欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2023, Vol. 49 ›› Issue (9): 2433-2445.doi: 10.3724/SP.J.1006.2023.23071

所属专题： 玉米：遗传育种·种质资源·分子遗传学

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米转录因子ZmEREB211对非生物逆境胁迫的应答

艾蓉1,2(), 张春2(), 悦曼芳2, 邹华文1,*(), 吴忠义2,*()   

  1. 1长江大学农学院, 湖北荆州434025
    2北京市农林科学院 / 生物技术研究所 / 农业基因资源与生物技术北京市重点实验室, 北京100097
  • 收稿日期:2022-10-21 接受日期:2023-02-21 出版日期:2023-09-12 网络出版日期:2023-03-01
  • 通讯作者: *邹华文, E-mail: zouhuawen@yangtzeu.edu.cn; 吴忠义, E-mail: zwu22@126.com
  • 作者简介:艾蓉, E-mail: airong202203@163.com
    张春, E-mail: spring2007318@163.com第一联系人：**同等贡献
  • 基金资助:
    国家自然科学基金项目(32001430);国家自然科学基金项目(32171952);国家自然科学基金项目(31971839);北京市农林科学院科技创新能力建设专项(KJCX20220402)

Response of maize transcriptional factor ZmEREB211 to abiotic stress

AI Rong1,2(), ZHANG Chun2(), YUE Man-Fang2, ZOU Hua-Wen1,*(), WU Zhong-Yi2,*()   

  1. 1College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
    2Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, China
  • Received:2022-10-21 Accepted:2023-02-21 Published:2023-09-12 Published online:2023-03-01
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(32001430);National Natural Science Foundation of China(32171952);National Natural Science Foundation of China(31971839);Beijing Academy of Agricultural and Forestry Sciences(KJCX20220402)

RichHTML

31

PDF

458

摘要：

AP2/ERF (APETALA2/ethylene-responsive factor)转录因子是植物中最大的转录因子家族之一, 在调控植物生长发育和响应逆境胁迫等方面起重要作用。探究玉米(Zea mays L.) AP2/ERF家族基因功能将为玉米新种质创制提供重要的基因资源。本研究克隆获得了ZmEREB211 (Gene ID: 103647485)基因, 利用生物信息学分析、实时荧光定量PCR等技术对该基因的基本特性、组织表达特性及响应逆境胁迫表达模式等进行了分析; 对转基因拟南芥株系进行了相应逆境胁迫处理和表型鉴定。结果显示: 该基因只包含1个外显子, cDNA全长为792 bp, 编码263个氨基酸; ZmEREB211蛋白分子量为27.9 kD, 理论等电点为6.01, 具有AP2家族所特有的保守结构域; ZmEREB211基因在玉米根系中的表达量最高, 且在幼根中的表达量高于成熟根中的表达量; 同时该基因在脱水、高盐、干旱和低温等处理条件下均有不同程度的诱导表达。在分别含有不同浓度梯度的NaCl、甘露醇(mannitol)和茉莉酸(jasmonic acid, JA)的1/2 MS培养基上, 转ZmEREB211基因拟南芥株系的根长显著长于野生型。在干旱和高盐处理下, 盆栽转基因拟南芥株系较野生型株系表现出更强的耐受性, 且苗期的绿叶数显著多于野生型, 过氧化物酶(POD)活性和叶绿素含量均显著高于野生型。研究表明ZmEREB211可能参与调控玉米根系生长发育, 对高盐、干旱、渗透等逆境胁迫及JA激素处理均能起到正向的调控作用。本研究为进一步解析ZmEREB211在玉米中的生物学功能提供了重要的参考依据。

关键词: 玉米, ZmEREB211, 转录因子, 异源表达, 根系, 逆境胁迫

Abstract:

