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Acta Agron Sin ›› 2018, Vol. 44 ›› Issue (01): 82-94.doi: 10.3724/SP.J.1006.2018.00082

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Mechanism on Drought Tolerance Enhanced by Exogenous Glucose in C4-pepc Rice

ZHANG Jin-Fei1, 2, LI Xia1,*, HE Ya-Fei1, 2,XIE Yin-Feng2   

  1. 1 Institute of Food Crops, Jiangsu Rice Engineering Research Center, National Center for Rice Improvement (Nanjing), Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China; 2 College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China
  • Received:2017-03-28 Revised:2017-09-10 Online:2018-01-12 Published:2017-09-28
  • Contact: 李霞, E-mail: jspplx@jaas.ac.cn E-mail:820788317@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31571585), the Jiangsu Provincial Academy of Agricultural Sciences Basic Research Business Special Project (ZX [16] 2002), and the Grant from the Institute of Food Crops of Jiangsu Academy of Agricultural Sciences (LZS17-9).

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Abstract:

In order to investigate the intrinsic mechanism of glucose participated in drought tolerance in plants, the effects of glucose were studied using the phosphoenolpyruvate carboxylase (C4-pepc) rice (PC) and “Kitaake” (WT) rice lines in pot experiments and hydroponics experiments respectively. The changes of photosynthetic parameters, total soluble sugar and sugar components contents, Ca2+ and NO contents, hexokinase activity, transcript levels of sucrose nonfermenting-1(SNF1)-related protein kinases 3 (SnRK3s) and calcitonin B-like (CBL) of the functional leaves in rice lines were measured. Agronomic traits of the wild type (WT) and PC were recorded in the mature period. In pot experiment, the treatment of 3% glucose with drought during tillering stage had no significant effect on agronomic traits of the tested rice. During the booting stage, the plant height, panicle number per plant, filled grain number per panicle and grain yield per plant in PC were significantly higher than in WT (P < 0.05). In the hydroponics experiment with 1% glucose combined with 12% (m/v) polyethylene glycol 6000 (PEG-6000) to simulate drought stress, the photosynthetic parameters such as net photosynthetic rate (Pn), stomatal conductance (Gs) and carboxylation efficiency (Ce) significantly increased in PC than in WT. Similarly, the contents of sucrose and fructose of leaves in PC lines were significantly higher than those in WT. It was noteworthy that hexokinase (HXK) activity and the relative gene expression of CBL and SnRK3.1/SnRK3.4/SnRK3.21 in PC lines under the treatment with 1% glucose and 12% PEG were significantly lower than those under 12% PEG treatment alone. Intriguingly, the NO contents of PC under the corresponding treatments were significantly increased (P < 0.05). In addition, the photosynthetic parameters were significantly correlated with the glucose content, HXK activity and SnRK3.16 transcript level respectively in PC lines. It is suggested that PC can decrease the expression of CBL and SnRK3s gene by increasing glucose, participate the stomatal regulation via NO, maintain relative water content, keep stable photosynthetic capacity, and therefore confer drought tolerance.

Key words: rice, glucose, phosphate phosphoenolpyruvate carboxylase, stomatal conductance, drought

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