆期单株(标尺为15 cm); (H) 穗长; (I) 株高。图中左侧表示野生型, 右侧表示突变体。"

图2

突变体asd1顶端小穗中花器官退化 (A) 突变体和野生型顶端小穗至第8小穗的解剖图; (B) 碘-碘化钾染色测定花粉活力; (C) 结实小穗数; (D) 每穗粒数。"

附表1

J411×asd1的F2群体和F3家系中顶端小穗退化单株表型"

单株
Plant		 F2表型
Phenotype of F2		 F2小穗数
Number of spikelet of F2		 F3小穗数
Number of spikelet of F3		 F3小穗数平均值±标准差
Average ± Standard Deviation		 F3表型
Phenotype of F3
重复一
Repeat one		 重复二
Repeat two		 重复一
Repeat one		 重复二
Repeat two		 重复三
Repeat three		 重复四
Repeat four
京411 W 20 22 19 17 19 18 18.3±0.96 W
京411 W 18 19 17 17 17 18 17.3±0.50 W
asd1 M 9 12 13 10 11 9 10.8±1.71 M
asd1 M 11 11 12 10 8 9 9.8±1.71 M
1 M 12 13 11 13 10 11 11.3±1.26 M
2 M 13 12 10 12 10 9 10.3±1.26 M
3 M 12 13 9 11 13 10 10.8±1.71 M
4 M 13 11 12 13 11 11 11.8±0.96 M
5 M 11 12 10 11 9 9 9.8±0.96 M
6 M 13 12 9 10 10 11 10.0±0.82 M
7 M 13 10 8 10 12 12 10.5±1.91 M
8 M 13 12 12 10 12 12 11.5±1.00 M
9 M 13 11 11 13 10 11 11.3±1.26 M
10 M 14 9 8 8 10 10 9.0±1.15 M
11 M 14 9 10 10 8 9 9.3±0.96 M
12 M 12 12 10 10 12 11 10.8±0.96 M
13 M 14 14 10 11 11 12 11.0±0.82 M
14 M 14 13 10 10 11 12 10.8±0.96 M
15 M 11 13 10 11 11 13 11.3±1.26 M
16 M 12 10 10 11 10 11 10.5±0.58 M
17 M 14 14 9 10 11 10 10.0±0.82 M
18 M 12 10 9 11 8 9 9.3±1.26 M
19 M 13 13 12 11 8 10 10.3±1.71 M
20 M 11 11 8 10 10 10 9.5±1.00 M
21 M 13 14 10 12 12 12 11.5±1.00 M
22 M 10 12 10 10 10 11 10.3±0.50 M
23 M 12 10 8 9 8 8 8.3±0.50 M
24 M 13 12 9 10 11 12 10.5±1.29 M
25 M 13 13 9 10 12 12 10.8±1.50 M
26 M 11 11 10 10 10 11 10.3±0.50 M
27 M 12 12 8 10 8 8 8.5±1.00 M
28 M 11 11 7 9 12 10 9.5±2.08 M
29 M 11 8 9 10 9 9 9.3±0.50 M
30 M 10 11 11 12 10 10 10.8±0.96 M
31 M 14 14 10 11 11 12 11.0±0.82 M
32 M 13 12 9 9 10 11 9.8±0.96 M
33 M 14 14 9 9 11 12 10.3±1.50 M
34 M 14 12 12 13 9 10 11.0±1.83 M
35 M 11 14 9 9 11 12 10.3±1.50 M
36 M 12 14 10 11 11 12 11.0±0.82 M
37 M 13 13 9 10 13 12 11.0±1.83 M
38 M 13 14 9 9 10 12 10.0±1.41 M
39 M 14 12 11 11 11 12 11.3±0.50 M
40 M 14 12 13 12 10 12 11.8±1.26 M
41 M 13 10 8 10 8 10 9.0±1.15 M
42 M 14 13 11 11 11 12 11.3±0.50 M
