Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (06): 1097-1103.doi: 10.3724/SP.J.1006.2008.01097

• RESEARCH NOTES • Previous Articles     Next Articles

Isolation and Sequence Analysis of α-gliadin Genes from Dasypyrum breviaristatum

LI Guang-Rong,REN Zheng-Long*,LIU Cheng,ZHOU Jian-Ping,YANG Zu-Jun*   

  1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China
  • Received:2007-10-01 Revised:1900-01-01 Online:2008-06-12 Published:2008-06-12
  • Contact: YANG Zu-Jun

Abstract: PCR was carried out on the genomic DNA from Dasypyrum breviaristatum using the conserved primers specific forα-gliadin genes. The PCR products were cloned and sequenced. Total 7 sequences were obtained with length of 999 to 1 018 bp containing coding regions from 283 to 290 amino acids and their GenBank accession number are EU186102 to EU186108 respectively. BLAST search showed that these sequences belong to a-gliadin super-family. Searching for 4 different T cell stimulatory epitopes for celiac disease patient, it is found that 5 of 7 sequences contained the Glia-a-2 epitopes at the C terminal region. The phylogenetic trees indicated that all the sequences could not be clustered into the group of a-gliadin genes from the A, B, and D genomes of wheat (Triticum aestivum), but presented in a new group. On the basis of the a-gliadin gene sequences, a pair of AS-PCR primer was designed. PCR results indicated that the AS-PCR primers allow to specifically amplify the D. breviaristatum and wheat-D. breviaristatum partial amphiploid, which thus can be used as a marker to trace the D. breviaristatum derived a-gliadin genes in wheat-Dasypyrum introgression lines.

Key words: Dasypyrum breviaristatum, α-gliadin gene, Gene cloning, Specific PCR

[1] CUI Lian-Hua, ZHAN Wei-Min, YANG Lu-Hao, WANG Shao-Ci, MA Wen-Qi, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping, YANG Qing-Hua. Molecular cloning of two maize (Zea mays) ZmCOP1 genes and their transcription abundances in response to different light treatments [J]. Acta Agronomica Sinica, 2022, 48(6): 1312-1324.
[2] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[3] XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan. Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(1): 108-120.
[4] TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing. Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance [J]. Acta Agronomica Sinica, 2021, 47(4): 672-683.
[5] YUE Jie-Ru, BAI Jian-Fang, ZHANG Feng-Ting, GUO Li-Ping, YUAN Shao-Hua, LI Yan-Mei, ZHANG Sheng-Quan, ZHAO Chang-Ping, ZHANG Li-Ping. Cloning and potential function analysis of ascorbic peroxidase gene of hybrid wheat in seed aging [J]. Acta Agronomica Sinica, 2021, 47(3): 405-415.
[6] YANG Qin-Li, YANG Duo-Feng, DING Lin-Yun, ZHANG Ting, ZHANG Jun, MEI Huan, HUANG Chu-Jun, GAO Yang, YE Li, GAO Meng-Tao, YAN Sun-Yi, ZHANG Tian-Zhen, HU Yan. Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes [J]. Acta Agronomica Sinica, 2021, 47(10): 1854-1862.
[7] HE Xiao, LIU Xing, XIN Zheng-Qi, XIE Hai-Yan, XIN Yu-Feng, WU Neng-Biao. Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate [J]. Acta Agronomica Sinica, 2021, 47(10): 1941-1952.
[8] Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU. Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea [J]. Acta Agronomica Sinica, 2020, 46(12): 1850-1861.
[9] XUE Xiao-Meng,LI Jian-Guo,BAI Dong-Mei,YAN Li-Ying,WAN Li-Yun,KANG Yan-Ping,HUAI Dong-Xin,LEI Yong,LIAO Bo-Shou. Expression profiles of FAD2 genes and their responses to cold stress in peanut [J]. Acta Agronomica Sinica, 2019, 45(10): 1586-1594.
[10] Huan TAN,Yu-Hui LIU,Li-Xia LI,Li WANG,Yuan-Ming LI,Jun-Lian ZHANG. Cloning and Functional Analysis of R2R3 MYB Genes Involved in Anthocyanin Biosynthesis in Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1021-1031.
[11] Jun WANG,Jie-Yu ZHAO,Yang XU,Fang-Jun FAN,Jin-Yan ZHU,Wen-Qi LI,Fang-Quan WANG,Yun-Yan FEI,Wei-Gong ZHONG,Jie YANG. Development and Application of Functional Markers for Rice Blast Resistance Gene Bsr-d1 in Rice [J]. Acta Agronomica Sinica, 2018, 44(11): 1612-1620.
[12] Chen-Yu MA,Wei-Min ZHAN,Wen-Liang LI,Meng-Di ZHANG,Zhang-Ying XI. Cloning and Function Analysis of ZmNAOD Gene in Maize [J]. Acta Agronomica Sinica, 2018, 44(10): 1433-1441.
[13] Chao-Xian LIU, Jiu-Guang WANG, Xiu-Peng MEI, Ting-Ting YU, Guo-Qiang WANG, Lian ZHOU, Yi-Lin CAI. Cloning and Imprinting Characterization Analyses of Paternally Expressed Gene ZmVIL1 in Maize Endosperm [J]. Acta Agronomica Sinica, 2018, 44(03): 376-384.
[14] Yun-Fei LIANG, Lin-Cheng ZHANG, Quan-Ming PU, Zhen-Ze LEI, Song-Mei SHI, Yu-Peng JIANG, Xue-Song REN, Qi-Guo GAO. Cloning of BoLH27 Gene from Cabbage and Phenotype Analysis of Transgenic Cabbage [J]. Acta Agronomica Sinica, 2018, 44(03): 397-404.
[15] LUO Yun, MA Xuan, GU Jun-Chen, YAN Hai-Fang*. Cloning, Location and Expression of BrSIZ1 in Brassica rapa L. subsp.rapifera ‘Tsuda’ [J]. Acta Agron Sin, 2018, 44(01): 75-81.
Full text



No Suggested Reading articles found!