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Acta Agron Sin ›› 2018, Vol. 44 ›› Issue (01): 75-81.doi: 10.3724/SP.J.1006.2018.000075

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning, Location and Expression of BrSIZ1 in Brassica rapa L. subsp.rapifera ‘Tsuda’

LUO Yun, MA Xuan, GU Jun-Chen,YAN Hai-Fang*   

  1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China
  • Received:2017-03-07 Revised:2017-07-23 Online:2018-01-12 Published:2017-08-02
  • Contact: 闫海芳, E-mail: yanhaifang224@126.com
  • Supported by:

    This study was supported by the Fundamental Research Funds for the Central Universities and the Research Training Program of Northeast Forestry University.

Abstract:

SIZ1, a SUMO E3 ligase involved in post-translation of proteins in plant cells, plays a role in protein interaction、location and response to environmental stresses. In order to elucidate the expression profile of SIZ1 in Tsuda, cDNA of SIZ1 gene was isolated from Tsuda. This gene was named BrSIZ1 (GenBank accession number KY441465). BrSIZ1 was 2754 bp in full length cDNA and 2571 bp in full length open reading frame (ORF), encoding a peptide with 856 amino acids. A BrSIZ1-GFP expression vector was constructed to analysis the subcellular localization. BrSIZ1-GFP was localized to nucleus, indicating that BrSIZ1 may play an important role in the nucleus. Quantitative-PCR analysis showed that the BrSIZ1 was expressed meetly in leaf and secondly in young seedling and red root epidermis, showing tissue specificity. The expression of the BrSIZ1 was induced by UV-A light in the root epidermis. The transcript level of BrSIZ1 was up-regulated when treated with temperature of 4°C or 37°C in young seedling.

Key words: Brassica rapa, SIZ1, gene cloning, location, expressing analysis

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