AP2/ERF (APETALA2/ethylene-responsive factor) transcription factor is one of the largest transcription factor families in plants, which plays an important role in regulating plant growth and development and responding to various stresses. Exploring the function of AP2/ERF family genes in maize (Zea mays L.) will provide the important genetic resources for the creation of new maize germplasm. In this study, ZmEREB211 (Gene ID: 103647485) gene was cloned and its basic characteristics, tissue expression characteristics, and the relative expression patterns in response to stress were analyzed by bioinformatics and qRT-PCR. The transgenic Arabidopsis lines were subjected to corresponding stress treatment and phenotypic identification. The results showed that the gene contained only one exon and the full-length cDNA was 792 bp which encoding 263 amino acids. The ZmEREB211 protein had a molecular weight of 27.9 kD and a theoretical isoelectric point of 6.01. It had a conserved domain unique to the AP2/ERF family. The relative expression level of ZmEREB211 gene was the highest in maize root system and the relative expression level in young roots was higher than mature roots. At the same time, the gene had different degrees of induced expression under dehydration, high salt, drought, and low temperature treatment conditions. On 1/2 MS medium containing different concentrations of NaCl, mannitol, and jasmonic acid (JA), root length of ZmEREB211 transgenic Arabidopsis lines was significantly longer than wild type. Under drought and high salt treatments, transgenic Arabidopsis lines had stronger tolerance than wild type, and the number of green leaves at seedling stage was significantly higher than wild type and the peroxidase (POD) activity and chlorophyll content were significantly higher than wild type. ZmEREB211 may be involved in the regulation of root growth and development in maize, and can play a positive regulatory role in high salt, drought, osmotic stress, and JA hormone treatments. This study provides an important reference for further analysis of the biological function of ZmEREB211 in maize.

Key words: maize, ZmEREB211, transcription factor, heterologous expression, root system, adversity stress

表1

本实验中所用的引物"

引物名称
Primer name		 引物序列
Primer sequence (5'-3')
pZmEREB211-F CTGAAATCACCAGTCGGTACCATGACCAAGAAGCTCATCT (Kpn I)
pZmEREB211-R GCCCTTGCTCACCATGGTACCTCAAAAACTAGACGCGGCG (Kpn I)
pZmEREB211RT-F AGGAGGACGCCATCATCGT
pZmEREB211RT-R GGTACATGTTCCTGTTCCTACG
Actin-F
Actin-R		 TACGAGATGCCTGATGGTCAGGTCA
TGGAGTTGTACGTGGCCTCATGGAC
pGAPDHRT-F
pGAPDHRT-R		 CCCTTCATCACCACGGACTAC
AACCTTCTTGGCACCACCCT

图1

ZmEREB211生物信息学分析 A: 保守结构域分析; B: 跨膜结构预测; C: 蛋白空间结构预测; D: 启动子区顺式作用元件分析。"

图2

ZmEREB211在玉米不同组织中的相对表达量 不同小写字母表示差异显著(P < 0.05)。"

图3

ZmEREB211在不同非生物胁迫下的相对表达量 A~D: ZmEREB211分别在脱水、高盐、渗透、低温处理后的表达量。"

图4

ZmEREB211在玉米不同激素处理中的相对表达量"

图5

T4代拟南芥PCR检测和qPCR检测 A: T4代转基因拟南芥PCR检测; B: T4代转基因拟南芥qPCR检测。M: DL2000 marker; N: 阴性对照; W: 水对照; WT: 野生型拟南芥; L-1~L-6: T4代转基因拟南芥株系; *: P < 0.05; **: P < 0.01。"

图6

不同盐浓度下转基因拟南芥株系根长比较 A~D 分别为0、0.1、0.15和0.18 mol L-1盐处理的生长情况; E: 不同盐浓度下拟南芥株系平均主根长度; WT: 野生型拟南芥; L-3、L-4、L-5: 转ZmEREB211拟南芥株系; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图7

不同甘露醇浓度下转基因拟南芥株系根长比较 A~D 分别为0、0.15、0.2和0.3 mol L-1甘露醇处理的生长情况; E: 不同甘露醇浓度下拟南芥株系平均主根长度。缩写同图6; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图8

不同JA和ABA浓度下转基因拟南芥株系根长比较 A: 0、50、75和100 μmol L-1 JA处理下的生长情况; B: 不同JA浓度下拟南芥株系平均主根长度; C: 0、10、25和50 μmol L-1 ABA处理下的生长情况; D: 不同ABA浓度下拟南芥株系平均主根长度。缩写同图6; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图9