43 M 14 10 10 11 9 10 10.0±0.82 M
44 M 13 13 9 9 10 11 9.8±0.96 M
45 M 13 12 8 9 8 8 8.3±0.50 M
46 M 13 13 8 8 10 12 9.5±1.91 M
47 M 14 14 9 12 9 11 10.3±1.50 M
48 M 9 11 10 11 11 13 11.3±1.26 M
49 M 12 12 8 10 10 12 10.0±1.63 M
50 M 14 11 9 11 11 11 10.5±1.00 M
51 M 14 12 10 11 9 10 10.0±0.82 M
52 M 12 10 10 11 11 11 10.8±0.50 M
53 M 13 14 8 8 10 12 9.5±1.91 M
54 M 13 12 7 9 9 9 8.5±1.00 M
55 M 13 9 12 11 8 8 9.8±2.06 M
56 M 13 12 10 11 8 10 9.8±1.26 M
57 M 10 11 11 11 10 10 10.5±0.58 M
58 M 11 11 9 9 9 12 9.8±1.50 M
59 M 13 11 9 10 9 11 9.8±0.96 M
60 M 12 12 7 8 9 11 8.8±1.71 M
61 M 14 14 9 10 12 12 10.8±1.50 M
62 M 13 13 10 10 10 11 10.3±0.50 M
63 M 14 12 11 12 13 11 11.8±0.96 M
64 M 12 11 9 10 8 9 9.0±0.82 M
65 M 8 11 8 10 9 9 9.0±0.82 M
66 M 13 13 9 10 9 10 9.5±0.58 M
67 M 13 14 10 13 10 10 10.8±1.50 M
68 M 13 13 10 11 10 12 10.8±0.96 M
69 M 11 12 11 13 11 12 11.8±0.96 M
70 M 14 13 10 10 8 9 9.3±0.96 M
71 M 12 12 11 11 8 10 10.0±1.41 M
72 M 12 12 11 12 9 10 10.5±1.29 M
73 M 13 13 10 12 8 9 9.8±1.71 M
74 M 10 13 10 12 10 11 10.8±0.96 M
75 M 11 10 7 10 9 9 8.8±1.26 M
76 M 12 10 9 9 10 11 9.8±0.96 M
77 M 10 12 7 9 10 10 9.0±1.41 M
78 M 12 10 10 12 9 11 10.5±1.29 M
79 M 11 10 10 11 11 12 11.0±0.82 M
80 M 10 12 10 11 10 11 10.5±0.58 M
81 M 11 12 10 10 11 12 10.8±0.96 M
82 M 13 11 11 11 12 12 11.5±0.58 M
83 M 12 12 8 10 9 12 9.8±1.71 M
84 M 11 13 8 11 9 9 9.3±1.26 M
85 M 13 13 11 12 10 11 11.0±0.82 M
86 M 11 10 8 9 8 8 8.3±0.50 M
87 M 12 12 10 10 11 12 10.8±0.96 M
88 M 14 12 8 8 11 10 9.3±1.50 M
89 M 12 14 12 13 10 12 11.8±1.26 M
90 M 12 11 9 10 8 9 9.0±0.82 M
91 M 14 13 10 11 10 11 10.5±0.58 M
92 M 14 13 14 18 16 16 16.0±1.63 H
93 M 13 11 9 10 11 12 10.5±1.29 M
94 M 11 13 8 8 10 11 9.3±1.50 M
95 M 11 12 10 11 8 10 9.8±1.26 M
96 M 13 11 7 7 8 11 8.3±1.89 M
97 M 12 11 9 10 11 12 10.5±1.29 M
98 M 12 11 10 10 9 9 9.5±0.58 M
99 M 13 13 11 11 8 10 10.0±1.41 M
100 M 12 13 11 11 10 10 10.5±0.58 M
101 M 12 12 10 11 8 10 9.8±1.26 M
102 M 14 10 9 9 9 11 9.5±1.00 M
