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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 255-261.doi: 10.3724/SP.J.1006.2009.00255

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Mapping and Interaction Analysis for 1000-Grain Weight and Percentage of Grains with Chalkiness in Rice

ZHOU Li-Jun1,JIANG Ling1,LIU Xi1,CHEN Hong1,CHEN Liang-Ming1,LIU Shi-Jia1,WAN Jian-Min1,2,*   

  1. 1State Key Laboratory of Crop Genetics and Germplasm enhancement/Jiangsu Plant Gene  Engineering Research Center, Nanjing Agricultural University, Nanjing 210095,China;2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081,China
  • Received:2008-07-31 Revised:2008-10-08 Online:2009-02-12 Published:2008-12-10
  • Contact: WAN Jian-Min

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Abstract:

There is a close correlation between 1000-grian weight (TGW, an important yield factor) and percentage of grains with chalkiness (PGWC, an important rice quality index). In this study, a backcross inbred lines (BIL) population derived from a cross between Koshihikari (japonica) and Kasalath (indica) was used to detect correlations and among interactions QTL, epistatic and environment on TGW and PGWC. Correlation analysis showed that there was a significantly positive correlation between TGW and PGWC in the BIL population and the correlation coefficients were 0.42 and 0.35 (P<0.001) in 2005 and 2006, respectively. A total of eleven QTLs and eight epistatic interactions for TGW were detected in 2005 and 2006; of them, five QTLs were repeatedly detected in the two years, and five QTLs and seven epistatic interactions had significantly QE interaction. A total of six QTLs and nine epistatic interactions for PGWC were detected in 2005 and 2006; of them, three QTLs and four epistatic interactions had markedly QE interaction. Three main-effect QTLs simultaneously controlling TGW and PGWC were detected, and their alleles increasing TGW and PGWC were from the same parent; one epistatic interaction had similar effects on TGW and PGWC. Some main-effect QTLs, controlling TGW but not PGWC, such as qTGW-3c, qTGW-4a,and qTGW-6b, could be used for breeding. The strategy was discussed in using QTL mapping results for the marker-assisted selection breeding of TGW and PGWC.

Key words: Rice, 1000-grain weight (TGW), Percentage of grains with chalkiness(PGWC), QTL, epistatic interaction, QTLXenvironment interaction(QE)

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