土壤中干旱处理下拟南芥株系表型分析 A: 拟南芥表型; B: 干旱处理后绿叶率; C: 复水处理后绿叶率; D: POD活性的测定; E: 叶绿素含量测定。缩写同图6; *: P < 0.05; **: P < 0.01。"

图10

土壤中高盐处理下拟南芥株系表型分析 A: 拟南芥表型; B: 高盐处理后绿叶率; C: POD活性的测定; D: 叶绿素含量的测定。缩写同图6; *: P < 0.05; **: P < 0.01。"

[1] 仇焕广, 李新海, 余嘉玲. 中国玉米产业: 发展趋势与政策建议. 农业经济问题, 2021, (7): 4-16.
Qiu H G, Li X H, Yu J L. China’s corn industry development trends and policy recommendations. Iss Agric Econ 2021, (7): 4-16. (in Chinese)
[2] Osmond C B, Austin M P, Berry J A, Billings W D, Boyer J, Dacey J W H, Nobel P S, Smith S D, Winner W E. Stress physiology and the distribution of plants: the survival of plants in any ecosystem depends on their physiological reactions to various stresses of the environment. BioScience, 1987, 37: 38-48.
doi: 10.2307/1310176
[3] 张雪莹, 刘欣. 转录因子与叶片发育的研究进展. 植物生理学报, 2022, 58(1): 91-100.
Zhang X Y, Liu X. Research progress of transcription factors and leaf development. Plant Phys J, 2022, 58(1): 91-100. (in Chinese with English abstract)
[4] 葛宝宇, 林轶, 侯和胜. ERF类转录因子的结构与功能. 安徽农学通报, 2007, (20): 32-35.
Ge B Y, Lin Y, Hou H S. Structure and function of ERF transcription factors. AnHui Agric Sci Bull, 2007, (20): 32-35. (in Chinese with English abstract)
[5] 刘强, 张贵友, 陈受宜. 植物转录因子的结构与调控作用. 科学通报, 2000, 45: 1465-1474.
Liu Q, Zhang G Y, Chen S Y. Structure and regulation of plant transcription factors. Chin Sci Bull, 2000, 45: 1465-1474. (in Chinese)
doi: 10.1360/csb2000-45-14-1465
[6] 张艳馥, 沙伟. 转录因子概述. 生物学教学, 2009, 34(10): 7-8.
Zhang Y F, Sha W. Overview of transcription factors. Biol Teach, 2009, 34(10): 7-8. (in Chinese)
[7] 悦曼芳, 张春, 吴忠义. 植物转录因子AP2/ERF家族蛋白结构和功能的研究进展. 生物技术通报, 2022, 38(12): 11-26.
doi: 10.13560/j.cnki.biotech.bull.1985.2022-0432
Yue M F, Zhang C, Wu Z Y. Advances in the structure and function of plant transcription factor AP2/ERF family proteins. Biotechnol Bull, 2022, 38(12): 11-26 (in Chinese with English abstract).
[8] Ng D W K, Abeysinghe J K, Kamali M. Regulating the regulators: the control of transcription factors in plant defense signaling. Int J Mol Sci, 2018, 19: 3737.
doi: 10.3390/ijms19123737
[9] Allen M D, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M. A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J, 1998, 17: 5484-5496.
doi: 10.1093/emboj/17.18.5484 pmid: 9736626
[10] Licausi F, Ohme-Takagi M, Perata P. APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs. New Phytol, 2013, 199: 639-649.
doi: 10.1111/nph.12291 pmid: 24010138
[11] Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration-and cold-inducible gene expression. Biochem Biophys Res Commun, 2002, 290: 998-1009.
doi: 10.1006/bbrc.2001.6299
[12] Ohme-Takagi M, Shinshi H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell, 1995, 7: 173-182.
doi: 10.1105/tpc.7.2.173 pmid: 7756828
[13] Jiang C, Iu B, Singh J. Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus. Plant Mol Biol, 1996, 30: 679-684.
doi: 10.1007/BF00049344 pmid: 8605318
[14] Yamaguchi-Shinozaki K, Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell, 1994, 6: 251-264.
doi: 10.1105/tpc.6.2.251 pmid: 8148648
[15] Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol, 2006, 140: 411-432.