103 M 10 10 10 11 11 13 11.3±1.26 M
104 M 13 10 8 10 8 9 8.8±0.96 M
105 M 13 10 7 8 11 11 9.3±2.06 M
106 M 11 9 10 11 7 8 9.0±1.83 M
107 M 11 13 10 11 9 10 10.0±0.82 M
108 M 10 10 10 10 8 8 9.0±1.15 M
109 M 12 14 6 8 11 11 9.0±2.45 M
110 M 12 12 10 11 10 10 10.3±0.50 M
111 M 12 12 9 10 8 9 9.0±0.82 M
112 M 10 10 8 9 9 10 9.0±0.82 M
113 M 12 11 11 12 10 11 11.0±0.82 M
114 M 11 11 11 12 11 11 11.3±0.50 M
115 M 11 12 11 12 8 8 9.8±2.06 M
116 M 13 12 10 11 9 10 10.0±0.82 M
117 M 10 11 10 11 10 11 10.5±0.58 M
118 M 11 11 10 11 6 9 9.0±2.16 M
119 M 13 14 8 9 8 9 8.5±0.58 M
120 M 10 12 10 10 10 12 10.5±1.00 M
121 M 14 14 8 9 8 9 8.5±0.58 M
122 M 11 9 11 12 10 11 11.0±0.82 M
123 M 10 8 8 8 8 9 8.3±0.50 M
124 M 12 11 10 10 8 9 9.3±0.96 M
125 M 13 13 10 11 9 9 9.8±0.96 M
126 M 10 12 10 11 10 10 10.3±0.50 M
127 M 13 12 10 12 10 11 10.8±0.96 M
128 M 11 10 10 12 10 10 10.5±1.00 M
129 M 13 12 10 11 9 11 10.3±0.96 M
130 M 12 12 9 11 11 12 10.8±1.26 M
131 M 14 14 8 10 9 11 9.5±1.29 M
132 M 14 11 10 10 9 10 9.8±0.50 M
133 M 11 12 11 12 9 9 10.3±1.50 M
134 M 12 11 9 10 8 8 8.8±0.96 M
135 M 10 11 8 10 8 8 8.5±1.00 M
136 M 13 13 10 10 8 10 9.5±1.00 M
137 M 12 14 11 12 12 12 11.8±0.50 M
138 M 9 12 10 11 11 11 10.8±0.50 M
139 M 12 11 8 11 9 12 10.0±1.83 M
140 M 10 11 10 10 8 9 9.3±0.96 M
141 M 13 12 10 11 9 10 10.0±0.82 M
142 M 14 11 10 11 9 10 10.0±0.82 M
143 M 12 13 10 11 9 10 10.0±0.82 M
144 M 10 11 6 6 11 8 7.8±2.36 M
145 M 14 13 10 11 6 9 9.0±2.16 M
146 M 11 13 11 12 9 10 10.5±1.29 M
147 M 12 12 13 12 10 12 11.8±1.26 M
148 M 14 13 11 9 9 11 10.0±1.15 M
149 M 13 12 9 11 10 10 10.0±0.82 M
150 M 12 14 9 8 8 8 8.3±0.50 M
151 M 12 12 9 10 10 11 10.0±0.82 M
152 M 14 11 6 11 8 9 8.5±2.08 M
153 M 14 11 9 11 8 9 9.3±1.26 M
154 M 12 9 5 10 8 8 7.8±2.06 M
155 M 12 13 10 11 8 9 9.5±1.29 M
156 M 13 13 8 10 9 8 8.8±0.96 M
157 M 11 10 10 10 7 10 9.3±1.50 M
158 M 11 12 8 8 8 8 8.0±0.00 M
159 M 12 12 10 10 9 10 9.8±0.50 M
160 M 11 12 11 10 9 9 9.8±0.96 M
161 M 10 11 10 11 8 9 9.5±1.29 M
162 M 14 13 8 11 7 10 9.0±1.83 M
163 M 12 12 9 10 10 11 10.0±0.82 M
164 M 14 11 7 9 7 9 8.0±1.15 M
165 M 13 15 17 15 14 15 15.3±1.26 H
166 M 10 10 10 12 10 10 10.5±1.00 M
167 M 11 10 10 11 6 8 8.8±2.22 M
168 M 11 13 9 10 10 11 10.0±0.82 M
169 M 10 8 7 9 10 12 9.5±2.08 M
170 M 11 13 11 12 9 9 10.3±1.50 M
171 M 14 12 8 10 9 10 9.3±0.96 M
172 M 11 13 10 10 9 9 9.5±0.58 M
173 M 14 11 12 11 12 12 11.8±0.50 M
174 M 12 14 12 12 10 11 11.3±0.96 M
175 M 14 14 9 9 11 12 10.3±1.50 M
176 M 13 14 10 10 10 10 10.0±0.00 M
177 M 10 12 8 10 8 9 8.8±0.96 M
178 M 8 11 8 9 8 9 8.5±0.58 M
179 M 10 9 8 9 8 9 8.5±0.58 M
180 M 11 10 7 8 8 10 8.3±1.26 M
181 M 13 12 9 10 9 9 9.3±0.50 M
182 M 11 13 8 9 11 11 9.8±1.50 M
183 M 12 13 11 12 12 13 12.0±0.82 M
184 M 14 13 8 9 10 11 9.5±1.29 M
185 M 11 12 10 11 12 12 11.3±0.96 M
186 M 10 11 12 13 12 13 12.5±0.58 M
187 M 11 12 9 10 8 8 8.8±0.96 M
188 M 11 11 10 11 9 10 10.0±0.82 M
189 M 13 14 6 8 9 8 7.8±1.26 M
190 M 14 14 9 10 6 8 8.3±1.71 M
191 M 12 10 8 8 8 8 8.0±0.00 M
192 M 13 11 7 9 8 9 8.3±0.96 M
193 M 12 11 9 9 9 10 9.3±0.50 M
194 M 12 10 8 9 8 9 8.5±0.58 M
195 M 8 11 6 7 6 7 6.5±0.58 M
196 M 13 12 8 9 8 9 8.5±0.58 M
197 M 12 12 9 9 10 11 9.8±0.96 M
198 M 14 12 9 10 10 11 10.0±0.82 M
199 M 11 13 11 11 8 9 9.8±1.50 M
200 M 12 12 9 9 6 8 8.0±1.41 M
201 M 9 10 11 11 10 11 10.8±0.50 M
202 M 6 8 9 10 8 9 9.0±0.82 M
203 M 12 10 10 11 10 11 10.5±0.58 M
204 M 13 13 13 13 13 13 13.0±0.00 M
205 M 14 12 9 10 11 12 10.5±1.29 M
206 M 12 12 12 12 12 12 12.0±0.00 M
207 M 14 11 11 12 10 11 11.0±0.82 M
208 M 13 10 11 10 10 11 10.5±0.58 M
209 M 13 11 8 10 9 10 9.3±0.96 M
210 M 10 12 10 10 11 11 10.5±0.58 M
211 M 14 14 12 12 10 11 11.3±0.96 M
212 M 13 14 9 11 9 11 10.0±1.15 M
213 M 12 13 10 11 9 10 10.0±0.82 M
214 M 10 11 9 9 8 9 8.8±0.50 M
215 M 14 11 11 11 10 10 10.5±0.58 M
216 M 14 11 8 8 7 8 7.8±0.50 M
217 M 13 11 11 12 10 11 11.0±0.82 M
218 M 12 14 9 10 10 10 9.8±0.50 M
219 M 11 13 8 9 8 8 8.3±0.50 M
220 M 12 11 11 12 8 9 10.0±1.83 M
221 M 13 14 10 11 9 10 10.0±0.82 M