doi: 10.1104/pp.105.073783
[16] Lu L L, Qanmber G, Li J, Pu M L, Chen G Q, Li S D, Liu L, Qin W Q, Ma S Y, Wang Y, Chen Q J, Liu Z. Identification and characterization of the ERF subfamily B3 group revealed GhERF13.12 improves salt tolerance in upland cotton. Front Plant Sci, 2021, 12: 705883.
doi: 10.3389/fpls.2021.705883
[17] Tang M J, Sun J W, Liu Y, Chen F, Shen S H. Isolation and functional characterization of the JcERF gene, a putative AP2/EREBP domain-containing transcription factor, in the woody oil plant Jatropha curcas. Plant Mol Biol, 2007, 63: 419-428.
doi: 10.1007/s11103-006-9098-7
[18] Xie Z L, Nolan T, Jiang H, Tang B Y, Zhang M C, Li Z H, Yin Y H. The AP2/ERF transcription factor TINY modulates brassinosteroid-regulated plant growth and drought responses in Arabidopsis. Plant Cell, 2019, 31: 1788-1806.
doi: 10.1105/tpc.18.00918
[19] Zhang G Y, Chen M, Chen X P, Xu Z S, Li L C, Guo J M, Ma Y Z. Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.). Mol Biol Rep, 2010, 37: 809-818.
doi: 10.1007/s11033-009-9616-1
[20] Zhai Y, Wang Y, Li Y J, Lei T T, Yan F, Su L T, Li X W, Zhao Y, Sun X, Li J W, Wang Q Y. Isolation and molecular characterization of GmERF7, a soybean ethylene-response factor that increases salt stress tolerance in tobacco. Gene, 2013, 513: 174-183.
doi: 10.1016/j.gene.2012.10.018
[21] Tang Y H, Qin S S, Guo Y L, Chen Y B, Wu P Z, Chen Y P, Li M R, Jiang H W, Wu G J. Genome-wide analysis of the AP2/ERF gene family in physic nut and overexpression of the JcERF011 gene in rice increased its sensitivity to salinity stress. PLoS One, 2016, 11: e0150879.
doi: 10.1371/journal.pone.0150879
[22] Jung S E, Bang S W, Kim S H, Seo J S, Yoon H B, Kim Y S, Kim J K. Overexpression of OsERF83, a vascular tissue-specific transcription factor gene, confers drought tolerance in rice. Int J Mol Sci, 2021, 22: 7656.
doi: 10.3390/ijms22147656
[23] Şahin-Çevik M, Moore G A. Two AP2 domain containing genes isolated from the cold-hardy citrus relative Poncirus trifoliata are induced in response to cold. Funct Plant Biol, 2006, 33: 863-875.
doi: 10.1071/FP06005 pmid: 32689297
[24] Zhang J, Liao J Y, Ling Q Q, Xi Y, Qian Y X. Genome-wide identification and expression profiling analysis of maize AP2/ERF superfamily genes reveal essential roles in abiotic stress tolerance. BMC Genomics, 2022, 23: 125.
doi: 10.1186/s12864-022-08345-7 pmid: 35151253
[25] Hao L D, Shi S B, Guo H B, Li M, Hu P, Wei Y D, Feng Y F. Genome-wide identification and expression profiles of ERF subfamily transcription factors in Zea mays. PeerJ, 2020, 8: e9551.
doi: 10.7717/peerj.9551
[26] Wang Z Y, Zhao X, Ren Z Z, Abou-Elwafa SF, Pu X Y, Zhu Y F, Dou D D, Su H H, Cheng H Y, Liu Z X, Chen Y H, Wang E, Shao R X, Ku L X. ZmERF21directly regulates hormone signaling and stress-responsive gene expression to influence drought tolerance in maize seedlings. Plant Cell Environ, 2022, 45: 312-328.
doi: 10.1111/pce.v45.2
[27] Zhang C, Li X L, Wang Z P, Zhang Z B, Wu Z Y. Identifying key regulatory genes of maize root growth and development by RNA sequencing. Genomics, 2020, 112: 5157-5169.
doi: 10.1016/j.ygeno.2020.09.030 pmid: 32961281
[28] 王莉, 王作平, 张中保, 白玲, 吴忠义. 玉米早期籽粒中强表达启动子的筛选. 作物杂志, 2020, (4): 114-120.
Wang L, Wang Z P, Zhang Z B, Bai L, Wu Z Y. Screening of strong expression promoters in early kernels of maize. Crops, 2020, (4): 114-120. (in Chinese with English abstract)
[29] Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735-743.
doi: 10.1046/j.1365-313x.1998.00343.x pmid: 10069079
[30] 杨梦婷, 张春, 王作平, 邹华文, 吴忠义. 玉米ZmbHLH161基因的克隆及功能研究. 作物学报, 2020, 46: 2008-2016.
doi: 10.3724/SP.J.1006.2020.03022
Yang M T, Zhang C, Wang Z P, Zou H W, Wu Z Y. Cloning and functional analysis of ZmbHLH161 gene in maize. Acta Agron Sin, 2020, 46: 2008-2016. (in Chinese with English abstract)
[31] 悦曼芳, 张春, 郑登俞, 邹华文, 吴忠义. 玉米转录因子ZmbHLH91对非生物逆境胁迫的应答. 作物学报, 2022, 48: 3004-3017.
doi: 10.3724/SP.J.1006.2022.13060
Yue M F, Zhang C, Zheng D Y, Zou H W, Wu Z Y. Response of maize transcriptional factor ZmbHLH91 to abiotic stress. Acta Agron Sin, 2022, 48: 3004-3017 (in Chinese with English abstract).
[32] Wang Y Q, Xia D N, Li W Q, Cao X Y, Ma F, Wang Q Q, Zhan X Q, Hu T X. Overexpression of a tomato AP2/ERF transcription factor SlERF.B1 increases sensitivity to salt and drought stresses. Sci Hortic, 2022, 304: 111332.
doi: 10.1016/j.scienta.2022.111332
[33] Peng X J, Ma X Y, Fan W H, Su M, Cheng L Q, Alam I, Lee B H, Qi D M, Shen S H, Liu G S. Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis. Plant Cell Rep, 2011, 30: 1493-1502.
doi: 10.1007/s00299-011-1058-2
[34] Qu Y J, Nong Q D, Jian S G, Lu H F, Zhang M Y, Xia K F. An AP2/ERF gene, HuERF1, from pitaya (Hylocereus undatus) positively regulates salt tolerance. Int J Mol Sci, 2020, 21: 4586.
doi: 10.3390/ijms21134586
[35] Cai X T, Xu P, Zhao P X, Liu R, Yu L H, Xiang C B. Arabidopsis ERF109 mediates cross-talk between jasmonic acid and auxin biosynthesis during lateral root formation. Nat Commun, 2014, 5: 5833.
doi: 10.1038/ncomms6833
[1] 陈国欢, 张锐, 李艳迪, 赵佳琪, 任湧涛, 张天赐, 郭华春, 李俊, 杨芳. 外源硒叶面喷施对浅紫色马铃薯块茎花青素合成的影响[J]. 作物学报, 2026, 52(6): 1876-1890.
[2] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[3] 陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875.
[4] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[5] 王文辕, 燕雪嘉, 刘玉霖, 孙晓彤, 李亚楠, 唐鑫华, 石瑛. 耐弱光马铃薯品种筛选及转录因子编码基因StPIF3的克隆与功能分析[J]. 作物学报, 2026, 52(6): 1631-1645.
[6] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[7] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[8] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[9] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[10] 郭星宇, 胡丹, 林苏期, 王梦凯, 谭文峰, 黄传琴. 生物炭配施化肥提高玉米‖大豆下玉米产量和土壤生态系统多功能性[J]. 作物学报, 2026, 52(5): 1536-1547.
[11] 蔡兆琴, 何观咏, 何文, 阮丽霞, 梁振华, 李永珍, 李恒锐, 陈会鲜. 木薯分枝发育过程的动态转录组分析与关键基因发掘[J]. 作物学报, 2026, 52(5): 1430-1441.
[12] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[13] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[14] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[15] 谷春苗, 王润风, 黄璐, 刘浩, 鲁清, 李海芬, 李少雄, 何双呈, 洪彦彬, 陈小平, 谭斌, 余倩霞. 花生WOX基因家族的全基因组分析及不定芽再生候选基因的鉴定[J]. 作物学报, 2026, 52(5): 1326-1340.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2