222 M 13 14 9 9 9 10 9.3±0.50 M
223 M 13 10 8 9 9 10 9.0±0.82 M
224 M 14 12 9 10 9 10 9.5±0.58 M
225 M 14 12 7 8 8 8 7.8±0.50 M
226 M 12 13 11 7 10 10 9.5±1.73 M
227 M 15 15 15 14 17 15 15.3±1.26 H
228 M 14 14 9 9 8 9 8.8±0.50 M
229 M 11 14 9 10 9 10 9.5±0.58 M
230 M 8 11 8 8 10 12 9.5±1.91 M
231 M 14 12 7 8 8 9 8.0±0.82 M
232 M 9 10 9 11 9 10 9.8±0.96 M
233 M 10 12 8 10 10 11 9.8±1.26 M
234 M 14 11 8 9 10 10 9.3±0.96 M
235 M 14 12 8 9 8 9 8.5±0.58 M
236 M 14 13 8 9 8 9 8.5±0.58 M
237 M 11 9 9 9 7 9 8.5±1.00 M
238 M 11 12 11 11 8 9 9.8±1.50 M
239 M 11 14 9 10 9 11 9.8±0.96 M
240 M 14 14 15 15 18 14 15.5±1.73 H
241 M 11 11 8 10 7 8 8.3±1.26 M
242 M 14 12 10 11 9 11 10.3±0.96 M
243 M 13 14 9 10 11 11 10.3±0.96 M
244 M 10 10 8 10 10 10 9.5±1.00 M
245 M 11 10 9 10 10 10 9.8±0.50 M
246 M 10 10 8 9 10 11 9.5±1.29 M
247 M 11 10 6 10 10 10 9.0±2.00 M
248 M 9 9 9 9 8 9 8.8±0.50 M
249 M 14 13 9 11 10 10 10.0±0.82 M
250 M 14 11 8 9 9 10 9.0±0.82 M
251 M 13 11 11 11 9 9 10.0±1.15 M
252 M 12 12 6 7 7 8 7.0±0.82 M
253 M 9 12 8 9 11 12 10.0±1.83 M
254 M 12 12 8 10 8 9 8.8±0.96 M
255 M 13 11 10 10 9 10 9.8±0.50 M
256 M 13 11 9 10 9 10 9.5±0.58 M
257 M 12 12 10 10 9 10 9.8±0.50 M
258 M 10 10 7 8 7 8 7.5±0.58 M
259 M 14 12 8 10 9 10 9.3±0.96 M
260 M 11 12 8 8 8 9 8.3±0.50 M
261 M 12 10 8 9 10 11 9.5±1.29 M
262 M 10 11 9 9 8 8 8.5±0.58 M
263 M 10 11 7 8 10 11 9.0±1.83 M
264 M 12 10 8 8 9 10 8.8±0.96 M
265 M 13 11 8 10 8 8 8.5±1.00 M
266 M 11 11 9 9 9 9 9.0±0.00 M
267 M 11 14 9 10 10 10 9.8±0.50 M
268 M 13 10 7 8 11 11 9.3±2.06 M
269 M 12 11 9 10 9 9 9.3±0.50 M
270 M 11 13 9 9 9 9 9.0±0.00 M
271 M 8 11 7 8 6 9 7.5±1.29 M
272 M 12 12 8 10 10 11 9.8±1.26 M
273 M 11 12 7 9 9 11 9.0±1.63 M
274 M 12 13 10 10 8 8 9.0±1.15 M
275 M 12 13 8 8 8 9 8.3±0.50 M
276 M 13 13 15 13 18 15 15.3±2.06 H
277 M 13 13 14 14 18 16 15.5±1.91 H
278 M 12 10 9 9 9 10 9.3±0.50 M
279 M 12 13 9 10 7 9 8.8±1.26 M
280 M 14 12 9 10 10 10 9.8±0.50 M
281 M 12 11 9 10 8 8 8.8±0.96 M
282 M 13 12 9 9 9 10 9.3±0.50 M

表1

J411×asd1 F2和F3群体中小穗退化表型单株与非退化表型单株卡方分析"

世代
Generation		 表型
Phenotype		 观察值
Observed count
(O)		 期望值
Expected count
(E)		 (O-E)2/E χ2 P (df=1)
F2 退化表型 Degenerative phenotype 281 296 0.76 1.01 0.315
非退化表型Nondegenerative phenotype 902 887 0.25
合计 Total 1183 1183 1.01
F3 退化表型 Degenerative phenotype 276 296 1.35 3.15 0.076
非退化表型Nondegenerative phenotype 927 887 1.80
合计 Total 1183 1183 3.15

附表2

J411×asd1分离群体在子代与亲本间差异SNP"

染色体
Chr.		 SNP统计
Number of SNPs		 双亲间SNP
SNPs between parents		 F2子代SNP
SNPs between F2 bulks		 双亲与子代间综合SNP
SNPs between parents and bulks		 SNP分布
Distribution of SNPs
1A 713 445 184 34 均匀分布 Uniform distribution
1B 954 581 283 43 均匀分布 Uniform distribution
1D 478 368 111 29 均匀分布 Uniform distribution
2A 847 570 235 60 均匀分布 Uniform distribution
2B 1035 633 325 54 均匀分布 Uniform distribution
2D 768 542 232 39 均匀分布 Uniform distribution
3A 769 517 194 39 均匀分布 Uniform distribution
3B 1060 648 343 85 均匀分布 Uniform distribution
3D 567 389 191 39 均匀分布 Uniform distribution
4A 776 516 215 43 均匀分布 Uniform distribution
4B 600 395 171 36 均匀分布 Uniform distribution
4D 449 332 96 20 均匀分布 Uniform distribution
5A 764 499 195 34 均匀分布 Uniform distribution
5B 974 622 323 57 均匀分布 Uniform distribution
5D 557 392 135 37 均匀分布 Uniform distribution
6A 654 426 200 23 均匀分布 Uniform distribution
6B 1023 604 293 53 均匀分布 Uniform distribution
6D 429 314 104 39 均匀分布 Uniform distribution
7A 1089 681 370 87 富集于染色体短臂末端Enriched at the end of 7AS
7B 909 535 283 39 均匀分布 Uniform distribution
7D 762 581 151 42 均匀分布 Uniform distribution

图3

asd1的主效突变基因位于7AS染色体上 (A) ED关联分析图。(B) 7AS染色体上0~60 Mb区间内SNP统计。"

附表3

KASP标记序列信息"

标记
Marker		 正向引物
Forward primer (5′-3′)		 反向引物
Reverse primer (5′-3′)
SP1 TCCAGCAAGTTGTAATCTGCATG CTGACCGTCCGTCCCCTA
TCCAGCAAGTTGTAATCTGCATA
SP2 CAGTGTCCTCGAGCTGCG ATGGCAAAAACGTAACGACC
CAGTGTCCTCGAGCTGCA
SP3 CCGTGTGCATCGACAAGCTG TGGGACGTGAAAGTGGTCGT
CCGTGTGCATCGACAAGCTA
SP4 CCTGAGCAGTATCTTTCCATCTTT TCTGCCAAGACAAGAATGCA
CCTGAGCAGTATCTTTCCATCTTC
SPT82 GGTCTCGGACATGAGCTTCTCG CCGACGAGTTCATCTCCTCCT
GGTCTCGGACATGAGCTTCTCA
SP6 GTGAGGTCGCTGAACTTGC CCGGTACCTCATCGAGTACAG
GTGAGGTCGCTGAACTTGT
SP7 GCCCTAACCTCCCCTGGC CTTTGTGCGTGGCTGATG
GCCCTAACCTCCCCTGGT

图4

利用KASP标记在7A染色体上构建遗传连锁图谱 (A) asd1突变基因遗传图谱, 橙色横线代表预测目标基因所在位置。(B) 基于J411×asd1 F2群体小穗顶端退化为表型检测到的LOD曲线。"

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